A kind of preparation method and application of duck foot veneer extract
A technology of duck foot veneer and extract, which is applied in the field of research and development of pharmaceutical products, can solve the problems that the research on the anti-inflammatory and analgesic effects of duck foot veneer extract has not been reported yet, achieve significant anti-inflammatory and analgesic activity, and the preparation method is simple and convenient. Appropriate for promoting the effect of the application
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Embodiment 1
[0022] Example 1 Preparation of duck foot vetch extract
[0023] (1) Take the bark powder of Schefflera octophylla (Lour.) Harms (the particle size of the powder is not strictly limited), and extract with 10 times the volume ratio of 95% ethanol and reflux for 3 times, each for 2 hours , Stand to filter;
[0024] (2) Combine the filtrate obtained in step (1), and recover the solvent under reduced pressure to obtain the total extract;
[0025] (3) Suspend the total extract obtained in step (2) with an appropriate amount of water (the amount of water is about 3 to 5 times the mass of the total extract), and then use petroleum ether, chloroform, ethyl acetate and n-butanol to extract each 3 times, the amount of extractant is 1 to 2 times of the aqueous solution;
[0026] (4) Combine the petroleum ether layer, chloroform layer, ethyl acetate layer and n-butanol layer extracts obtained in step (3) respectively;
[0027] (5) Combine the petroleum ether layer, the chloroform layer, the ethyl...
Embodiment 2
[0028] Example 2 Preparation of duck foot vetch extract
[0029] (1) Take the bark powder of Schefflera octophylla (Lour.) Harms, and extract 2 times with 8 times the volume ratio of 85% ethanol for reflux, 2.5 hours each time, and filter by standing;
[0030] (2) Combine the filtrate obtained in step (1), and recover the solvent under reduced pressure to obtain the total extract;
[0031] (3) Suspend the total extract obtained in step (2) with water (the amount of water is about 3 to 5 times the mass of the total extract), and then use petroleum ether, chloroform, ethyl acetate and n-butanol to extract 4 times each ; (The amount of extractant is 1 to 2 times of the aqueous solution)
[0032] (4) Combine the petroleum ether layer, chloroform layer, ethyl acetate layer and n-butanol layer extracts obtained in step (3) respectively;
[0033] (5) Combine the petroleum ether layer, the chloroform layer, the ethyl acetate layer and the n-butanol layer extracts obtained in step (4) and the a...
Embodiment 3
[0034] Example 3 Preparation of Duck Feet Wood Bark Extract
[0035] (1) Take the bark powder of Schefflera octophylla (Lour.) Harms, and use 9 times the volume ratio of 75% ethanol to reflux and extract 3 times for 2 hours each time, and then stand to filter;
[0036] (2) Combine the filtrate obtained in step (1), and recover the solvent under reduced pressure to obtain the total extract;
[0037] (3) Suspend the total extract obtained in step (2) with water (the amount of water is about 3 to 5 times the mass of the total extract), and then use petroleum ether, chloroform, ethyl acetate and n-butanol to extract 4 times each ; (The amount of extractant is 1 to 2 times of the aqueous solution)
[0038] (4) Combine the petroleum ether layer, chloroform layer, ethyl acetate layer and n-butanol layer extracts obtained in step (3) respectively;
[0039] (5) Combine the petroleum ether layer, the chloroform layer, the ethyl acetate layer and the n-butanol layer extracts obtained in step (4) ...
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