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Blueberry tissue culture method and special culture medium thereof

A medium and a subculture medium technology, applied in the field of plant tissue culture, can solve problems such as unseen large-scale industrial production, and achieve the effects of shortening the culture period and low cost

Inactive Publication Date: 2012-11-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic blueberry seedling breeding is still in its infancy, and there are many problems, and there are no large-scale factory production reports

Method used

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  • Blueberry tissue culture method and special culture medium thereof
  • Blueberry tissue culture method and special culture medium thereof
  • Blueberry tissue culture method and special culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Preparation and statistical observation of regenerated plants of "Heyun 1"

[0050] 1. Preparation and statistical observation of the first-generation tissue culture seedlings of "Heyun No. 1"

[0051] 1. The acquisition and pretreatment of explants

[0052] 1) Selection of explants

[0053] In this embodiment, blueberry tissue culture adopts axillary bud proliferation. From June to September each year, in the "Heyun No. 1" blueberry seed resource nursery (purchased from Jikang Company of Jilin), select explants from blueberry seedlings that are free from pests and diseases, have good tree shapes, good fruit characteristics, and high yield. The explants are selected from the new shoots that year, with 6-10 buds on one branch.

[0054] 2) Pretreatment of explants

[0055] Put the explants cut off in the above step 1 into a beaker filled with water, drip 4-6 drops of detergent, gently scrub the axillary buds and stems of the branches with a soft brush, and then remove t...

Embodiment 2

[0097] Example 2. Preparation and statistical observation of the first generation tissue culture seedlings of "Heyun 1"

[0098] The difference between this example and Example 1 is that the final concentration of zeatin in the primary culture medium is adjusted to 4 mg / L, and the rest remains unchanged.

[0099] The statistical observation results are as follows:

[0100] 1) Primary training

[0101] In the first-generation medium of Example 2 above, the blueberry shoots with axillary buds can be observed when cultured for 55-65 days. The experiment was repeated 3 times. After 60 days of culture, the induction of axillary buds was statistically observed. The results are shown in Table 4 below, and the bud induction rate can reach more than 93%.

[0102] Table 4. Induction rate of axillary buds

[0103] Repeat

[0104] 2) Subculture

[0105] The primary tissue culture seedlings or secondary seedlings inoculated into the secondary culture medium in the above example 2 were cultured for...

Embodiment 3

[0109] Example 3. Preparation and statistical observation of the first generation tissue culture seedlings of "Heyun 2"

[0110] The difference between this example and Example 1 is that the stem segment with axillary buds of blueberry "Heyun No. 2" is used as explants for culture, and the NAA of the primary medium and the ZT, NAA, and IBA of the secondary medium are different , The rest of the steps are the same as in Example 1.

[0111] In this embodiment:

[0112] (1) "Heyun No. 2" blueberries were purchased from Jilin Jikang Company.

[0113] (2) The final concentration of NAA in the primary culture medium is 0.1 mg / L.

[0114] (3) The final concentration of zeatin (ZT) in the subculture medium is 3 mg / L, the final concentration of NAA is 0.06 mg / L, and the final concentration of IBA is 0.3 mg / L.

[0115] The statistical observation results are as follows:

[0116] 1) Primary training

[0117] In the first-generation medium of Example 3, the stem segments of blueberry with axillary bu...

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Abstract

The invention discloses a blueberry tissue culture method and a special culture medium thereof. The special culture medium is a primary culture medium for blueberry tissue culture and is a solid culture medium which is obtained by adding zeatin, naphthyl acetic acid (NAA), a carbon source and gels into basic culture solution; and in the primary culture medium, the zeatin has the final concentration of 2-5mg / L, the NAA has the final concentration of 0.01-0.12mg / L, and the solvent of the basic culture solution is water and solute of the basic culture solution is shown as Table 1. Experiments prove that: the primary culture medium can greatly shorten the culture period of the tissue culture from three months to two months, the inductivity of sprouts is over 90 percent, and the production capacity of tissue culture each year is over 3 million seedlings. The method has the characteristics of high efficiency and time conservation, the produced tissue culture seedlings are low in cost, the average cost of each seedling is 1.5 yuan, the obtained blueberry tissue culture seedlings have consistent inherited characteristics, and the defect that the conventional reproduction coefficient is low is overcome.

Description

Technical field [0001] The invention relates to the field of plant tissue culture, in particular to a blueberry tissue culture method and a special primary culture medium. Background technique [0002] Blueberry (blueberry) is a perennial shrub belonging to the genus Vaccinium (Vaccinium SPP.) of the azalea family (Ericaceae). The fruit is a small blue-purple berry with a layer of white fruit powder. It is sweet and sour and has a unique flavor. It can be eaten fresh or deep-processed. It is rich in A, Vpp, Vp, and VB. 1 , VB 2 , VB 5 , D, E, K and other vitamins, organic germanium, organic selenium and other trace elements required by the human body, as well as arbutin, anthocyanin, SOD, ellagic acid, and flavonoids and other antioxidant components, high Fiber, low-calorie, its nutritional value is much higher than that of ordinary fruits, especially the anthocyanin content of blue berries ranks first among various fruits and vegetables. Medical research has proved that anthocy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 孟凡乔吴文良李磊刘凝殷秀岩郭岩彬杨景娜
Owner CHINA AGRI UNIV
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