Seed breeding method of moso bamboo by physical and chemical composite mutagenesis

Abstract

The invention discloses a seed breeding method of moso bamboo by physical and chemical composite mutagenesis. The seed breeding method comprises the following five steps of: (1) collection of the moso bamboo seeds, wherein the purity of the seeds is more than or equal to 90%, the thousand seed weight is more than or equal to 28g, the moisture content is 10%-14%, and after being sealed, the seeds are stored for later use at the temperature of 4 DEG C; (2) irradiation ray selection of caesium Gamma rays with the atomic weight being 137 as an irradiation source, and selection of two types of mutagen treating liquid; (3) preparation of the two types of mutagen treating liquid; (4) physical and chemical composite mutagenesis treatment, wherein the radiation dose rate is 1Gy/min, the radiation dose is 30Gy, the seeds are soaked for tens of hours with warm water, after water-absorbing paper absorbs the surface water of the seeds completely, the seeds are soaked and mutagenized for eight hours by using a sodium azide solution (NaN3) or ethylmethane sulfonate (EMS) solution with the concentration of 0.04mmol/L, and after being taken, the seeds are cleaned repeatedly for 2 hours by using clean water to obtain the moso bamboo seeds treated by composite mutagenesis; and (5) germination test of the seeds, verification of mutagenic effect and determination of mutagenesis dose. The seed breeding method disclosed by the method has the advantages that the mutants in mutagenesis are more in quantity, the character mutation is diversified, and the selection range of excellent characters needed in seed breeding is large.

Description

technical field [0001] The invention relates to a physical and chemical compound mutation breeding method for plant seeds, specifically carrying out gamma ray irradiation of cesium and sodium azide (NaN 3 ) or ethyl methanesulfonate (EMS) mutagen treatment liquid immersion compound mutation breeding method. Background technique [0002] Moso bamboo (Phyllostachys heterocycla cv. Pubescens) is an excellent bamboo species with extremely fast growth, early maturity, high yield and long profit period. Moso bamboo seldom blooms and sets seeds, and once it blooms, it will decline immediately, so cross-breeding cannot be carried out, and the genetic improvement of moso bamboo is relatively neglected. "Anhui Agricultural Science" 37 volumes 17 published the "Preliminary Report on the Research of Moso Bamboo Radiation Mutation Breeding Seedlings" by Cai Xinling and other scholars, introducing the use of 60 Co-γ is the radiation source, the dose rate is 1.86Gy / min, and the optimal i...

AI Technical Summary

Problems solved by technology

The mechanisms of physical mutagenesis and chemical mutagenesis are different, and the types of mutations induced are different. Combining the two mutagenesis methods, that is, compound mutagenesis, is used to treat bamboo

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