Fixing device for non-invasive patch-type detection inductor for repeated use
A technology of fixtures and sensors, which is applied in the field of cell and microbial culture, can solve the problems of being unable to be reused and increasing the cost of culture, and achieve the effect of reducing costs
Active Publication Date: 2013-07-10
安赛搏(重庆)生物技术有限公司
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AI Technical Summary
Problems solved by technology
However, the current design makes the patch sensor disposable and cannot be reused. Since the price of each patch sensor reaches 20-100 euros, the cost of cultivation is greatly increased.
Method used
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Experimental program
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Effect test
Embodiment 1
[0021] Embodiment 1. The above-mentioned installed device of the present invention was repeatedly used for three consecutive times to carry out continuous dissolved oxygen monitoring of grape cell culture in a 20L scale disposable bioreactor. By monitoring and controlling the change of dissolved oxygen in the reactor online, the cells can grow normally. See image 3 , Figure 4 , Figure 5 .
Embodiment 2
[0022] Example 2. Continuous dissolved oxygen monitoring of grape cell culture in a 200L-scale disposable bioreactor using a patch sensor. By monitoring and controlling the change of dissolved oxygen in the reactor online, the cells grow normally. See Figure 6 .
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The invention discloses a fixing apparatus for a non-invasive patch-type detection inductor which can be repeatedly used. The fixing apparatus comprises an inductor bracket and a bracket fixing device. The inductor bracket comprises an inductor bracket upper piece (1), an inductor bracket lower piece (3) and a bracket locking device (4); the bracket locking device (4) is movably connected to the inductor bracket upper piece (1) and the inductor bracket lower piece (3). Inductor fixing snap-slots are respectively provided on the lower surface of the inductor bracket upper piece (1) and the upper surface of the inductor bracket lower piece (3). Holes are provided at the center of the inductor bracket upper piece (1) and the center of the inductor bracket lower piece (3). The bracket fixing device is a hollow fixing plate (6) with a connection pipe connected to the lower surface. The fixing plate is magnetic. The materials of the inductor bracket contain iron. With the device provided bythe invention, a patch-type detection inductor can be repeatedly used, such that the cost of detection inductor application is greatly reduced.
Description
technical field [0001] The invention belongs to the field of cell and microorganism culture, and more specifically relates to a non-invasive detection sensor fixing device for detecting various parameters in the process of cell and microorganism culture. Background technique [0002] All microorganisms or cell culture processes require online detection of various parameters inside the reactor, including dissolved oxygen, carbon dioxide, acidity and alkalinity, and other culture physiological and biochemical indicators. The bioreactor detection method used for traditional microorganism or cell culture is invasive detection. The detection probe is basically rod-shaped, and the detection probe is inserted into the reactor to detect various indicators. This method is applied to shake flasks and disposable biological reactions. device will be very difficult. Shake flasks and disposable bioreactors are usually made of non-metallic materials, which have poor tolerance. It is diffi...
Claims
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IPC IPC(8): C12M1/00
Inventor 龚卫东陈牛魏先宇
Owner 安赛搏(重庆)生物技术有限公司