Duck egg yolk antibody for preventing and treating virus hepatitis of duckling, and preparation method thereof
A technology of viral hepatitis and yolk antibody, which is applied in the field of preparing duck yolk antibody for preventing and treating duckling viral hepatitis, and the field of duck yolk antibody for preventing and treating duckling viral hepatitis. , The genetic background of heterogeneous ducks is not clear, the level of yolk antibody decreases, etc., to achieve the effect of good performance and control effect, low market price, and high level of yolk antibody
Active Publication Date: 2014-02-19
SHUNHUA DUCK INDAL DEVMET LINWU COUNTY HUNAN
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Problems solved by technology
The yolk antibody currently used clinically is extracted from the eggs of general high-free laying ducks by commercial production units. The following risks exist when ducklings in different places use this yolk antibody, and it is not safe to use: genetic background of heterogeneous duck populations Unclear, maybe carrying bad genes or potential vertical transmission of pathogens, such as avian leukemia, etc.; the level of antibodies in high-free laying ducks is unknown, maybe there are incompatible strains
In addition, if the laying duck lays eggs for a long time, the level of yolk antibody will decrease, and the performance and effect will be reduced
Moreover, ordinary laying ducks lay large eggs and have a high market price, but when used to prepare yolk antibodies, the titer is low and the economic value is not high
Method used
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[0022] To prepare the duck yolk antibody for preventing and treating duckling viral hepatitis of the present invention, the preparatory work has strict requirements:
[0023] 1. Preparation of normal saline: pure water containing 0.85% NaCl, autoclaved at 1 kg / ㎝2 pressure for 20 minutes or boiled for 20 minutes to sterilize, let cool for later use, add gentamicin to make the final concentration of gentamicin after dilution to 100 IU / mL, add formaldehyde to make the final concentration 0.08%.
[0024] 2. Prepare the finished egg yolk antibody bottle: after cleaning, wrap the mouth of the bottle with kraft paper, dry heat disinfection at 150°C for 1 hour, and set aside, or use a disposable plastic bottle.
[0025] 3. Prepare rubber stoppers, 50-mesh filter membranes, gauze, etc., and dry them after autoclaving at 15 pounds for 20 minutes.
[0026] 4. Other utensils should be boiled or steam sterilized for 20 minutes before production.
[0027] 5. The sterile room for productio...
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The invention discloses a duck egg yolk antibody for preventing and treating virus hepatitis of ducklings, and a preparation method thereof. The duck egg yolk antibody is extracted from the primiparous eggs of a high-immunity homebred breed duck starting laying for one month. The preparation method comprises the following steps of: (a) highly immunizing the breed duck before laying; (b) collecting high-immunity primiparous breed duck eggs; (c) cleaning and sterilizing; (d) separating egg yolks; (e) diluting; (f) stirring and filtering; and (g) packaging and preserving. After the egg yolks are continuously diluted for 2 times by using normal saline, the DHV (Duck Hepatitis Virus) precipitating antibody is detected by a conventional method, the antibody titer is more than 1:64, and the HI titer of H5 is more than 1:512. 0.5mL of the duck egg yolk antibody is subcutaneously injected to a 3-day-old duckling for preventing virus hepatitis; and 1mL of egg yolk antibody to which antibiotics are added is subcutaneously injected to a duck for treating virus hepatitis, and the egg yolk antibody is re-injected to a seriously diseased duck every other day.
Description
technical field [0001] The invention relates to a yolk antibody, in particular to a duck yolk antibody for preventing and treating duckling viral hepatitis; and also relates to a method for preparing the duck yolk antibody for preventing and treating duckling viral hepatitis. Background technique [0002] Duck viral hepatitis is the most common viral disease in ducklings, and high immunity to yolk antibodies is the most reliable way to treat duckling viral hepatitis. The yolk antibody currently used clinically is extracted from the eggs of general high-free laying ducks by commercial production units. The following risks exist when ducklings in different places use this yolk antibody, and it is not safe to use: genetic background of heterogeneous duck populations Unclear, it may carry bad genes or potential vertically transmitted pathogens, such as avian leukaemia, etc. The antibody level of high-free laying ducks is unknown, and there may be strains that are not compatible....
Claims
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IPC IPC(8): C07K16/10C07K16/02
Inventor 古飞霞骆小龙刘炎圭邝文涛
Owner SHUNHUA DUCK INDAL DEVMET LINWU COUNTY HUNAN