Transgene cotton embryogenic callus differential culture medium

A technology of differentiation medium and embryogenic callus, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problem of low rate of differentiation into seedlings of transgenic cotton embryogenic callus, and achieve improved vitrification phenomenon, not easy to contaminate, and the effect of improving seedling rate

Inactive Publication Date: 2012-08-29
COTTON RES INST SHANXI ACAD OF AGRI SCI
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a transgenic cotton embryogenic callus differentiation medium in order to solve the problem of low seedling rate of embryogenic callus differentiation in existing transgenic cotton

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Transgene cotton embryogenic callus differential culture medium
  • Transgene cotton embryogenic callus differential culture medium
  • Transgene cotton embryogenic callus differential culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] A kind of transgenic cotton embryogenic callus differentiation medium, its preparation method is: add MS macroelement, trace element, glucose, adjust pH value to 6.8, add curing agent Phytagel, the concentration of described curing agent Phytagel is 4.0 g / L, 4.05 g / L, 4.10 g / L, 4.15 g / L, 4.20 g / L, 4.25 g / L, 4.30 g / L, 4.35 g / L, 4.40 g / L, 4.45 g / L, 4.5 g / L L.

[0017] 1. Materials and methods

[0018] 1.1 Test material

[0019] Transgenic cotton embryogenic callus was selected as the experimental material.

[0020] 1.2 Production of transgenic cotton embryogenic callus differentiation medium

[0021] 1.2.1 Experiment 1

[0022] MS macroelements and trace elements (MS macroelements and trace elements are both routine dosages), glucose 30g / L;

[0023] 10% KOH to adjust the pH to 6.8;

[0024] The amount of curing agent Phytagel was set up for 14 groups of comparative tests: 2.0g / L, 2.5 g / L, 2.8 g / L, 3.0 g / L, 3.3 g / L, 3.5 g / L, 3.8 g / L, 4.0 g / L L, 4.2 g / L, 4.5 g / L, 4....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of transgene cotton tissue culture, in particular to a transgene cotton embryogenic callus differential culture medium, which solves the problem of lower seedling rate of the existing transgene cotton embryogenic callus differentiation. A manufacture method of the culture medium comprises the following steps that: mass spectrum (MS) major elements, trace elements and glucose are added, the pH value is regulated to 6.8, curing agent Phytagel is added, and the concentration of the curing agent Phytagel is 4.0 to 4.5g / L. The transgene cotton embryogenic callus differential culture medium provided by the invention has the advantages that the concentration of the curing agent Phytagel is obviously improved, the pollution phenomenon cannot be easily generated, the growth and the development of the embryogenic callus are favorably realized, and the vitrification phenomenon of young differentiated seedlings can be effectively improved, so the embryogenic callus differential seedling emergency percentage is obviously improved.

Description

technical field [0001] The invention relates to the field of transgenic cotton tissue culture, in particular to a transgenic cotton embryogenic callus differentiation medium. Background technique [0002] The purpose of cotton transgenic is to transfer the exogenous target gene into conventional cotton, and use tissue culture biotechnology to cultivate new cotton germplasm resources with the purpose of disease resistance, insect resistance, strong stress tolerance and good quality. The conventional tissue culture The method is as follows: firstly, the hypocotyls of the recipient cotton are infected with Agrobacterium carrying the exogenous target gene, and after co-cultivation for 48 hours, after callus induction, the callus is induced into an embryoid body (embryogenic callus). Callus differentiation to generate regenerated strains (embryogenic callus differentiated into seedlings), regenerated strains transplanted and other steps. Among them, in the whole culture proc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马燕斌吴霞上官小霞王霞李燕娥张相斌段晓康韩青龙马冬菊崔婷原辉
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap