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Dairy goat Boule gene and application of dairy goat Boule gene in promoting meiosis of male germ cells

A germ cell and meiosis technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as difficult control of germ cell meiosis, and achieve the effect of promoting meiosis

Inactive Publication Date: 2012-07-04
NORTHWEST A & F UNIV
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Problems solved by technology

[0005] Although a variety of research systems for in vitro proliferation and differentiation of germ cells have been established so far, many key genes and proteins that regulate meiosis have also been discovered in Knockout experiments, but the establishment of meiosis and spermatogenesis research in vitro has not yet been fully realized. Model, poorly controlled initiation of germ cell meiosis in vitro

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  • Dairy goat Boule gene and application of dairy goat Boule gene in promoting meiosis of male germ cells
  • Dairy goat Boule gene and application of dairy goat Boule gene in promoting meiosis of male germ cells
  • Dairy goat Boule gene and application of dairy goat Boule gene in promoting meiosis of male germ cells

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Embodiment Construction

[0034] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0035] 1. Cloning and vector construction of dairy goat Boule gene

[0036] First, total RNA was extracted from dairy goat testis tissue, and cDNA was obtained by the reverse transcription kit, which was used as a template for amplification;

[0037] According to the complete cDNA sequence of the cattle b-Boule gene (NM_001102115) in the GenBank database, primers were designed using Primer Premier 5.0 primer design software, and suitable restriction enzymes were selected to specifically design the following primers:

[0038] Upstream primer: tc gaattc ga aagtctccgt cgggaagcgt

[0039] Downstream primer: ct gtcgac gg cagcttctag ccggttcatt g

[0040] The underlined part in the upstream primer is the Ecor I restriction site, and the underlined part in the downstream primer is the SalI rest...

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Abstract

The invention discloses a dairy goat Boule gene and application of the dairy goat Boule gene in promoting meiosis of male germ cells. The CDS (coding sequence) of the gene (SEQ.ID.NO.1) is shown as SEQ.ID.NO.2. An expression vector constructed based on the dairy goat Boule gene sequence comprises a Boule gene sequence shown as SEQ.ID.NO.1, a fluorescence labeled gene GFP (green fluorescent protein) is linked downstream of the Boule gene sequence, and an antibiotic screening gene kanr / neor is linked downstream of the GFP. The dairy goat Boule gene sequence is transfected into the male germ cells to promote the meiosis of the male germ cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to germ cell meiosis-related genes, in particular to a dairy goat Boule gene and its application in promoting male germ cell meiosis. Background technique [0002] Mammalian spermatogenesis can be divided into A-type spermatogonia proliferation and B-type spermatogonia differentiation into primary spermatocytes, primary spermatocytes form round spermatids through meiosis, and spermatids transform into mature spermatids, etc. The main stages of spermatogenesis, of which meiosis is the key step in the process of spermatogenesis. Meiosis is a division method in which the number of chromosomes in germ cells is halved. During meiosis, chromosomes are copied only once, and cells divide twice in a row. Meiosis not only plays an important role in ensuring the stability of the number of chromosomes in species, but also a mechanism for species to adapt to changing environments. Boule is a new mem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/62C12N15/63C12N5/10
Inventor 李明昭华进联刘超
Owner NORTHWEST A & F UNIV
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