Compound pesticide containing cyprodinil and ningnanmycin
A kind of technology of ningnanmycin and cyprodinil, applied in the field of pesticides
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Embodiment 1
[0045] Embodiment 1 The preparation of the composition containing Ningnanmycin
[0046] Inoculate the frozen Streptomyces noursii var. Xichang (purchased from Heilongjiang Biopesticide Engineering Technology Research Center) on a freshly prepared solid medium, at 28 ° C, 80% relative humidity, and under the condition of sterile air circulation Cultured for 16 days for rejuvenation, and the resulting strain spores were picked. Then produce mycelium, that is, to inoculate the spores of the strain on the freshly prepared solid medium, and culture it aerobically at 28°C, 80% relative humidity, and under the condition of sterile air circulation for 14 days, and the mycelium can be clearly seen produce. Carry out proliferation culture then, promptly use the first freshly prepared liquid medium to wash mycelia from the surface of the solid medium and enter a 1L Erlenmeyer flask, add the first freshly prepared liquid medium to supplement to a medium volume of 250ml, Incubate for 14 ...
Embodiment 2
[0050] The formula of embodiment 2 compound pesticides
[0051]1,50% cyprodinil+5% Ningnanmycin wettable powder (WP): get cyprodinil 50g; Ningnanmycin 5g prepared in Example 1; sodium lauryl sulfate (wetting agent ) 2g; sodium lignosulfonate (dispersant) 4g; sodium methylene dinaphthalene sulfonate (NNO, dispersant) 5g; kaolin (filler) to make up to 100g, crushed into wettable powder.
[0052] 2. 38% cyprodinil + 2% ningnanmycin suspension concentrate (SC): take cyprodinil 38g; ningnanmycin 2g prepared in Example 1; sodium lignosulfonate (dispersant) 3g; Sodium methylene dinaphthalene sulfonate (NNO, dispersant) 2g; Ethylene glycol (antifreeze) 4g; Xanthan gum (thickener) 0.2g; Silicone oil (defoamer) 0.3g; 100g.
[0053] 3, 20% cyprodinil+5% ningnanmycin microemulsion (ME): get cyprodinil 20g; Ningnanmycin 5g prepared in Example 1; acetone (solvent) 30g; dodecyl Calcium benzenesulfonate (emulsifier) 4g; Styrylphenol polyoxyethylene ether (emulsifier) 6.5g; Tristyrylphe...
Embodiment 3
[0056] The control effect of embodiment 3 compound pesticides
[0057] We used 38% cyprodinil+2% Ningnanmycin suspension (SC1) prepared according to Example 2 and an equivalent amount of commercially available 50% cyprodinil suspension (purchased from Yantai Keda Chemical Co., Ltd.) (SC2) and 38% cyprodinil+2% Ningnanmycin suspension concentrate (SC3) prepared with the original drug; it refers to the corresponding formula of Example 2, but with the original drug of Ningnanmycin (purchased from Heilongjiang Qianger Biochemical Technology Development Co., Ltd.) instead) to do field experiments to show its control effect on various crops.
[0058] (1) Field efficacy test of rice blast
[0059] In the high-incidence area of Jiangsu Province, 2 acres of rice test field were divided into equal parts, and each area was tested with SC1, SC2, SC3 and blank control (CP). After 7 days, the same amount of pesticide was applied again, and the effect of controlling rice blast was observ...
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