Method for extracting total saponins of cochinchinese asparagus root by using ultrasonic waves

An ultrasonic, total saponin technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, antidote, etc., can solve the problems of low extraction rate, loss of extraction of effective substances of asparagus, easy to cause pollution, etc., and achieve economical improvement. Benefit, reduction of auxiliary substances and operation steps, effect of improving extraction rate

Inactive Publication Date: 2012-05-02
重庆市秀山红星中药材开发有限公司
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pharmacological studies have shown that total aspartame saponins have the effects of regulating blood sugar, anti-tumor, anti-oxidation, anti-free radicals, and regulating immunity, so the market has a great demand for total aspartame saponins; Or heating and reflux method, one of the biggest problems in these methods is that the extraction rate is not high, there are too many auxiliary substances in the processing process, which is easy to cause pollution, and if there is high temperature, it is easy to cause damage to the active

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting total saponins of cochinchinese asparagus root by using ultrasonic waves
  • Method for extracting total saponins of cochinchinese asparagus root by using ultrasonic waves

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1.

[0020] Take 100kg of asparagus that has passed the inspection, wash and dry; put it into a pulverizer to crush, pass through a 0.5mm sieve; add 2000kg of 80% ethanol to the powder and soak for 50 minutes; then use ultrasonic waves with a frequency of 30kHz at 60°C for 25 minutes Then filter again; then repeat the immersion and ultrasonic extraction again; combine the two filtrates, and concentrate them on a rotary evaporator to 150kg under reduced pressure; then recover the ethanol in the filtrate, then extract and degrease with ether, and then extract with saturated n-butanol 4 times, add 3 times the amount of saturated n-butanol for the first time, add 2 times the amount of saturated n-butanol for each of the last three times; finally recover the saturated n-butanol under reduced pressure, concentrate the extract, and then obtain 3.275 kg of total asparagine saponins.

[0021] After inspection, the extraction rate of total asparagine saponins is 3.275%, a...

Example Embodiment

[0022] Example 2.

[0023] Take 100kg of asparagus that has passed the inspection, wash and dry it; put it into a pulverizer to crush it, pass through a 0.5mm sieve; add 2100kg of 80% ethanol to the powder and soak it for 55 minutes; Then filter again; then repeat the immersion and ultrasonic extraction again; combine the two filtrates, and concentrate them on a rotary evaporator to 150kg under reduced pressure; then recover the ethanol in the filtrate, then extract and degrease with ether, and then extract with saturated n-butanol 4 times, add 3 times the amount of saturated n-butanol for the first time, add 2 times the amount of saturated n-butanol for each of the last three times; then recover the saturated n-butanol under reduced pressure, concentrate the extract, and finally obtain 3.371 kg of total asparagine saponins.

[0024] After testing, the extraction rate of total asparagine saponins is 3.371%, and the content of total asparagine saponins in the extract is 90.3%. ...

Example Embodiment

[0025] Example 3.

[0026] Take 100kg of asparagus that has passed the inspection, wash and dry it; put it into a pulverizer to crush it, and pass it through a 0.5mm sieve; add 2200kg of 80% ethanol to the powder and soak it for 60 minutes; Then filter again; then repeat the immersion and ultrasonic extraction again; combine the two filtrates, and concentrate them on a rotary evaporator to 150kg under reduced pressure; then recover the ethanol in the filtrate, then extract and degrease with ether, and then extract with saturated n-butanol 4 times, add 3 times the amount of saturated n-butanol for the first time, add 2 times the amount of saturated n-butanol for each of the last three times; then recover the saturated n-butanol under reduced pressure, concentrate the extract, and finally obtain 3.331 kg of total asparagine saponins.

[0027] After inspection, the extraction rate of total asparagine saponins is 3.331%, and the content of total asparagine saponins in the extract ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting total saponins of cochinchinese asparagus root by using ultrasonic waves, which comprises the steps of cleaning, grinding, soaking, performing ultrasonic extraction, repeating, concentrating, degreasing, extracting, and concentrating, and is characterized by comprising the following specific steps of: adding 80 percent ethanol in an amount which is 20 to 22 times that of powder into the powder, soaking for 50 to 60 minutes, extracting at the temperature of between 60 and 63 DEG C for 25 minutes by using the ultrasonic waves with the frequency of 30 to 35kHz, filtering, repeating once, concentrating under reduced pressure until the concentration is 20 times the original concentration, recovering the ethanol, degreasing, extracting for four times by using saturated n-butanol, recovering the saturated n-butanol, and concentrating under reduced pressure to obtain the total saponins of the cochinchinese asparagus root. The method has the advantages that: by ultrasonic wave extraction, beneficial substances in the cochinchinese asparagus root can be fully precipitated, auxiliary substances and operation steps are reduced, the extraction rate of the total saponins of the cochinchinese asparagus root is improved, and economic benefits are improved.

Description

technical field [0001] The invention relates to a method for extracting total asparagine saponins, in particular to a method for ultrasonically extracting total asparagine saponins. Background technique [0002] Asparagus cochinchinensis (Lour.) Merr., also known as asparagus, is an evergreen vine of Liliaceae, and its roots are used as medicine. It is mainly produced in Guizhou, Guangxi, Yunnan, Gansu, Anhui, Henan, Hunan, Hubei, Sichuan and Jiangxi. Asparagus was first recorded in "Shen Nong's Materia Medica", and it has been recorded in successive dynasties of herbal medicines. It is sweet, bitter, cold in nature, belongs to the lung and kidney meridian, can nourish yin and moisten dryness, clear lung and generate body fluid; it is mainly used for dry cough due to dryness of the lung, cough due to consumptive fatigue, thirsty body wound, insomnia due to complex heart, internal heat and thirst, constipation due to dryness of the intestine, etc. disease. It can also trea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/8965A61P3/10A61P35/00A61P37/02A61P39/06
Inventor 祝利赵勇彪杨再江向廷军何全慧
Owner 重庆市秀山红星中药材开发有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap