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Application of epigallocatechin gallate

A technology of food and action, applied in the field of pharmaceuticals, can solve problems such as toxicity and limited clinical application

Inactive Publication Date: 2012-03-14
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because NOTCH signaling is extremely important for the growth of the body, the artificially developed NOTCH signaling inhibitors have strong toxicity to organs with fast cell turnover such as the intestinal tract, which limits their clinical application

Method used

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  • Application of epigallocatechin gallate
  • Application of epigallocatechin gallate
  • Application of epigallocatechin gallate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] ——The effect of EGCG on the NOTCH receptor on the surface of Jurkat cell membrane

[0043] Jurkat is a human acute lymphoma (a type of leukemia) cell line capable of subculture. Jurkat cells were cultured in RPMI 1640 medium (GIBCO) containing 10% fetal bovine serum (Hyclone) and 1% penicillin and streptomycin. EGCG was dissolved in serum-free RPMI 1640 medium at a concentration of 20 mg / ml.

[0044] After the passaged Jurkat cells were treated with serum-free medium for 24 hours, EGCG was added to different final concentrations, and an equal volume of serum-free medium was added as a control, and 20 million (2*10 7 ) cells for the experiment. Process for 30 minutes. Then, the cells were collected by centrifugation at 1200 rpm for 5 minutes, and the cells were lysed with the whole cell lysate, the total protein of the cells was collected, and the protein concentration was determined.

[0045] The cell lysate was separated by 10% sodium dodecyl sulfate-polyacrylamide...

Embodiment 2

[0047] Take one unit of freshly isolated concentrated human peripheral blood lymphocytes (PBMC). After diluting 4 times with serum-free MI1640 medium, separate plasma, white blood cells and red blood cells with human lymphocyte separation medium. The specific method is: add 20 ml of human lymphocyte separation medium to a disposable 50 ml sterile pyrogen-free centrifuge tube, and carefully add diluted concentrated human peripheral blood lymphocytes on the liquid surface of the human lymphocyte separation medium 25 ml, to avoid disturbance of the interface of the two liquids to form a clear interface. Centrifuge in a low speed centrifuge at 2200 rpm for 25 minutes at room temperature (approximately 850 g). The brake function of the centrifuge is not used to make it slow down naturally and slowly to a stop. Use a sterile pyrogen-free disposable pipette to suck out the upper layer of light yellow plasma, mark it, and use it as autologous plasma for white blood cell culture. As...

Embodiment 3

[0051] After the cells were cultured with RPMI1640 medium containing EGCG (EGCG at a concentration of 0.1-100 ug / ml) and 5% autologous serum for 5-120 minutes, the cells were collected by centrifugation. Total RNA was extracted using an RNA extraction kit (Qgen Inc.), and transcribed into complementary DNA (cDNA) using a reverse transcription kit (TAKARA Inc.). Use the primer sequences listed in the sequence table to perform Real-time PCR to detect the expression of NOTCH target genes. It manifests as repression of expression of notch target genes. see results Figure 4 .

[0052] The results showed that EGCG could reduce the expression of NOTCH target genes, suggesting that NOTCH signaling was inhibited.

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PUM

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Abstract

The invention discloses the effect target of a medicine for treating tumors and indications thereof. The medicine is extracted from tea leaves of which the safety is confirmed by eating for thousands of years. The medicine has a definite chemical structure, namely epigallocatechin gallate with the English name of (-)-Epigallocatechin gallate, EGCG for short. The research of the invention shows that the effect target of the medicine and a preparation thereof in cells is the NOTCH protein of people or animals. The medicine and the preparation thereof have the effect mechanism of suppressing the abnormal activation of an NOTCH signal. The medicine adapts to the treatment of tumors with abnormal NOTCH signals. Because the abnormity of the NOTCH signals nearly occurs in all tumor cells and the medicine and the preparation thereof also have higher safe dose, the medicine and the preparation thereof have extensive and definite market application prospect.

Description

technical field [0001] The present invention relates to the use of epigallocatechin gallate, in particular to the use in the field of pharmacy. Background technique [0002] Tea is one of the most popular beverages in the world. It has various effects such as anti-oxidation, anti-aging, antibacterial and anti-virus, anti-tumor, and reducing the incidence of certain diseases. It has definite effects in reducing blood lipids, preventing and treating tumors and AIDS, and the health care function of tea has attracted widespread attention. However, its specific mechanism of action has always been considered to be very complicated and not clear, and the target of action has been unclear, which limits the application of tea in health care and drug development. [0003] The composition of tea is very complex. There are more than 500 compounds that have been isolated and identified in tea, including more than 450 organic compounds and dozens of inorganic nutrients. In the water ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P35/00A23L1/30
CPCA61K31/535A61K36/82A61P35/00
Inventor 盛军郝淑美王宣军宋爽王涵于海双李春磊李冬青王腾飞黄业伟方崇业师思严亮向泽敏杜晓翠陆洋
Owner YUNNAN AGRICULTURAL UNIVERSITY
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