Application of prosaposin (psap) serving as effect marker in detection of male reproductive toxicity of polychlorinated biphenyl

A technology of saposin and polychlorinated biphenyls, which is applied in the fields of biological testing, microbial measurement/inspection, material inspection products, etc., can solve the problems of high cost and complicated operation procedures, and achieve the effect of small damage

Inactive Publication Date: 2014-06-11
XIAMEN UNIV
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  • Abstract
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Problems solved by technology

However, the direct use of gene chips to evaluate the toxicity of chemicals and predict the toxic effects of toxic chemicals on organisms requires high costs due to the complexity of the operating procedures.

Method used

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  • Application of prosaposin (psap) serving as effect marker in detection of male reproductive toxicity of polychlorinated biphenyl
  • Application of prosaposin (psap) serving as effect marker in detection of male reproductive toxicity of polychlorinated biphenyl
  • Application of prosaposin (psap) serving as effect marker in detection of male reproductive toxicity of polychlorinated biphenyl

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Embodiment Construction

[0033] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0034] 1. Exposure of Experimental Animals

[0035] Healthy adolescent male mice of the C57BL / 6 strain were used as experimental animals, with an average body weight of 15 g, provided by the Experimental Animal Center of Xiamen University (SPF grade). The experimental temperature was controlled at 24±1°C, the day and night cycle was 12:12h (lights were turned on at 08:00 in the morning), and the mice had free access to food and water during the experiment. Male mice were randomly divided into 12 groups. The exposure concentration was designed according to the concentration of PCBs in the environment: 0.5, 5, 50 and 500 μg / kg. Mice were intragastrically exposed to poison once every 3 days, and the intragastric dose was 5 μL per gram of body weight, and the control group was given 5 μL of normal saline per gram of body weight, and the exposure lasted ...

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Abstract

The invention discloses application of prosaposin (psap) serving as an effect marker in detection of the male reproductive toxicity of polychlorinated biphenyl, and relates to psap. The psap is multifunctional glycoprotein, has the molecular weight of 70kD, and exists in some biological fluids. The method for screening the psap comprises the following steps of: exposing a small mouse by using different quantities of polychlorinated biphenyl, and acquiring a sample; screening difference expression genes of the sample; performing function accumulation on the screened difference expression genes to obtain a difference expression gene relevant to a reproductive function; detecting and analyzing the change of the obtained difference expression gene on an mRNA transcription level and a protein expression level by using a real-time fluorescent quantitative polymerase chain reaction (PCR) technology and an immunoblotting method along with the increase of a polychlorinated biphenyl (PCB) exposed amount and the exposing time; detecting and analyzing sperm parameters; and counting relevant coefficients among the effect marker, an in-vivo polychlorinated biphenyl level and a semen index through Spearman correlation analysis.

Description

technical field [0001] The invention relates to prosaposin, in particular to the application of prosaposin as an effect marker in detecting the male reproductive toxicity of polychlorinated biphenyls. Background technique [0002] Polychlorinated Biphenyls (PCBs) are a class of synthetic organic compounds. There are 209 isomers according to the number and position of chlorine atom substitution. Due to the advantages of good chemical inertness, heat resistance, non-flammability and high dielectric constant, PCBs were used in heat medium, special lubricants, plasticizers, coatings, dustproof agents, ink additives, insecticides and Manufacture of carbon paper, etc. and as insulating oil in power equipment such as capacitors and transformers. With the continuous application of PCBs, the accumulation in the environment is increasing. Moreover, once PCBs enter the environment, they are extremely difficult to degrade and circulate in the entire ecosystem, thus causing "global en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68C12Q1/68C12Q1/06
Inventor 左正宏蔡嘉力王重刚李博文陈奕欣
Owner XIAMEN UNIV
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