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Internal standard ion source device for quality correction

An ion source and internal standard technology, which is used in measuring devices, ion sources/guns, parts of particle separator tubes, etc., can solve problems such as difficulty in achieving the best effect, lengthening the distance of droplets with dots, and time-consuming and laborious operation. , to simplify the quality calibration process, reduce the time used, and save reagents

Inactive Publication Date: 2014-11-26
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, in order to ensure the accuracy of mass calibration, the signal conversion time should be as short as possible, and the number of signal alternations should be guaranteed to a certain level. These requirements are not only difficult to realize, but also time-consuming and labor-intensive to operate, and it is difficult to achieve the best effect. it is good
Nicolas L.Young et al. designed a steady-state asymmetric double nanospray calibration ion source. This device avoids mechanical conversion and voltage conversion, but due to the lengthening of the distance from the generation of the charged droplet to the mass spectrometer, the reagent's The dosage is still very large, and it is easy to cause a decrease in sensitivity

Method used

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  • Internal standard ion source device for quality correction
  • Internal standard ion source device for quality correction
  • Internal standard ion source device for quality correction

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1. Internal standard ion source device for mass spectrometry calibration

[0026] The internal standard ion source device used for mass spectrometry mass correction provided by this example includes two sample injection nanonozzles 1 and 2, a metal cylinder 3, a three-dimensional adjustable workbench 4, a glass support column 5 and a metal plate 7; the metal cylinder 3 The material of metal plate 7 is copper, and its length is 2cm, and shape is ellipsoid (just can put into two nanonozzles 1 and 2); The material of metal plate 7 is also copper, and it is connected with the outer wall of metal tube 3, and is Integral molding; the material of the sample injection nozzle 1 and 2 is glass, the inner diameter is 0.5 mm, and the length is 3 cm, and it is set in the cavity of the metal cylinder 3 and protrudes from its two ends; the metal plate 7 passes through the glass support column 5 is connected with the three-dimensional adjustable workbench 4, to carry out the re...

Embodiment 2

[0028] Application of the internal standard ion source device of embodiment 2, embodiment 1

[0029] The mass spectrum of the standard reserpine (purchased from Sigma) sample was measured and calibrated with the internal standard ion source device provided in Example 1.

[0030] (1) Simultaneous acquisition of sample and internal standard signal (The mass spectrometer used in this experiment is Thermo LTQ XL, which is only used to test the calibration signal and cannot be used for accurate mass calibration.)

[0031] With PEG-600 as the internal standard, reserpine and PEG were made into solvents with methanol / water (1:1) at a concentration of 10 -4 mol / L and 10 -4g / L solution, and then take an appropriate amount (about 8 μL) of the prepared solution and put them into the two pre-drawn sample injection nozzles 1 and 2, and put the two sample injection nozzles 1 and 2 side by side. After the metal cylinder 3 is placed in the metal cylinder 3, the metal cylinder 3 is fixed on ...

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Abstract

The invention discloses an internal standard ion source device for quality correction. The device comprises two sample injection nano spray pipes, a metal cylinder, a metal plate, a three-dimensional adjustable working table and a wire, wherein the sample injection nano spray pipes are arranged in the cavity of the metal cylinder and extend out of the two ends of the metal cylinder; the outer wall of the metal cylinder is connected with the metal plate; the metal plate is connected with the three-dimensional adjustable working table through an insulating supporting column; and the wire is connected with the metal plate. Since an alternating current induced ionization method is adopted, ions of a sample to be detected and ions of a reference can be simultaneously produced and detected, and mutual interference and inhibition between the two ions are effectively avoided; and since signals of the sample and the reference can be simultaneously obtained, and conversion and detection of the signals are not required, a quality calibration process can be simplified, the time for calibration is shortened, and a reagent is also saved.

Description

technical field [0001] The invention relates to an internal standard ion source device for mass calibration, in particular to an ion source device capable of simultaneously generating ion information of samples and reference objects. Background technique [0002] Accurate mass determination plays an important role in identifying unknown compounds. As the accuracy increases, the number of possible compositions of unknowns retrieved from the database will be greatly reduced, which will help to determine the composition and structure of unknown compounds more quickly and effectively. For example, when identifying a certain peptide, when the accuracy reaches ±1ppm, 99% of possible substances with the same apparent mass but different elements and amino acid compositions can be excluded, which makes the identification of unknowns more reliable. [0003] In accurate mass determination, high-resolution mass spectrometry is indispensable, but this alone is far from meeting the requi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01J49/16G01N27/64
Inventor 聂宗秀李亚峰张宁王佳宁
Owner INST OF CHEM CHINESE ACAD OF SCI