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Ophiobolin sesterterpene compound and preparation and application thereof

A technology of pseudochicin and sesquiterpene, applied in biochemical equipment and methods, microorganisms, organic chemistry, etc., can solve the problems of serious residue, soil and water pollution, refractory degradation, etc., and achieve obvious antibacterial activity Effect

Active Publication Date: 2012-09-12
北海南方海洋科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method allows for more precise analysis on pseudomonal bacillus strains by analyzing their ability to produce certain types of chemical substances called quaternary alkaline phosphatase (QAP). These methods have been shown effective against various microorganisms such as Bacillus subtilis or Staphylococcus sp., indicating they can be used effectively with other organism-related diseases like malaria.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing novel chemical compounds or agents effective against various types of germs such as Bacilluses, staphylcystis, pseudomonads, Enterobacters, Salmonella spp., Listeria monocytosis, Clostridum perfringera, Yersinioannoumaras, etc.

Method used

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  • Ophiobolin sesterterpene compound and preparation and application thereof
  • Ophiobolin sesterterpene compound and preparation and application thereof
  • Ophiobolin sesterterpene compound and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The sesquiterpene compound of the pseudochicin class derived from seaweed endophytic fungi is represented by the formula (I).

[0021]

Embodiment 2

[0023] The preparation method of the pseudochicin class sesquiterpene compound shown in formula (I):

[0024] Take the fungus Aspergillus ustus cf-42 that grew well on the plate, cut it into small pieces and inoculate it in the PDB liquid medium, put 300mL medium in each 1L Erlenmeyer flask, 50 bottles in total, ferment at room temperature for 35 days, add One-half volume of ethyl acetate in the fermented liquid kills the fungus, is filtered, and the mycelium and the fermented liquid are collected respectively. The PDB liquid medium consists of 200 milliliters of potato juice per liter, 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract, 500 milliliters of aged sea water, and 300 milliliters of distilled water. Among them, the fungus Aspergillus ustus cf-42 strain was preserved in the China Center for Type Culture Collection CCTCC on November 25, 2011, the preservation number is CCTCC M2011420, the classification name is Aspergillus ustus cf-42, the preservation...

Embodiment 3

[0032] The difference from Example 2 is that

[0033] Take the fungus Aspergillus ustus (Aspergillus ustus) cf-42 strain that grew well on the plate, cut it into small pieces and inoculate it in PDB liquid medium, put 300mL medium in each 1L Erlenmeyer flask, 50 bottles in total, and ferment statically at room temperature for 35 days , adding 1 / 2 volume of ethyl acetate to the fermentation broth to kill the fungi, filtering, and collecting the mycelia and fermentation broth respectively. The PDB liquid medium consists of 200 milliliters of potato juice per liter, 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract, 500 milliliters of aged sea water, and 300 milliliters of distilled water.

[0034] Collect about 15L of fermented liquid, extract three times with ethyl acetate, concentrate under reduced pressure; extract three times with acetone after the mycelia is pulverized, then extract with ethyl acetate, concentrate under reduced pressure; Thin-layer chromatogra...

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Abstract

The invention relates to fields of pesticides and bacterial inhibitors, which particularly relates to an ophiobolin di-sesquiterpene compound derived from seaweed entophytic fungi, and preparation and application thereof. The structural formula is shown in a formula (I). The preparation method comprise: inoculating Aspergillus ustus cf-42 in a fungus liquid medium to ferment and culture. After purification, the fermentation product is the ophiobolin sesterterpene compound as shown in the formula (I). The ophiobolin di-sesquiterpene compound obtained by the invention can cause a higher death rate of artemia salina sinnaeu which is 78.4% when the compound concentration is 100 microgram/ml. An insecticidal activity experiment suggests that the median lethal concentration is 48.1 microgram/ml. At the meantime, this compound also possesses substantial bacteriostatic activity.

Description

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Claims

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Application Information

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Owner 北海南方海洋科技开发有限公司
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