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Method for measuring cystine through surface-enhanced raman spectroscopy

A surface-enhanced Raman and spectroscopic measurement technology, applied in the field of analytical chemistry, achieves the effects of good selectivity, good stability, and easy operation

Inactive Publication Date: 2014-01-08
GUANGXI NORMAL UNIV
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Problems solved by technology

The method of measuring cystine based on nano-silver-2-mercaptopyridine surface-enhanced Raman spectroscopy has not been reported yet

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  • Method for measuring cystine through surface-enhanced raman spectroscopy

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Embodiment

[0019] A method for measuring cystine with surface-enhanced Raman spectroscopy, comprising the steps of:

[0020] (1) Preparation of cystine standard test system: In a 5 mL graduated test tube, add 750 μL 19.9 mg / L nano-silver solution, 100 μL 0.2 mol / L pH 6.6 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution in sequence , 40 μL 1.0×10 -5 mol / L 2-mercaptopyridine solution, 10, 20, 30, 50, 60 μL 2.0×10 -5 mol / L cystine, 70 μL 2.0 mol / L sodium chloride solution, shake well, dilute to 2.0 mL with double-distilled water, and place in an ultrasonic instrument for 15 minutes;

[0021] (2) Prepare the blank control system without adding cystine according to the method of step (1);

[0022] (3) Take the solutions of the above systems and place them in quartz cuvettes, set the instrument parameters on the DXR smart Raman spectrometer, scan to obtain the surface-enhanced Raman scattering spectrum of the system, and measure 1002 cm -1 The surface-enhanced Raman...

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Abstract

The invention discloses a method for measuring cystine through surface-enhanced raman spectroscopy. The method includes the following steps: 1 preparing analysis solution of cystine with known concentration, and measuring that the strength value of a surface-enhanced raman scattering peak at the position of 1002cm-1 is I; 2 preparing a blank contrast system free of cystine, and measuring that the strength value of a surface-enhanced raman scattering peak is I0; 3 calculating that delta I=I0-I; 4 making a working curve for cystine concentration C by aid of the delta I; 5 preparing analysis solution of a sample to be detected, measuring that the strength value of a surface-enhanced raman scattering peak of the analysis solution is I sample, and calculating that delta I sample =I0-I sample; and 6 calculating concentration of cystine in the sample to be detected according to the working curve. Compared with the existing method, the measuring method is simple and convenient to operate, high in sensitivity and good in selectivity, and reagents can be obtained easily.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for measuring cystine by surface-enhanced Raman spectroscopy. Background technique [0002] Cystine is an amino acid drug, which can promote the oxidation and reduction of body cells, increase white blood cells and prevent the development of pathogenic bacteria. It is mainly used for the treatment of secondary alopecia and chronic hepatitis. Therefore, the quality control of drugs is of great significance. At present, the methods for detecting cystine mainly include single-scan polarography, atomic absorption spectrometry, titration, colorimetry, fluorescence spectroscopy, spectrophotometry, chemiluminescence, stripping voltammetry, resonance scattering spectroscopy, etc. . Among these methods for measuring cystine, some are complicated to operate, and some are not sensitive enough, so it is necessary to seek a sensitive method. Surface-enhanced Raman spectroscopy ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 蒋治良韦燕燕梁爱惠
Owner GUANGXI NORMAL UNIV