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Compound papain composition and application thereof
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A papain and composition technology, applied in the field of composite papain composition, can solve the problems of low enzyme activity and limited application fields, and achieve the effects of function expansion, activity improvement and preparation cost reduction
Inactive Publication Date: 2013-12-11
牛安霖
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Problems solved by technology
Now some enterprises have begun to produce papain, but most products have relatively low enzyme activity and limited application fields.
Method used
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preparation example Construction
[0033] (1). Preparation of Folin's reagent: Add 100g of sodium tungstate (Na 2 WO 4 -2H 2 O), 25g sodium molybdate (Na 2 MoO 4 -2H 2 (0), 700mL water, 50mL 85% phosphoric acid, 100mL concentrated hydrochloric acid, boil and reflux in a small fire for 10h, remove the reflux device, and add 50g lithium sulfate (Li 2 SO 4 ), 50mL of water and a few drops of concentrated bromine water (99%), boiled slightly for 15 minutes to remove excess bromine, cooled, added water to make up to 1000mL, mixed well, filtered, and stored in a brown bottle;
[0034] (2). Use solution: mix one part of Folin reagent with two parts of water, and shake well;
[0035] (3).0.4mol / L sodium carbonate solution (Na 2 CO 3 );
[0036] (4).0.4mol / L trichloroacetic acid;
[0037] (5).0.5mol / L sodium hydroxide solution (NaOH);
[0038] (6). 1 mol / L hydrochloric acid solution (HCl) and 0.1 mol / L hydrochloric acid solution (HCl);
[0075] Add xylo-oligosaccharide mass (g): papain activity (u) is 0.002:1000, according to the experimental method described in the above-mentioned formal test, the absorbance is tested to be 0.0428, which is obtained by using the above formula for calculating the increase rate. This example provides The papain activity (u) of papain was increased by 5.42% relative to the papain activity (u) of the comparison.
Embodiment 2
[0077] Add xylo-oligosaccharide mass (g): papain activity (u) is 0.01:1000, according to the experimental method of the above-mentioned sample test, its absorbance is 0.0438, which is obtained by using the above formula for calculating the increase rate. The papaya provided in this example The protease activity (u) was increased by 7.88% compared to the papain activity (u) of the comparative example.
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Abstract
Disclosed is a compound papain composition, which comprises xylo-oligosaccharide and papain, wherein a ratio of the mass (g) of the xylo-oligosaccharide and the activity (u) of the papain is equal or greater than 0.002:1000. The compound papain composition of the invention is prepared by adding the xylo-oligosaccharide into the traditional papain, thereby increasing the activity of the papain. Further disclosed in the invention is the application of the compound papain composition in medicine, health protection industry, food industry, cosmetic industry, daily-use chemicals industry, feed industry, and leather industry. According to the invention, good effects can be obtained at a low cost.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a compound papain composition and application thereof. Background technique [0002] Papain (Papain) is referred to as papain, also known as papaya enzyme. It is a pure natural biological enzyme product extracted from the milk of immature papaya (Carica papaya) fruit and refined by modern bioengineering technology. It is a sulfhydryl group (-SH)-containing endopeptidase, which has protease and esterase activity, has a wide range of specificity, and has strong hydrolysis ability for animal and plant proteins, polypeptides, esters, amides, etc. At the same time, it also has a synthetic function, which can synthesize protein hydrolyzate into proteinoids. It is used in a wide range of industries, including: pharmaceutical industry, food industry, beauty cosmetics industry, feed industry, leather industry, textile industry, etc. In the pharmaceutical industry, drugs containing papain c...
Claims
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