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Star anise extract for killing Meloidogyne spp. and preparation method thereof
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A technology of star anise and nematodes, applied in the field of biology
Inactive Publication Date: 2013-05-01
BEIJING UNIV OF AGRI
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In the application of phytomedicine, there are only a small number of research reports on the control of stored grain pests and agricultural pests Plutella xylostella, and there is no report on the control of plant root-knot nematodes
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Embodiment 1
[0026] Embodiment 1, the acquisition of anhydrous ethanol extract of star anise
[0027] High-speed pulverizer, type 6202, manufactured by Beijing Huanya Tianyuan Machinery Technology Co., Ltd.; desktop three-frequency digital control ultrasonic cleaner, type KQ-500VDV, Kunshan Ultrasonic Instrument Co., Ltd.; rotary evaporator, type RE-2000, Shanghai Yarong Biochemical Co., Ltd. Instrument factory; stereo microscope, Motic SMZ-140, Motic Industrial Group Co., Ltd.; micro-adjustable pipette, GILSON, France; 96-well disposable cell culture plate, JET BIOFIL type, Canada; electric constant temperature incubator, SKP-02.420 type, Huangshi Hengfeng Medical Instrument Co., Ltd.; absolute ethanol (analytical grade), Beijing Chemical Plant;
[0028] The star anise (Illicium verum) used for the test was purchased from Beijing Tongrentang Pharmacy. It belongs to Magnoliaceae and uses its fruit.
[0029] For the test root-knot nematode (Meloidogyne spp.), the diseased root of tomato r...
[0045] The contact method is used for determination, and the specific steps are: take a 96-well cell culture plate, add 150 μL of the plant extract solution obtained by the method 1 of step 2 of Example 1 with a concentration of 20 mg / mL into each well, and then add an equal volume of Mix the root-knot nematode second-instar larvae suspension obtained in step 1, let it stand at 25°C, observe under a stereomicroscope after 12h, 24h and 48h respectively, and check whether the nematodes are dead or alive (needle contact method), stiff or curled. The worms were regarded as dead, and the corrected death rate of nematodes was calculated according to the formula. With sterile water as the control, each treatment was repeated 3 times, and the results were average ± standard error.
[0046]
[0047]
[0048]Data processing and analysis: The experimental data was analyzed and sorted by DPS2000, and the results were analyzed b...
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Abstract
The invention discloses a star anise extract for killing Meloidogyne spp. and a preparation method thereof, and provides a method for preparing a star anise absolute ethanol extract. The method comprises the following steps of: 1) uniformly mixing star anise and absolute ethanol to obtain a mixture; and 2) performing ultrasonic treatment on the mixture obtained in step 1), and suction-filtering and concentrating to obtain the star anise absolute ethanol extract. Through the experiment, star anise is extracted by using absolute ethanol, the poisoning effect of extracting solution on the Meloidogyne spp. is tested, and the result is that the extract has strong poisoning activity on the Meloidogyne spp.; therefore, the extract lays a foundation for the development and application of Meloidogyne spp. biocontrol preparations in the future. In the method, an active substance for killing the Meloidogyne spp. is extracted by using a natural harmless plant material, so that the pollution caused by chemical pesticides can be reduced; and the extraction method is simple, and can be easily popularized and applied to the control of plant rootknot nematodes.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to an anise nematode extract and a preparation method thereof. Background technique [0002] Root-knot nematodes (Meloidogyne spp.) are obligate parasites on plant roots and are an important pathogen affecting agricultural production. [0003] Since the 1980s, the research on the use of plant extracts to control nematodes has gradually attracted people's attention. Up to now, there are about 102 families, 226 genera, and 316 species of nematode-killing plants, among which Asteraceae, Meliaceae, Bean family, Brassicaceae, Solanaceae, Poaceae, Euphorbiaceae, Poppyaceae, Apocynaceae, Mimosaceae, Liliaceae, etc., but the plant resources in nature are extremely rich, and many plants with nematicidal effects have not been discovered , the book "Poisonous Plants in China" has listed more than 1300 kinds of poisonous plants, many of which have insecticidal activity. And many plants can be obt...
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