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Quantum dot-based plant hormone salicylic acid acceptor probe, its preparation method and application

A plant hormone, p-aminosalicylic acid technology, applied in chemical instruments and methods, material stimulation analysis, luminescent materials, etc., can solve the problem of no substantial breakthrough in the mechanism of plant hormone action, lack of measurable signals, complex composition of plant systems, etc. problems, to achieve the effect of stable luminous performance, low equipment requirements, and low operating costs

Active Publication Date: 2013-07-24
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Due to the complex composition of the plant system and the lack of measurable signals, there has been no substantial breakthrough in the research on the mechanism of plant hormone action

Method used

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  • Quantum dot-based plant hormone salicylic acid acceptor probe, its preparation method and application
  • Quantum dot-based plant hormone salicylic acid acceptor probe, its preparation method and application
  • Quantum dot-based plant hormone salicylic acid acceptor probe, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Preparation of NaHSe: Add 1 mL of double-distilled water, 0.0265 g (7.0×10 -4 mol)NaBH 4 (It is easy to absorb moisture, and the action should be fast when weighing), and shake the vial gently to make it completely dissolved, and then add 0.0254g (3.2×10 -4 mol) Se powder. Shake the vial until all the reactants turn from black to white, cover the stopper, place it on a magnetic stirrer, and react at a constant temperature at 4°C for 1-2 hours to obtain a NaHSe solution with a concentration of 0.32mol / L.

[0050] (2) Preparation of CdSe / MPA: Add 45mL of double-distilled water into a 100mL round bottom flask, pass nitrogen to remove oxygen, add 5mL of 7.0×10 -3 mol / L CdCl 2 Solution (3.5×10 -5 mol), add 7.6μL (density 1.2199g / mL) 3-mercaptopropionic acid (8.75×10 -5 mol), use 1mol / L NaOH to adjust the pH value of the solution to 10.0, continue to stir and blow nitrogen for 30min, then add 55μL of newly prepared 0.32mol / L NaHSe solution (1.76×10 -5 mol), refluxed...

Embodiment 2

[0057] (1) Preparation of NaHSe: same as in Example 1 step (1).

[0058] (2) Preparation of CdSe / MPA: same as step (2) of Example 1.

[0059] (3) Preparation of phytohormone salicylic acid receptor probe based on quantum dots: pipette 40ml of purified CdSe / MPA quantum dot solution (the amount of Se is 1.4×10 -5 mol), add newly prepared 228μL 0.1mol / L (2.28×10 -5 mol) carbodiimide (EDC), stirred slowly, activated at 30°C for 10min, then added 3.0ml 0.01mol / L (3.0×10 -5 mol) p-aminosalicylic acid, the molar ratio of coupling agent / salicylic acid is controlled to be 3:4, and the reaction is stirred slowly at 30°C for 3h. After the reaction, put it in the refrigerator at 5° C. overnight to terminate the reaction, and obtain the quantum dot-based plant hormone salicylic acid receptor probe.

[0060] The CdSe / MPA quantum dots prepared in step (2) and the CdSe / MPA / SA obtained in step (3) were detected by transmission electron microscopy. The results showed that both CdSe / MPA and C...

Embodiment 3

[0062] (1) Preparation of NaHSe: same as in Example 1 step (1).

[0063] (2) Preparation of CdSe / MPA: same as step (2) of Example 1.

[0064] (3) Preparation of phytohormone salicylic acid receptor probe based on quantum dots: pipette 40ml of purified CdSe / MPA quantum dot solution (the amount of Se is 1.4×10 -5 mol), add newly prepared 228μL 0.1mol / L (2.28×10 -5 mol) carbodiimide (EDC), stirred slowly, activated at 40°C for 5min, then added 6.0ml 0.01mol / L (6.0×10 -5 mol) p-aminosalicylic acid, the molar ratio of coupling agent / salicylic acid is controlled to be 3 / 8, and the reaction is stirred slowly at 40°C for 4h. After the reaction was completed, the reaction was terminated overnight in a refrigerator at 5° C. to obtain a quantum dot-based phytohormone salicylic acid receptor probe.

[0065] The CdSe / MPA quantum dots prepared in step (2) and the CdSe / MPA / SA obtained in step (3) were detected by transmission electron microscopy. The results showed that both CdSe / MPA and ...

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Abstract

The invention discloses a quantum dot-based plant hormone salicylic acid acceptor probe, its preparation method and an application. Salicylic acid is modified to the surface of the quantum dot by the method of coupling to obtain the quantum dot-based plant hormone salicylic acid acceptor probe. Based on the luminescence properties of the quantum dot, salicylic acid is introduced onto the surface of the quantum dot to obtain a specific salicylic acid acceptor recognizing probe, which can be used to study the regulation mechanism of stress-tolerant hormone salicylic acid to plants. According tothe invention, CdSe / MPA quantum dot is prepared and the quantum dot-based plant hormone salicylic acid acceptor probe CdSe / MPA / SA by coupling CdSe / MPA quantum dot and salicylic acid SA is confirmed. The probe can mark the salicylic acid acceptor in plants and can pass through plant cell wall in the presence of a surface active agent. The invention is beneficial to further researches of the stress-tolerant plant hormone salicylic acid acceptor.

Description

technical field [0001] The invention belongs to the field of preparation of nanometer probes, in particular to a quantum dot-based plant hormone salicylic acid receptor probe and its preparation method and application. Background technique [0002] Stress-resistant phytohormones can improve plant resistance and tolerance to adversity. Therefore, stress-resistant phytohormones are of great significance for increasing crop yield, controlling plant growth, and improving plant cold resistance, drought resistance and disease resistance. [0003] Salicylic acid (SA for short) is a typical stress-resistant plant hormone, but the stress-resistant mechanism of salicylic acid is how plants transfer the external stimulus signal of salicylic acid into cells, and finally lead to specific physiological and biochemical changes. The mechanism is still unclear. It is generally believed that the signal transduction of salicylic acid must be combined with the receptor on the cell membrane, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88G01N21/63
Inventor 俞英刘婧文刘芳菲王伟杰
Owner SOUTH CHINA NORMAL UNIVERSITY
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