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Method for preparing fissistigma total flavonoids and fissistigma total alkali

A technology of Fructus Fructus and total flavonoids is applied in the field of preparing total flavonoids and total alkaloids of Fructus Fructus, can solve the problems of large amount of organic solvent, low utilization rate of raw materials, low purity of Fructus Fructus total alkaloids, etc., and achieves low solvent toxicity. , High utilization rate of raw materials, low environmental pollution

Inactive Publication Date: 2011-09-14
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method uses a large amount of organic solvents, and the purity of the total alkaloids of guafora sinensis is not high
For example, the master's thesis "Studies on the Chemical Components and Preliminary Study on the Antibacterial Activity of Melaleuca sinensis", the method disclosed in this paper is to use 95% ethanol to reflux extraction, concentrate the extract, dissolve it with 1% hydrochloric acid, and then add ammonia water to the solution to adjust the pH to 8-9. Add chloroform for extraction, and finally recycle chloroform to obtain total alkaloids. This method consumes a lot of organic solvents and the utilization rate of raw materials is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1kg of the medicinal material of Fructus chinensis, crush it, add 4L of 70% ethanol to reflux for 2 times, each time for 2h, combine the extracts, concentrate until there is no alcohol smell, take 350g of AB-8 macroporous resin and pack it into a column. Resin column adsorption, wash with a small amount of water earlier, then use 3BV 80% ethanol elution, collect lower column liquid, water washing liquid and alcohol washing liquid (1) respectively; HD-8 type cation resin column chromatography, eluted with an ammonia solution of pH 9, collected the alkaline washing solution (II), removed the solvent from the alcohol washing solution (I) and the alkaline washing solution (II), respectively, and vacuum-dried to obtain Fructus Fructus The total flavonoids are 5.4g and the total alkaloids of cucurbita 4.3g, and the content is not less than 60%.

Embodiment 2

[0021] Take 2kg of the medicinal materials of Guafu wood, pulverize them, add 20L 50% ethanol for reflux extraction for 4 times, 0.5h each time, combine the extracts, concentrate until there is no alcohol smell, take 1.5kg of HPD-450 type daza resin and pack it into a column, add the concentrated solution to the Macroporous resin column adsorption, first wash with a small amount of water, then use 6BV 60% ethanol elution, collect lower column liquid, water washing liquid and alcohol washing liquid (I) respectively; Lower column liquid and water washing liquid are combined, and adjusted to pH4 with dilute hydrochloric acid , go up 732 type cation resin column chromatography, the ammonia solution of pH11 is eluted, collect the alkaline washing solution (II), the alcohol washing solution (I) and the alkaline washing solution (II) are removed the solvent respectively, and spray-drying promptly obtains Fructus Fructus 12.3 g of total flavonoids and 7.9 g of total alkaloids of Cucurb...

Embodiment 3

[0023] Take 5kg of Fructus melon, crushed, add 30L of 60% ethanol to reflux for 3 times, 1 hour each time, combine the extracts, concentrate until there is no alcohol smell, take 2.5kg LSA-20 macroporous resin and pack it into a column, and the concentrated solution Pore ​​resin column adsorption, first wash with a small amount of water, then use 5BV 65% ethanol elution, collect lower column liquid, water washing liquid and alcohol washing liquid (I) respectively; Lower column liquid and water washing liquid are combined, and adjusted to pH3 with dilute hydrochloric acid, On HD-8 type cation resin column chromatography, the ammonia solution of pH10 is eluted, the alkaline washing solution (II) is collected, the alcohol washing solution (I) and the alkaline washing solution (II) are respectively removed from the solvent, and freeze-drying is to obtain the guafu 20.3 g of total flavonoids of wood and 19.8 g of total alkaloids of Citrus chinensis, the contents of which are not les...

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Abstract

The invention provides a method for preparing fissistigma total flavonoids and fissistigma total alkali. The method is characterized by comprising the following steps: crushing a fissistigma medicinal material; refluxing and extracting for 2 to 4 times by adopting 50-70 percent ethanol; combining extracts, concentrating until the mixture does not have alcohol smell; adsorbing by a macroporous resin column; collecting residual solution and alcohol lotion (I) respectively; adjusting the residual solution to be acidic with diluted hydrochloric acid, and chromatographing on a cationic resin column; and collecting alkaline lotion (II), removing solvent of the alcohol lotion (I) and the alkaline lotion (II) respectively to obtain the fissistigma total flavonoids and the fissistigma total alkali. In the extracts of the fissistigma total flavonoids and the fissistigma total alkali simultaneously prepared by adopting the method, the raw material utilization is high, the preparation process is safe, the cost is low, the period is short, and the contents of the prepared fissistigma total flavonoids and fissistigma total alkali are both over 60 percent. Therefore, the method has industrial significance.

Description

Technical field: [0001] The invention relates to the field of separation of natural products, in particular to a method for preparing total flavonoids and total alkaloids of Cucurbita chinensis from Cucurbita sinensis. Background technique: [0002] Fissistigma oldhamii (Hemsl.) Merr., also known as iron bull diamond, diamond three winds, fragrant vine, is a plant of the genus Fissistigma Griff. . Mainly produced in Zhejiang, Fujian, Taiwan, Jiangxi, Hunan, Guangdong, Hainan, Guangxi and Yunnan provinces and cities. Its roots, stems, skins, and flowers are all medicinal, warm in nature and pungent in taste. According to reports in the literature, guava wood contains alkaloids, flavonoids, organic acids, anthraquinones and other chemical components. [0003] The physiological activities of Cucurbita are mainly reflected in anti-tumor, cardiovascular activity, anti-inflammatory, analgesic and smooth muscle relaxation. Pharmacological experiments have shown that the total a...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P29/00A61P7/02A61P19/02A61P25/02A61K125/00A61K129/00A61K133/00A61K135/00
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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