Biological recovery method of microalgae

Abstract

The invention relates to a recovery method of microalgae, in particular to a biological recovery method of microalgae. The method includes: purifying bivalve shellfish temporarily cultured in a pond; and detecting purification effects of the purified shellfish by randomly drawing thirty shellfish, cleanly rinsing the thirty shellfish with distilled water, opening shells, taking soft tissues prior to triturating the soft tissues with a homogenizer, and detecting microorganisms and volatile basic nitrogen. The biological recovery method of the microalgae is low in cost, high in efficiency, low in damage to the microalgae and wide in application range of microalgal concentration.

Description

technical field

[0001] The invention relates to a method for harvesting microalgae, in particular to a method for biologically harvesting microalgae. Background technique

[0002] Microalgae is a type of tiny algae composed of single cells or several cells, with a diameter of 3-30 μm, mostly eukaryotes. Microalgae grow fast, have high output per unit area, and are rich in nutrients, such as protein, vitamins and unsaturated fatty acids. It is widely used in the fields of biological bait, health food and pharmaceuticals. In recent years, the application of microalgae as biodiesel has attracted much attention.

[0003] At present, the cost of harvesting microalgae is extremely high, accounting for 20% to 30% of the cost of cultivation (Molina G E, Belarbi E H, Acien Fernandez F G, et al. Recovery of Microalgal Biomass and Metabolites: Process Options and Economics. Biotechnol. Adv. , 2003, (20): 491-515). Reducing harvesting costs is one of the keys to microalgae productio...

Examples

Embodiment Construction

[0028] The present invention comprises the following steps:

[0029] 1) Temporarily keep bivalve molluscs in a pool for shellfish purification;

[0030] The purification can firstly clean the shell surface of bivalve molluscs, remove dead individuals and shell damaged individuals, and then discharge the shellfish into a leaky plastic tray, and the size of the leakable plastic tray can be 60mm×40mm×15mm , one plate per 10kg, put 100 plates in a 6m×4m×1.2m purified water pool; irradiate the purified seawater that meets the seawater quality standard for fishery with ultraviolet rays, and the dose reaches 300J / m 2 , and then injected into the purification pool to make the water depth reach 50cm, keep the water temperature at 20-25°C, use an aerator to keep the dissolved oxygen above 4mg / L, change the water every 8h, and the total purification time is 48h. During the purification process, dead individuals are found to be eliminated immediately, and dead individuals and shell damag...

technical field

[0001] The invention relates to a method for harvesting microalgae, in particular to a method for biologically harvesting microalgae. Background technique

[0002] Microalgae is a type of tiny algae composed of single cells or several cells, with a diameter of 3-30 μm, mostly eukaryotes. Microalgae grow fast, have high output per unit area, and are rich in nutrients, such as protein, vitamins and unsaturated fatty acids. It is widely used in the fields of biological bait, health food and pharmaceuticals. In recent years, the application of microalgae as biodiesel has attracted much attention.

[0003] At present, the cost of harvesting microalgae is extremely high, accounting for 20% to 30% of the cost of cultivation (Molina G E, Belarbi E H, Acien Fernandez F G, et al. Recovery of Microalgal Biomass and Metabolites: Process Options and Economics. Biotechnol. Adv. , 2003, (20): 491-515). Reducing harvesting costs is one of the keys to microalgae productio...

Examples

Embodiment Construction

[0028] The present invention comprises the following steps:

[0029] 1) Temporarily keep bivalve molluscs in a pool for shellfish purification;

[0030] The purification can firstly clean the shell surface of bivalve molluscs, remove dead individuals and shell damaged individuals, and then discharge the shellfish into a leaky plastic tray, and the size of the leakable plastic tray can be 60mm×40mm×15mm , one plate per 10kg, put 100 plates in a 6m×4m×1.2m purified water pool; irradiate the purified seawater that meets the seawater quality standard for fishery with ultraviolet rays, and the dose reaches 300J / m 2 , and then injected into the purification pool to make the water depth reach 50cm, keep the water temperature at 20-25°C, use an aerator to keep the dissolved oxygen above 4mg / L, change the water every 8h, and the total purification time is 48h. During the purification process, dead individuals are found to be eliminated immediately, and dead individuals and shell damag...