Method for preparing biological antibacterial organic fertilizer by using immune induced biological technology

Abstract

The invention discloses a method for preparing a biological antibacterial organic fertilizer by using an immune induced biological technology. In the method, Super-strong immune protein synthesizing capability of silkworm is utilized. The method comprises the following steps of: synthesizing a large amount of sericin antimicrobial peptides through silk glands in the middle of nature silkworm with five years old, and synthesizing silk fibroin antimicrobial peptides through silk glands at the rear; and regulating and controlling silk fibroin genes by using a biological high active agent, and synthesizing and amplifying by using a solid-phase or liquid-phase biological technology to obtain the biological antibacterial organic fertilizer. The method is simple and easily learnt in biological synthetic route technology, low in production cost and high in yield controllability; the disease preventing capability reaches above 80 to 95 percent; the fertilizer is easily converted and is convenient for industrialized production; and the biological antibacterial organic fertilizer prepared by adopting the method is mixed with soil in agricultural use, roots are soaked into the fertilizer solution before planting, and the fertilizer has a strong antibacterial effect and efficient and broad-spectrum antibacterial advantages on infection caused by withering fusarium and pathogens such as Gram-positive bacteria, Gram-negative bacteria or bacillus subtilis, bacterial wilt and the like.

Description

technical field [0001] The invention relates to a preparation method of a biological antibacterial organic fertilizer, in particular to a method for preparing a biological antibacterial organic fertilizer by immune induction biotechnology. Background technique [0002] The continuous cropping obstacles of some melons, fruits, vegetables and other crops have become the bottleneck restricting agricultural production, resulting in the waste of land resources, manpower, material resources, financial resources and other agricultural inputs, affecting agricultural economic benefits, and also greatly hitting farmers. positivity. Among the continuous cropping soil obstacles of continuous cropping crops, continuous cropping blight mainly caused by fungal pathogens (Fusarium oxysporum) is the most serious obstacle factor. More and more serious; Pepper bacterial wilt caused by R. solanacearum usually causes 20-30% of the death of peppers once it occurs, and when it is severe, it is as...

AI Technical Summary

Problems solved by technology

[0007] However, there is currently no use of immune-inducing biotechnology, using the middle silk gland of the fifth-instar silkworm to synthesize high-volume sericin antimicrobial peptides, synthesizing silk fibroin antimicrobial peptides in the posterior silk gland, and then synthesize and expand through solid-phase or liquid-phase biotechnology. Zeng, research on the preparation of biological antibacterial organic fertilizers for agricultural antibacterial production