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Detection method for six-component costustoot preparation

A detection method and a preparation method technology are applied in the detection of pharmaceutical composition preparations and the detection of Liuweimuxiang preparations, and can solve the problems of high Rf value of pomegranate seeds, negative interference, poor repeatability and specificity, etc.

Active Publication Date: 2014-05-21
TIBET QIZHENG TIBETAN MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] It can be seen from the above that the TLC identification of Akiba, pomegranate seeds, and longan in the original ministerial standard was carried out using a unified extraction method and developing agent conditions. There is negative interference, the Rf value of pomegranate seeds is high, and the test solution of Piper longum does not have any spots. The repeatability and specificity of the standard are poor, and the controllability of the quality standard detection method for Liuwei Muxiang pills cannot be realized.

Method used

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  • Detection method for six-component costustoot preparation
  • Detection method for six-component costustoot preparation
  • Detection method for six-component costustoot preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0065] Experimental Example 1: TLC Identification of Woody Fragrance, Pomegranate Seed and Piper Longum

[0066] 1.1 Identify woody fragrance, pomegranate seed and longan longan according to the identification method issued by the ministry

[0067] Preparation of the test solution: take 2 g of the Liuwei Muxiang preparation, add 20 ml of ethyl acetate, reflux in a water bath at 60 to 80 ° C for 30 minutes, filter, and concentrate the filtrate to about 2 ml as the test solution;

[0068] Preparation of the reference medicinal material solution: take 0.5 g of each of the reference medicinal materials of cassava and pomegranate, and prepare the reference medicinal material solution in the same way;

[0069] Preparation of reference substance solution: take an appropriate amount of piperine reference substance, accurately weighed, add methanol to make a solution containing 1 mg of piperine per 1 ml, as the reference substance solution;

[0070] Preparation of woody negative sam...

experiment example 2

[0104] Experimental example 2: Determination of the content of corylidene and dehydrocorylides contained in coriander

[0105] (1) Instrument

[0106] Erlenmeyer flask, volumetric flask, pipette, syringe, beaker, funnel, filter paper, filter head, electronic balance (Mettler Toledo, model: XS205), high performance liquid chromatography (Agilent, USA, model: Aglient 1100 ).

[0107] (2) Reference substances: corylidene (batch number: 111524-201006) and dehydrocorylidene (batch number: 111525-200907), both purchased from China Institute for the Identification of Pharmaceutical and Biological Products.

[0108] (3) Reagents: methanol, acetonitrile, ethanol, water.

[0109] (4) Inspection method:

[0110] The choice of extraction solvent: use methanol and ethanol respectively;

[0111] Selection of extraction time: use ultrasound for 20 minutes, 30 minutes, and 45 minutes to test;

[0112] Choice of mobile phase: Acetonitrile-water (65:35), acetonitrile-water (60:40), metha...

experiment example 3

[0119] Experimental example 3: Determination of the content of coylin and dehydrocorylides contained in woody fragrance - methodology study

[0120] (1) Instruments and reagents

[0121] Agilent 1100 high performance liquid chromatograph (Agilent Corporation of the United States): G1314A UV-visible detector, G1314A binary pump, Agilent1100 chromatographic workstation;

[0122] SK8200HP ultrasonic cleaner (power 250W, frequency 40kHz) (Shanghai Kedao Ultrasonic Instrument Co., Ltd.);

[0123] Acetonitrile (chromatographically pure), methanol (analytical pure);

[0124]Cosmolide (batch number: 111524-201006) and dehydrocosmolide (lot number: 111525-200907) were purchased from China National Institute of Pharmaceutical and Biological Products;

[0125] Liuwei Muxiang preparation: provided by Tibet Qizheng Tibetan Medicine Co., Ltd.

[0126] (2) Chromatographic conditions

[0127] Octadecyl bonded silica gel as filler;

[0128] Chromatographic column: Kromasil E56526-C18 ...

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Abstract

The invention provides a detection method for a six-component costustoot preparation. The six-component costustoot preparation comprises six medicinal components, including costustoot, Veronica eriogyne H.Winkl, Phyllanthus emblica, cardamom, pomegranate seed and Fructus piperis longi. According to the method, costustoot, Veronica eriogyne H.Winkl, Phyllanthus emblica, cardamom, pomegranate seed and Fructus piperis longi in the six-component costustoot preparation are detected by using a thin layer chromatography method. The detection method has the advantages of good repeatability and stability, convenience in operation, high precision, high specificity, clear spot developing and high degree of separation. Through building of a reliable quality detection method with high specificity, the quality of the six-component costustoot preparation can be controlled effectively so that the quality of the six-component costustoot preparation become stable, safe and controllable.

Description

technical field [0001] The invention relates to a detection method of a pharmaceutical composition preparation, in particular to a detection method of a six-flavored woody fragrance preparation, which belongs to the technical field of detection of traditional Chinese medicines. Background technique [0002] Liuwei Muxiang Pill is a Tibetan medicine, which is recorded in the Tibetan medicine sub-volume of "Drug Standards of the Ministry of Health", and the standard number is WS3-BC-0283-95. The contents of this standard record mainly include: [0003] Prescription: Muxiang 200g, Pashaga 360g, Emlical emblica 500g, Cardamom 80g, Pomegranate 400g, Piper longum 100g. [0004] Preparation method: Grind the above six flavors into fine powder, sieve, mix well, pill with water, and dry to obtain. [0005] Properties: This product is a brown water pill; it has a specific woody aroma and tastes sour and bitter. [0006] Identification: Take 2g of this product, add 20ml of ethyl ace...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/02
Inventor 张国霞续艳丽陈丽娟
Owner TIBET QIZHENG TIBETAN MEDICINE