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Application of curcumin in preparation of thyroid carcinoma therapeutic agent

A technology for thyroid cancer and thyroid cancer cells, which is applied in the medical field of curcumin, can solve the problem that curcumin has no report on the effect of thyroid cancer cells, and achieve a good intervention effect and the effect of promoting apoptosis

Inactive Publication Date: 2012-01-18
JIANGSU INST OF NUCLEAR MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it has been reported in the literature that curcumin has a killing effect on a variety of tumor cells, but there is no report on the effect of curcumin on thyroid cancer cells at home and abroad

Method used

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  • Application of curcumin in preparation of thyroid carcinoma therapeutic agent
  • Application of curcumin in preparation of thyroid carcinoma therapeutic agent
  • Application of curcumin in preparation of thyroid carcinoma therapeutic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Curcumin inhibits the proliferation of primary culture or thyroid cancer cell lines.

[0029] 1. Experimental materials

[0030]The experimental methods and reagents used in the following examples are conventional methods and reagents unless otherwise specified.

[0031] 1.1 Cell lines: The papillary carcinoma cell line K1, the follicular carcinoma cell line FTC-133, and the undifferentiated carcinoma cell line 8505C used in this experiment were all purchased from the European Cell Bank. FTC-133 cells were cultured with DMEM and Hams F-12 medium 1:1 (v / v); K1 cells were cultured with DMEM medium, Hams F-12 medium and MCDB105 medium 2:1:1 (v / v) For culture, 8505C cells were cultured using MEM medium. The above three cell lines were cultured in the corresponding medium supplemented with 10% fetal bovine serum (Hangzhou Sijiqing).

[0032] 1.2 Primary cell culture process: Take an appropriate amount of thyroid cancer tissue, paracancerous or contralateral nor...

Embodiment 2

[0038] Example 2: Growth inhibitory effect of curcumin on primary culture or thyroid cancer cell lines.

[0039] 1. Experimental materials

[0040] AnnexinV-FITC (fluorescein isothiocyanate-labeled phospholipid-binding protein 5) cell apoptosis detection kit, BD Pharmingen, Cat. No. 556547.

[0041] 2. Experimental method

[0042] Apoptosis detection was performed according to the instructions of the AnnexinV-FITC Apoptosis Detection Kit from BD Company. Cells were resuspended in 1× binding buffer and adjusted to 1×10 6 / mL, add 5 μL of Annexin V-FITC and 5 μL of PI solution to every 100 μL of cell suspension, and store at room temperature in the dark for 15 minutes. Add 400 μL of 1× Binding Buffer to each tube prior to flow cytometry analysis. Flow cytometry, FL1 and FL2 channels were used to detect AnnexinV and PI positive cells, and statistically analyze the cell apoptosis rate.

[0043] 3. Experimental results

[0044] Curcumin reacted with K1 or FTC-133 cells for 24...

Embodiment 3

[0045] Example 3: Curcumin promotes the cleavage of PARP in thyroid cancer cells and inhibits the expression of anti-apoptotic protein Bcl-2.

[0046] 1. Experimental materials

[0047] PARP primary antibody, Beyotime Biotechnology Company, 1:1000 dilution; Bcl-2 primary antibody, Beyotime Biotechnology Company, 1:1000 dilution; horseradish peroxidase-labeled goat anti-rabbit secondary antibody, Beyotime Biotechnology Company , 1:1000 dilution. ECL chemiluminescence kit, Biyuntian Biotechnology Company.

[0048] 2. Experimental method

[0049] The expression of apoptosis proteins in cells was detected by Western blot. K1 and FTC-133 cells in 4×10 5 Cells / mL were inoculated into 6-well cell culture plates, 1 mL per well, 37°C, 5% CO 2 After culturing overnight, add different concentrations of curcumin (10-50 μM) or solvent control group treatment, collect cells after culture, centrifuge at 6000 rpm for 5 minutes, wash twice with PBS, add 25 μL cell lysis buffer [150 mM NaC...

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Abstract

The invention discloses an application of curcumin in the preparation of a thyroid carcinoma therapeutic agent, and belongs to the technical field of curcumin pharmaceutical application. Curcumin can kill thyroid carcinoma cells specifically and has little influence on normal thyroid cells. Curcumin can promote the apoptosis of thyroid carcinoma cells, initiate the digestion of poly adenosine diphosphate-ribose polymerase (PARP), and significantly inhibit the expression of anti-apoptotic protein Bcl-2; meanwhile, curcumin can also inhibit the secretion of thyroid carcinoma cell matrix metalloproteinase-9 and the adhesion to extracellular matrix. Above results show that curcumin has good intervention effect on various links of proliferation, migration, and adhesion of thyroid carcinoma cells, and is applicable to the preparation of medicaments for treating thyroid carcinoma.

Description

technical field [0001] The invention relates to a medicine for treating thyroid cancer, specifically the application of curcumin and its derivatives in the preparation of therapeutic agents for thyroid cancer, and belongs to the technical field of curcumin's medical application. Background technique [0002] Thyroid cancer is the most common tumor of the endocrine system. It is composed of several cancers with different biological behaviors and different pathological types, mainly including papillary thyroid carcinoma (PTC) and follicular thyroid carcinoma (FTC). ), undifferentiated thyroid carcinoma (uDTC), and medulla thyroid carcinoma (MTC)). [0003] The incidence of thyroid cancer is 2-10 / 100,000, accounting for about 1% of systemic malignant tumors. According to statistics from the International Cancer Society, the incidence of thyroid cancer in various countries is increasing year by year. In 2009, the number of new cases of thyroid cancer in the United States reach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P35/00A61P35/04
Inventor 张莉俞惠新包建东宋菲张弛羽谭成
Owner JIANGSU INST OF NUCLEAR MEDICINE
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