Microbial organic manure and preparation method thereof
A technology of microbial organic fertilizer and microbial bacterial fertilizer, applied in the direction of organic fertilizer, biological organic part treatment, etc., can solve the problems of ecological balance destruction, reduction of chemical fertilizer utilization rate, nitrogen fertilizer waste, etc., to stimulate plant growth, increase supply capacity, enhance The effect of disease resistance
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Embodiment 1
[0029] 1) Strain culture: No. 1 bacterial liquid yeast and Bacillus subtilis culture liquid are Saccharomyces cerevisiae ( saccharomyces cerevisiae ), Bacillus subtilis ( Bacillus subtilis ), mixed and inoculated in the culture medium at a volume ratio of 1:1, and anaerobically cultured at 30°C for 48 hours. 4 5.0‰, the balance is 1000ml distilled water, pH 6.5-7.0;
[0030] Actinomycetes culture solution of No. 2 bacteria is actinomycetes huminosa ( Actinomyces humiferus ) aerobically cultivated in the culture medium at 25-40℃ for 60 hours, wherein the culture medium is, by weight, 60‰ flour, and the balance is 1000ml distilled water pH 7.0;
[0031] No. 3 bacterial liquid nitrogen-fixing bacteria culture medium is brown nitrogen-fixing bacteria ( Azotobacter vinelandii ) in culture medium 25-40 ℃ aerobic culture for 48h, wherein the culture medium is, by weight, KH 2 PO 4 0.2‰, MgSO 4 ·7H 2 O 0.2‰, NaCl 0.2‰, CaCO 3 5.0‰, C 6 h 14 o 6 10.0‰, CaSO 4 2H 2 O...
Embodiment 2
[0038] 1) Strain culture: strain culture: No. 1 bacterial liquid yeast and Bacillus subtilis culture liquid are Saccharomyces cerevisiae ( saccharomyces cerevisiae ), Bacillus subtilis ( Bacillus subtilis ), mixed inoculated in the culture medium at 1:1, anaerobically cultured at 30°C for 48h, and the culture medium was, by weight, corn starch 20.0‰, yeast extract 15.0‰, soybean meal 20.0‰, MgSO 4 5.0‰, the balance is 1000ml distilled water, pH 6.5-7.0;
[0039] Actinomycetes culture solution of No. 2 bacteria is actinomycetes huminosa ( Actinomyces humiferus ) aerobically cultivated in the culture medium at 25-40℃ for 60 hours, wherein the culture medium is, by weight, 60‰ flour, and the balance is 1000ml distilled water pH 7.0;
[0040] No. 3 bacterial liquid nitrogen-fixing bacteria culture medium is brown nitrogen-fixing bacteria ( Azotobacter vinelandii ) in culture medium 25-40 ℃ aerobic culture for 48h, wherein the culture medium is, by weight, KH 2 PO 4 0.2‰,...
Embodiment 3
[0046] Taking greenhouse tomato as the test material, two treatment groups were designed, the control group using common compost and the test group using microbial organic fertilizer. Both fertilizers were used as base fertilizer, and the usage rate was 3000kg / mu. Each experimental treatment group set 4 repetitions, each repetition 100m 2 , routine management. Observe the influence of microbial organic fertilizer on tomato yield, see Table 1 for details.
[0047] Table 1 Effect of application of microbial organic fertilizer on tomato yield
[0048] deal with
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