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Candida selection medium for stool specimens and preparation method thereof
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A technology for selecting medium and candida, which is applied in the field of candida selective medium, can solve the problems of low candida isolation rate and inability to grow candida, and achieve the effect of reliable detection and high isolation rate
Inactive Publication Date: 2013-08-28
刘艳
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Problems solved by technology
However, the fungal selection medium currently used in clinical practice is mainly used for the isolation of Candida from clinical specimens such as urine, sputum, blood, bile, and ascites, such as potato dextrose agar (PDA) medium, which has a low isolation rate for Candida in stool samples. , the reason is mostly that Escherichia coli in the feces grows faster than Candida on the current fungal culture medium, and soon covers the culture medium, and Candida cannot grow
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Embodiment 1
[0022] Example 1, the formula for preparing 1000ml medium contains: 3g of malt extract powder; 20g of beef heart powder; 1.2g of magnesium sulfate; 15g of agar; 0.02g of gentamicin sulfate; g; add distilled water to 1000ml. Preparation method: Dissolve malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, and gentamicin sulfate in distilled water, mix well, distill water to 1000ml, 121°C, extinguish for 15 minutes Pack after sterilization.
Embodiment 2
[0023] Example 2, the formula for preparing 1000ml of medium contains: 10g of malt extract powder; 10g of beef heart powder; 2g of magnesium sulfate; 20g of agar; 0.005g of gentamicin sulfate; 0.4g of aniline blue; ; Add distilled water to 1000ml. Preparation method: Dissolve malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, and gentamicin sulfate in distilled water, mix well, distill water to 1000ml, 121°C, extinguish for 15 minutes Pack after sterilization.
Embodiment 3
[0024] Example 3, the formula for preparing 1000ml medium contains: 3g of malt extract powder; 20g of beef heart powder; 1.2g of magnesium sulfate; 15g of agar; 0.02g of gentamicin sulfate; g, allithiamine 0.05g, glycocholic acid 0.1g; add distilled water to 1000ml. Preparation method: Dissolve malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, gentamicin sulfate, allithiamine, glycocholic acid in distilled water, mix well, and distilled water Dilute to 1000ml, sterilize at 121°C for 15 minutes, and dispense.
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Abstract
The invention discloses a candida selection medium for stool specimens and a preparation method of the candida selection medium. The candida selection medium for the tool specimens is characterized by comprising the following components in each 1000ml culture medium: malt extract 3-10g; beef heart for infusion 10-20g; magnesium sulfate 1.2-2g; agar 15-20g; gentamycin sulfate 0.005-0.02g; aniline blue 0.1-0.4g; calcium citrate tetrahydrate 0.01-0.2g; and water added to 1000ml. The preparation method comprises the following steps of: weighting the malt extract, beef heart for infusion, magnesium sulfate, agar, gentamycin sulfate, aniline blue, calcium citrate tetrahydrate, allithiamine, and glycocholic acid and dissolving the components into an orchis decoction; uniformly blending, drippingthe water until constant volume, and sterilizing the mixture and then sub-packaging the mixture. Compared with the prior art, the detection result of the candida from the stool specimens is reliable,and the isolation rate of the candida is high.
Description
technical field [0001] The invention relates to the field of test microorganism culture, in particular to a Candida selection medium used for stool samples. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Desired microorganisms are separated from confounding microorganisms. Candida is the most common opportunistic pathogen among fungi, also known as Candida, which often parasitizes on human skin, oral cavity, vagina and intestinal mucosa, etc. , prone to candidiasis. At present, the clinical detection method of Candida is mainly Gram staining, but 20% of Candida Gram staining cannot be detected positively. Moreover, if it is necessary to determine the type of Candida, it needs to be cultured and identified with respect to its colony morphologica...
Claims
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