Compound pesticide containing cyprodinil and ningnanmycin
A kind of technology of ningnanmycin and cyprodinil, applied in the field of pesticides
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[0045] Example 1 Preparation of Ningnanmycin-containing composition
[0046] The cryopreserved Streptomyces knowles Xichang variety (purchased from Heilongjiang Biopesticide Engineering Technology Research Center) was inoculated on a freshly prepared solid medium at 28°C, 80% relative humidity, and sterile air circulation. Cultivate for 16 days for rejuvenation, and pick out the produced spores. Then the mycelium is produced, that is, the spores of the strain are inoculated on a freshly prepared solid medium, and aerobic culture is carried out at 28°C, 80% relative humidity, and sterile air circulation for 14 days. The mycelium is clearly visible produce. Then carry out multiplication culture, that is, wash the mycelium from the surface of the solid medium with the freshly prepared first liquid medium into a 1L Erlenmeyer flask, add the freshly prepared first liquid medium to supplement the medium volume to 250ml, Incubate at 28°C and shaker at 250 rpm for 14 days. After sampl...
Example Embodiment
[0050] Example 2 Formula of compound pesticide
[0051] 1. 50% Cyprodinil + 5% Ningnanmycin Wettable Powder (WP): Take 50g Cyprodinil; 5g Ningnanmycin prepared in Example 1; Sodium lauryl sulfate (wetting agent) ) 2g; sodium lignosulfonate (dispersant) 4g; sodium methylene dinaphthalene sulfonate (NNO, dispersant) 5g; kaolin (filler) is made up to 100g and crushed into a wettable powder.
[0052] 2. 38% cyprodinil + 2% ningnanmycin suspension (SC): take 38g cyprodinil; 2g ningnanmycin prepared in Example 1; sodium lignosulfonate (dispersant) 3g; Sodium methylene dinaphthalene sulfonate (NNO, dispersant) 2g; ethylene glycol (antifreeze) 4g; xanthan gum (thickener) 0.2g; silicone oil (defoamer) 0.3g; deionized water to make up to 100g.
[0053] 3. 20% cyprodinil + 5% ningnanmycin microemulsion (ME): take 20g cyprodinil; 5g ningnanmycin prepared in Example 1; acetone (cosolvent) 30g; dodecyl Calcium benzenesulfonate (emulsifier) 4g; phenethylphenol polyoxyethylene ether (emulsifier...
Example Embodiment
[0056] Example 3 Control effect of compound pesticide
[0057] We use 38% cyprodinil + 2% cyprodinil suspension (SC1) prepared according to Example 2 and the same amount of 50% cyprodinil suspension (purchased from Yantai Keda Chemical Co., Ltd.) (SC2) and 38% cyprodinil+2% Ningnanmycin suspension prepared with the original drug (SC3; refer to the corresponding formulation in Example 2, but use the original drug of Ningnanmycin (purchased from Heilongjiang Qiang'er Biochemical Technology Development Co., Ltd.) instead of) do field test comparisons to show its control effects on various crops.
[0058] (1) Field efficacy test of rice blast
[0059] In the high-incidence area of Jiangsu Province, 2 mu of rice test fields were equally divided, and SC1, SC2, SC3 and a blank control (CP) were used to test one area each. The rice planted in it was sprayed in the same amount in the first ten days of August at intervals. After 7 days, the same amount of pesticide was applied again, and t...
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