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Square wave volt-ampere method for detecting vitamin content in blood samples

A square wave voltammetry and vitamin technology, which is applied in the field of square wave voltammetry to detect the vitamin content of blood samples, can solve the problems of complicated detection methods, long detection time, high price, etc., and achieves short test time, high accuracy, Easy-to-use effects

Active Publication Date: 2013-07-03
天津市兰标电子科技发展有限公司
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  • Application Information

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Problems solved by technology

Due to high-performance liquid chromatography, fluorescence analyzer and other instruments are expensive, and require specialized technical personnel to operate, the detection method is complicated, and the detection time is long, so it is not easy to popularize

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  • Square wave volt-ampere method for detecting vitamin content in blood samples

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Embodiment 1

[0028] Square wave voltammetry for detecting vitamin content in blood samples: Take a certain amount of sample to be tested and mix it with a certain amount of self-made vitamin sample treatment solution, and use square wave voltammetry to oxidize the vitamins in the sample to be tested on the self-made sensor probe (reduction) reaction, generating a current signal. By comparing the current signal values ​​of vitamin standard samples with different concentrations, a standard curve is developed, and the vitamin content in the sample to be tested is obtained according to the current signal value generated by the sample to be tested.

[0029] The specific implementation process of detecting vitamin B9 content in blood samples:

[0030] 1. Preparation of vitamin B9 sample treatment solution: liquid vitamin B9 sample treatment solution is composed of tartrate, acetate buffer solution, surfactant, ionic strength regulator, strong acid and dimethylglyoxime ethanol solution; tartrate...

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Abstract

The invention, which relates to a square wave volt-ampere method for detecting the vitamin content in blood samples, belongs to the technical field of vitamin analysis and detection. The square wave volt-ampere method for detecting the vitamin content in blood samples is characterized by mixing blood samples to be measured with a vitamin sample treatment fluid; detecting current signals generatedwhen the sample is subjected to an oxidation reduction reaction on the sensor probe of a vitamin detector by using the square wave volt-ampere method; making a standard curve by comparing current signal values of standard samples with different vitamin concentrations; and acquiring the vitamin content in the blood samples to be measured according to current signal values generated by the samples to be measured. The method provided by the invention has high sensitivity and good accuracy, is easy and fast to operate, can be widely applied, is appropriate to be used in medical health departments, and can be adopted to analyze and detect vitamin A, B1, B2, B6, B9, B12, C, D, E, K1 and K3 in blood samples and rapidly detect the vitamin content in blood samples.

Description

technical field [0001] The invention belongs to the technical field of vitamin analysis and detection, in particular to a square wave voltammetry method for detecting vitamin content in blood samples. Background technique [0002] Vitamins are a class of trace organic substances that the body must ingest from food in order to maintain normal physiological functions, and play an important role in regulating substance metabolism and maintaining physiological functions. Long-term lack or excessive intake of certain vitamins can lead to corresponding diseases. For example, night blindness, dry eyes, and dry skin can occur due to vitamin A deficiency; rickets can occur due to vitamin D deficiency; pernicious anemia can occur due to vitamin B12 deficiency; excessive intake of vitamin E can damage blood coagulation and increase the possibility of bleeding; intake of more than 1000 mg of vitamin C can affect the detoxification function of the kidneys. [0003] At present, there ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48
Inventor 范清杰
Owner 天津市兰标电子科技发展有限公司