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Method for prolonging preservation period of rice leafroller eggs

A technology of rice leaf roller and storage period, which is applied to the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of sharp drop in insect survival rate and hatching rate, so as to ensure economy, continuity, and prolong The effect of shelf life and improvement of utilization rate

Inactive Publication Date: 2012-08-01
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Temperature is an important factor affecting the development of insects. Generally, low temperature will slow down the development progress of insects, but the survival rate of insects or the hatching rate of eggs will also decrease sharply at low temperatures

Method used

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Embodiment Construction

[0008] The method of the present invention can be implemented in the following manner, but is not limited to this manner.

[0009] Low-temperature training conditions: put the eggs of the rice leaf roller produced at the same night at 25°C in a translucent plastic beaker with wet absorbent paper on the bottom, then cover the mouth of the beaker with gauze, and turn it at 7:30 in the morning. Into a light incubator at 23°C and kept for 2 hours; then transferred to a light incubator at 21°C for 4 hours; then transferred to a light incubator at 19°C for 8 hours; finally transferred to 17°C at 21:30 and kept for 10 hours Hours, turn to 15°C at 7:30 the next morning.

[0010] Conditions for cryopreservation: eggs after low-temperature training were placed in a light incubator at 15°C for 5 days, 10 days, 15 days, 20 days, 25 days and 30 days respectively.

[0011] Conditions for heating to activate egg hatching: After keeping at 15°C for a certain number of days, turn the low-temp...

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Abstract

The invention provides a method for prolonging the preservation period of rice leafroller eggs. The invention provides an egg preservation method which can firstly implement progressive cooling training on the rice leafroller eggs, then keep for a long time at the temperature of 15 DEG C and further perform progressive heating activation against the problem that rice leafrollers can not be kept with high efficiency for the long time during indoor rearing. The method is characterized in that the method comprises the following steps of: keeping the eggs laid at the temperature of 25 DEG C at 23 DEG C, 21 DEG C, 19 DEG C and 17 DEG C for 2 hours, 4 hours, 8 hours and 10 hours respectively, then placing at 15 DEG C, preserving for a certain number of days, further sequentially keeping the eggs at 17 DEG C, 19 DEG C, 21 DEG C and 23 DEG C for 2 hours, 4 hours, 8 hours and 10 hours respectively, and then hatching the eggs at 25 DEG C. According to the method disclosed by the invention, the eggs can be effectively preserved for 22 days, and the hatching rate is 75% of that at the normal temperature of 25 DEG C. According to the method disclosed by the invention, the flexibility in rearing of the rice leafrollers can be effectively improved, the cost can be reduced, and powerful guarantee can be provided for smooth development of test research of the rice leafrollers.

Description

technical field [0001] The invention belongs to the field of insect feeding, and in particular relates to a method for prolonging the storage life of rice leaf roller eggs. Background technique [0002] Rice leaf roller is an important pest on rice. It has occurred more seriously in recent years and brought losses to rice production. Therefore, the adaptability of the insect to various environmental factors, the development of drug resistance, and the genetic variation of the population are studied. It is very important to reveal the law of population occurrence and development, so as to provide theoretical and methodological guidance for control management. Most of the above studies were carried out indoors, and the long-term and efficient indoor subculture of the rice leaf roller is an important guarantee for the smooth research. Regarding the indoor breeding of the rice leaf roller, there are existing methods of using rice, corn seedlings and artificial feed to raise the...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 刘向东廖怀建黄建荣方源松
Owner NANJING AGRICULTURAL UNIVERSITY
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