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A porcine hepatitis E virus antigenic epitope and its application

A technology for swine hepatitis E and virus antigen, which is applied in the directions of viral peptides, antiviral agents, and medical preparations containing active ingredients, etc., can solve the problems of not being able to specifically reflect the epidemic pattern of swine hepatitis E, and the specificity is not strong.

Inactive Publication Date: 2011-12-14
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although porcine hepatitis E virus and human hepatitis E virus have a high sequence homology, there are still some differences between them. Using human hepatitis E virus epitopes to detect porcine hepatitis E virus leads to low detection specificity. Can not specifically reflect the prevalence of swine hepatitis E

Method used

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  • A porcine hepatitis E virus antigenic epitope and its application
  • A porcine hepatitis E virus antigenic epitope and its application
  • A porcine hepatitis E virus antigenic epitope and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Porcine hepatitis E virus surface antigen

[0041] The computer software is used to screen the recognition epitopes from the corresponding proteins of the open reading frames ORF1, ORF2 and ORF3 of the whole genome of porcine hepatitis E virus. Epitope recognition is mainly based on the hydrophilicity, surface degree, flexibility of protein or polypeptide amino acid sequence, Chou-Fasman's helix, sheet and turn, Robson-Garnier's secondary structure and C- and N-terminus to predict Antigen comprehensive data (Antigenic Index, AI). Using existing software, such as: GCG (Madison, Wisconsin, USA) and DNAStar (Madison, Wisconsin, USA) to calculate AI and auxiliary recognition antigen regions from ORF1, ORF2 and ORF3 proteins of pig HEV. According to the amino acid sequences obtained from the known proteins of ORF1, ORF2 and ORF3 of porcine HEV, computer programs were used to estimate their antigenic regions (AI) (generally 10 to 15 amino acids).

[0042] After an...

Embodiment 2

[0107] Example 2 Screening of Porcine Hepatitis E Virus Epitopes

[0108] Firstly, the pig HEV total antibody ELISA kit produced by Beijing Wantai Industrial Biomedicine Co., Ltd. was used to identify the collected pig serum, and screened out pig HEV positive serum and negative serum.

[0109] The porcine HEV polypeptide obtained in Example 1 was identified by ELISA with porcine HEV positive serum and negative serum, and porcine HEV epitope antigen was screened out. The specific steps are as follows:

[0110] 1. Antigen Dilution

[0111] Take 10 μl of the peptide with a concentration of 10 mg / ml (dissolved 5 mg of the synthesized peptide in 0.5 ml of dimethyl sulfoxide (DMSO)) and mix it with 90 ul of PBS to obtain an antigen dilution solution with a solubility of 1 μg / μl. Then take 15 μl of the diluted antigen solution and mix with 5 ml of the coating solution to obtain a diluted antigen solubility of 3 μg / ml. Add antigen dilution to 100 μl / well on the microtiter plate, an...

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Abstract

The invention discloses a swine hepatitis E virus antigen epitope and applications thereof, and the sequence of the swine hepatitis E virus antigen epitope is shown in SEQ ID No:8. The swine hepatitis E virus antigen epitope obtained by computer software screening and experiment validation, on one hand, is applicable to rapid specific swine hepatitis E diagnostic kits, and can be used for rapid diagnosis of swine hepatitis E, and especially for the development of differential diagnosis technology of vaccine immune antibody and natural inapparent infection antibody for the monitoring of swine hepatitis E prevalence; on the other hand, can also be used to design specific, safe and high-efficient swine hepatitis E vaccines for the prevention of swine hepatitis E, the control of swine hepatitis E prevalence, and the obstruction of hepatitis E spreading from swine to human body.

Description

[0001] This application is a divisional application of an invention patent application with an application date of April 1, 2009, an application number of 200910048702.6, and an invention title of "Porcine Hepatitis E Virus Antigen Epitope and Its Application". technical field [0002] The invention relates to a hepatitis virus antigen epitope, in particular to a porcine hepatitis E virus antigen epitope and its application in vitro detection and vaccine. Background technique [0003] Hepatitis E virus (HEV) is a non-A-non-B hepatitis virus transmitted by fecal and oral routes. The virus can cause outbreaks in the population, mainly affecting young adults, and can cause a 20% fatality rate for pregnant women. From 1986 to 1988, Hepatitis E was prevalent in southern Xinjiang due to drinking water pollution, with a total of 119,280 cases and 707 deaths. It was the largest Hepatitis E epidemic in the world so far. In recent years, the incidence of hepatitis E has been increasi...

Claims

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Application Information

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IPC IPC(8): C07K14/08G01N33/576A61K39/29A61P31/14
Inventor 赵凯刘启文李震于瑞嵩唐雪明朱宏谭芙蓉吴萧王金斌
Owner SHANGHAI ACAD OF AGRI SCI
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