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Day lily flower and method for detecting day lily flower preparation

A detection method, the technology of Hemerocallis flower, applied in the direction of measuring device, color/spectral characteristic measurement, instrument, etc., can solve problems such as non-compliance, affecting product quality and curative effect, and unfavorable production efficiency of drug standardization

Active Publication Date: 2015-05-20
倪健
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have shown that Hemerocallis flower has obvious antidepressant effect, and the preparation made from Hemerocallis flower extract can effectively treat depression, but there are still few researches on the quality inspection of Hemerocallis flower preparation, which will affect the quality and curative effect of the product. It is not conducive to the standardized production of medicines and the improvement of production efficiency, and it is not in line with the development trend of modern Chinese medicine

Method used

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  • Day lily flower and method for detecting day lily flower preparation
  • Day lily flower and method for detecting day lily flower preparation
  • Day lily flower and method for detecting day lily flower preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0086] Experimental example 1 TLC identification experiment

[0087] 1 Instruments and materials

[0088] 1.1 Instruments TU-1810 UV-Vis Spectrophotometer (Beijing General Instrument Co., Ltd.); SD-20 Shimadzu Liquid Chromatograph (Japan), Quaternary Pump (LC-20ATvp), SPD-M20A Diode Array Detector, LC-liquid chromatography workstation. Sartorius electronic analytical balance (BT 125D). KQ3200DE Ultrasonic Apparatus (Kunshan Ultrasonic Instrument Co., Ltd.).

[0089] 1.2 Material preparation samples were prepared by Beijing University of Traditional Chinese Medicine (same as Example 1); rutin reference substance was purchased from China Institute for Food and Drug Control (100080-200707), methanol was chromatographically pure (Fisher Company); water was Watsons purified water; All other reagents were analytically pure.

[0090] 2 identification

[0091]Get 0.5 g of the preparation (prepared by Example 1), dissolve it ultrasonically with an appropriate amount of 30% ethanol...

experiment example 2

[0094] The total flavone content determination experiment of experimental example 2 ultraviolet spectrophotometry

[0095] 1 Selection of measurement wavelength

[0096] After the rutin standard solution and Hemerocallis extract were color developed according to the experimental method, they were scanned in the wavelength range of 400-600nm. As a result, the rutin standard solution had the maximum absorption at 510nm after the color development, and the Hemerocallis extract also had color development at this wavelength. Absorption, the experiment chooses 510nm as the measuring wavelength.

[0097] 2 Preparation of the test solution

[0098] Accurately weigh 20mg of the preparation (prepared by Example 1), put it in a 10mL measuring bottle, add 30% ethanol to the mark, and process it ultrasonically for 10min to obtain it.

[0099] 3 Preparation of reference solution

[0100] Accurately weigh an appropriate amount of rutin reference substance, add methanol to make a solution ...

experiment example 3

[0131] The rutin content determination experiment of experimental example 3HPLC method

[0132] 1 Chromatographic conditions and system suitability test

[0133] Octadecylsilane bonded silica gel is used as filler; methanol: 0.5% glacial acetic acid solution (40:60) is used as mobile phase; the number of theoretical plates should not be less than 3000 based on the rutin peak.

[0134] 2 Selection of measurement wavelength

[0135] Get the rutin reference substance and the sample solution into the liquid phase respectively, DAD detector detection, the spectrogram of rutin shows that there are two large absorption peaks at 254nm and 365nm, but the number of peaks detected by the sample at 254nm is more, so 254nm was selected as the measurement wavelength.

[0136] 3 Preparation of reference solution

[0137] Accurately weigh an appropriate amount of rutin reference substance, add methanol to make a solution containing 0.4mg per 1mL, shake well, and obtain.

[0138] 4 Prepara...

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Abstract

The invention discloses day lily flower and a method for detecting a day lily flower preparation. The method for detecting the day lily flower preparation comprises one or more the following identification methods and / or content measurement methods of: A, thin-layer chromatography identification; B, ultraviolet spectrophotometry content measurement; C, HPLC (High Performance Liquid Chromatography) content measurement; and D, HPLC fingerprint chromatography of an original medicinal material. The invention also further provides an HPLC detection method and an HPLC fingerprint chromatography detection method of the day lily flower. According to the day lily flower and the method for detecting the day lily flower preparation, which are disclosed by the invention, the identification specificity is good and the method is low in cost and is practical and is rapid in obtaining a result; the product can be effectively subjected to quality detection by the content measurement methods; and an analysis method in the fingerprint chromatography detection is stable, reliable and effective and the basis is provided for identification and quality evaluation and control of the day lily flower preparation and the medicinal material.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine and its preparation, in particular to a detection method of Hemerocallis flower and its preparation. Background technique [0002] Hemerocallis citrina Baroni (Hemerocallis citrina Baroni), also known as "day lily" and "one day lily", commonly known as "forgetfulness grass", belongs to the perennial herbaceous plant of the genus Hemerocallis genus in the family Liliaceae. Rich in nutrients, the main chemical components are protein, fat, carbohydrates, and a variety of vitamins, alkaloids, glycosides, flavonoids, carotene, volatile oils, tannins, and inorganic minerals calcium and phosphorus, mainly flavonoids The ingredients can be used for both medicine and food. Li Shizhen of the Ming Dynasty said in "Compendium of Materia Medica" that it has the effects of calming the nerves and refreshing the brain, increasing wisdom and widening the chest, beautifying and nourishing blood,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/90G01N21/33
Inventor 倪健邹忆怀朱陵群
Owner 倪健
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