Application of berberine as herbicide
A herbicide and berberine technology, applied in the field of pesticide research and development, can solve problems such as no herbicidal activity, and achieve the effects of good herbicidal effect, wide source and remarkable herbicidal effect.
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Embodiment 1
[0022] First prepare berberine to a certain concentration (see Table 1) of the mother liquor, then dilute it with dechlorinated tap water, use the small cup method to measure the herbicidal activity of berberine on aquatic weeds such as scallops and duckweeds, and investigate the plant The results of root growth, leaves and whole plant death are shown in Table 1 (glyphosate is the positive control drug, and CK is the blank control). The concentration range of berberine is 12.5-200 μg / mL for Echinacea, and 0.625-10 μg / mL for duckweed. Table 1 selects several points in the above two ranges value for comparison.
[0023] Table 1 Biological activity of berberine on aquatic weeds Sorrel (2-3 leaf stage) and duckweed (10d)
[0024]
[0025] Note: The concentration of glyphosate used in the field (calculated as 400 g of 10% water solution can be mixed with 20 kg of water) is: 2000 μg / mL; the values in the same column in the table are followed by the same lowercase letters, indi...
Embodiment 2
[0028] In order to further study the biological activity of berberine on other weeds, the toxicity regression equation of berberine to several common weeds was determined according to the operation steps of the toxicity regression equation, and its inhibition on root fresh weight was calculated. Medium concentration, the results are shown in Table 2 and Table 3.
[0029] Table 2 Inhibitory effect of berberine on roots of several weed seeds 7 days after treatment (pre-emergence treatment)
[0030]
[0031] Table 3 Biological activity of berberine on aquatic weed Sorrel (2-3 leaf stage) (7d)
[0032]
[0033] The results of Table 2 and Table 3 show that from the comparison of several weeds measured, berberine has the most significant activity on Arabidopsis, and the inhibitory concentration in the 7 days after the treatment is only 0.31 μg / mL; The inhibitory concentrations of hemp, ghost needle grass and green weed were only 7.65μg / mL, 7.90μg / mL and 8.14μg / mL, respectivel...
Embodiment 3
[0035] Using berberine and commercially available herbicides for mixing, the herbicidal effects of single agent and mixed agent on Herbicide were determined, and the results are shown in Table 4. The results showed that the herbicidal activity of berberine and glyphosate (the original drug with a content of 95%, purchased from the market) was significantly improved after being mixed with glyphosate, which was significantly different from the effect of a single agent, showing an obvious Synergy.
[0036] Table 4 The herbicidal effect of berberine and glyphosate on the herbicide (7d)
[0037]
[0038] Note: Values in the same column in the table are followed by the same lowercase letters, indicating no significant difference at the 0.05 level (DMRT).
[0039] *: The dosage ratio in the table refers to the mass ratio.
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