Method by adopting molecular marker-assisted backcross to improve gibberellic disease expansion resistance of wheat

A molecular marker-assisted, scab-blight technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of time-consuming and labor-intensive resistance, hinder the effective use of resistance sources, etc., to speed up the promotion and improve the breeding. Choose efficient, efficient effects

Inactive Publication Date: 2012-07-25
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Therefore, using the traditional backcross breeding method to improve wheat scab resistance is time-consumin

Method used

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  • Method by adopting molecular marker-assisted backcross to improve gibberellic disease expansion resistance of wheat
  • Method by adopting molecular marker-assisted backcross to improve gibberellic disease expansion resistance of wheat
  • Method by adopting molecular marker-assisted backcross to improve gibberellic disease expansion resistance of wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Using SSR markers closely linked to target QTLs to assist backcrossing to improve expansion resistance of wheat lines

[0051] F 1 ; Then use Mianyang 99-323 as the reincarnation parent to backcross and get BC 1 f 1 . Will BC 1 f 1 The isolated population is planted in the field as a single plant, listed at the seedling stage, and the DNA of each individual plant is extracted, and the genotype is analyzed using the SSR markers closely linked to the target QTL, and the heterozygous individual plants carrying the target QTL are selected to continue backcrossing to obtain BC 2 f 1 . Use a similar method to select individual plants and continue backcrossing to obtain BC 3 f 1 . in bc 3 f 1 In the segregation population, the homozygous individual plants carrying the target QTL were selected according to the marker genotypes to obtain near-isogenic lines by selfing. A total of 1 generation of crossing, 3 generations of backcrossing and 2 generations of s...

Embodiment 2

[0062] Example 2 Select SSR markers throughout the wheat genome to analyze the background recovery rate

[0063] In order to speed up the restoration of recurrent parents in areas other than the target QTL, the genetic map constructed using the Nanda 2419×Wangshuibai recombinant inbred line population (Xue SL, Zhang ZZ, Lin F, Kong ZX, Cao Y, Li CJ, Yi HY, Mei MF, Zhao DM, Zhu HL, Xu HB, Wu JZ, Tian DG, Zhang CQ, Ma ZQ (2008) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181-189) selected a marker every 20-30cM, and a total of 150 pairs of SSR markers distributed on 21 chromosomes were selected for the background recovery rate analysis of each generation.

[0064] Results show: in BC 2 f 1 In this generation, 97 pairs of markers were randomly selected from 150 pairs of markers to evaluate the genetic background of 19 heterozygous individuals with Qfhs. , much higher than the 87.5% theore...

Embodiment 3

[0065] Example 3: Identification of Field Scab Resistance Expansion of Near-Isogenic Lines

[0066] Field resistance evaluation was carried out in Jiangpu experimental field and Pailou experimental field of Nanjing Agricultural University in 2010 and 2011, respectively. Random block design was adopted, with 2 rows per plot, row length 1.5m, row spacing 0.25m, plant spacing 0.1m, and 15 plants per row. In the flowering stage, the single-flower drip method was used to inoculate, and the Gibberella used was a mixture of strong pathogenic bacteria F4, F15, F17 and F34 strains, and the concentration of the spore liquid was 1×10 5 spores / ml. The number of diseased spikelets (NDS) and the length of diseased rachis (LDR) were investigated 21 days after flowering, and the spreadability of the bred near-isogenic lines against scab was evaluated.

[0067] The results showed that compared with the recurrent parents, the number of diseased spikelets and the length of the diseased axis of...

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Abstract

The invention discloses a method by adopting molecular marker-assisted backcross to improve gibberellic disease expansion resistance of wheat. A recombinant inbred line family carrying expansion-resistant quantitative trait loci (QTL) is adopted as a donor, a line which has excellent comprehensive character but is infected by gibberellic diseases is adopted as a receptor, and the donor is hybridized and backcrossed with the receptor. Two side markers which are closely interlocked with a target QTL are utilized for selecting a foreground in each backcross generation, 150 pairs of simple sequence repeats (SSR) markers which are distributed in an entire wheat genome are utilized for selecting a background respectively for a single plant carrying the target QTL, and the single plant with the highest background repeated rate is selected for the next generation of backcross. A near-isogenic line with the resistance being obviously improved and other characters being basically consistent with that of a recurrent parent is obtained after being continuously backcrossed for three generations and then being inbred for two generations. A target single plant can be obtained by extracting deoxyribonucleic acid (DNA) of seedling leaves of backcrossed generations and analyzing a marker genotype, so that the problems in phenotypic selection that the gibberellic disease resistance is susceptible to an environmental condition and a resistance phenotype can be determined only in a flowering period can be effectively solved; and meanwhile, the population size can be reduced, the breeding cost can be reduced, and the gibberellic disease expansion resistance of the wheat line can be rapidly and effectively improved.

Description

1. Technical field [0001] The invention discloses a method for quickly improving the expandability of wheat scab resistance by molecular marker-assisted backcrossing, which belongs to the field of crop molecular breeding and is specially used for the selection and identification of wheat scab resistance expansion germplasm. 2. Background technology [0002] Wheat is one of the most important food crops in the world, and its products are indispensable to human food security. Head blight caused by Fusarium graminearum Schwabe is an important disease in wheat production, which widely occurs in warm, humid and semi-humid regions. The hazards include sterility and reduced seed plumpness, resulting in a severe drop in yield, generally by 10-15%, sometimes as high as 50%. Diseased wheat grains also contain toxins (mainly deoxynivalenol, DON), which will directly endanger the health of humans and animals after consumption. Due to global warming and changes in farming systems, scab...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12Q1/68
Inventor 马正强薛树林李国强郜忠霞
Owner NANJING AGRICULTURAL UNIVERSITY
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