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Aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent

A technology of superparamagnetic iron oxide and nanocomposite, which is used in drug delivery, pharmaceutical formulations, preparations for in vivo tests, etc., can solve problems such as stable dispersion of hydrophobic SPIO particles.

Inactive Publication Date: 2012-07-04
戴永强 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a single dextran alone cannot make the hydrophobic SPIO particles disperse stably in aqueous solution.

Method used

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  • Aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent
  • Aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent
  • Aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent

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Embodiment Construction

[0036] The present invention will be further described below in combination with specific embodiments.

[0037] 1: Construction of ssDNA amplification library

[0038] In the PCR system (10XPCR reaction buffer 10ul, dNTPs (25mM) 8ul, 5' primer (25pmol / ul) 2ul, 3' primer (25pmol / ul) 2ul, template 0.25ug, Pfu DNA polymerase 1ul), the downstream primer The 5' end is labeled with biotin. Using the original ssDNA library as a template for PCR amplification, a dsDNA library with biotin at the 3' end can be obtained.

[0039] The original ssDNA library has 20-25 random series and a random DNA library with a total length of 85 bases. The two ends of the library are fixed series, and primers can be designed to perform PCR amplification on the library. The series of ssDNA are:

[0040] 5'-T AATACAGACTX 25 GATCCCAGTCA-3'

[0041] After the dsDNA product is separated and purified, it is combined with streptavidin magnetic beads, the amplified dsDNA is bound t...

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Abstract

The invention provides an aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent. The aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent comprises the following components: (a) oligonucleotides or peptide aptamers for molecular recognition; (b) superparamagneticiron oxide nanoparticles; and (c) a biocompatible amphiphilic dextran block copolymer carrier, which is used for connecting the superparamagneticiron oxide nanoparticles and the oligonucleotides or peptide aptamers for molecular recognition. The aptamers, the superparamagnetic iron oxide nanoparticles and a block copolymer of polyethylene glycol, polycaprolactone and polysaccharide are assembled into a nanomicelles complex; through the targeting effect of specifically bounding the aptamers and a target molecule and magnetic resonance imaging features of SPIO nanoparticles, the complex nanomicelles is established by the biocompatible block copolymer; and after the aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent enters a body, small foci are positioned in a targeting way so as to realize early targeting positioning and diagnosis of the small foci in vitro and in vivo through the conventional magnetic resonance imaging diagnostic equipment. By selecting a proper target molecule aptamer, the aptamer target-based superparamagnetic iron oxide nanocomposite magnatic resonance imaging contrast agent can be applied to cell signaling, metabolic process, gene detection, virus detection, tumor detection and other biological researches.

Description

technical field [0001] The invention relates to the preparation of superparamagnetic iron oxide nanoparticles by screening oligonucleotides or polypeptide aptamers that can specifically bind to certain targets, and the assembly of nanocomposites through carrier block polymer micelles, which is suitable for minimal lesions Localized magnetic resonance imaging contrast agents for early magnetic resonance imaging diagnosis. Background technique [0002] Due to the current trend of aging society and the increase of people's life expectancy worldwide, people gradually realize the importance of how to achieve personal healthy life. Accordingly, the need for early diagnosis of major diseases such as cancer and other life-threatening diseases has been emphasized, and thus there has been an urgent need to develop a diagnostic technique that can be used for early diagnosis when it is considered necessary and has precise localization of minute lesions. In this context, people have bee...

Claims

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Application Information

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IPC IPC(8): A61K49/12A61K49/18
Inventor 戴永强
Owner 戴永强
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