Biomedical chip used for blood coagulation tests, its manufacturing method and application

Abstract

The invention relates to a biomedical chip used for blood coagulation tests, its manufacturing method and application. The biomedical chip used for blood coagulation tests contains a substrate layer, an intermediate layer and a top cover layer in superposed connection, and the substrate layer, the intermediate layer and the top cover layer cooperate to define a microchannel, and a first injection port, an outlet and a second injection port that are respectively connected to the microchannel. An outward expanded mixing zone of the microchannel has connecting portion communicated with the second injection port, and a capillary portion disposed between the substrate layer and the top cover layer, set around and communicated with the connecting portion. With the biomedical chip composed of the substrate layer and the top cover layer prepared from a hydrophilic material, the intrinsic capillary force of the microchannel can drive the flow and mixing of blood and a reagent, and a hydrophilic capillary force can be maintained permanently.

Description

technical field [0001] The invention relates to a biomedical chip, in particular to a biomedical chip that can be used to mix and deliver two reagents and can be used for blood coagulation testing. Background technique [0002] The traditional blood coagulation test must be done in a laboratory. The test sample needs to be the plasma after blood centrifugation. It takes time to obtain the test sample, and the equipment used is either too expensive or too bulky, and it needs to be operated by professionals. , does not meet the needs of the general public for real-time use, and it is also quite troublesome for medical staff who urgently need to know the experimental results at the first time. Although there are several small machines related to blood coagulation, their service targets are usually limited to patients taking anticoagulant drugs, and are not suitable for the general public. [0003] In recent years, the development technology of biomedical detection chip has bee...

AI Technical Summary

Problems solved by technology

However, take the method of improving the hydrophilicity of PDMS by oxygen plasma and heat treatment as an example. When only oxygen plasma treatment is performed, the high hydrophilicity will disappear after tens of minutes. If the oxygen plasma is modified Further chemical immersion treatment of PDMS can maintain the hydrophilicity for several days, but the problem of water repellency return still exists

[0005] In addition to polymer materials such as PDMS and SU8, glass is also a commonly used material for fluid wafers, but the fabrication and bonding of traditional glass wafers have disadvantages such as complicated process steps and high thermal bonding temperature.

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