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Method for catalytic synthesis of fructose lauric acid monoester by using immobilized phospholipase A1

A technology for immobilizing phospholipase and lauric acid monoester, which is applied in the field of fructose lauric acid monoester, can solve problems that have not been seen, and achieve the effects of low toxicity, low boiling point, and good industrial application prospects

Inactive Publication Date: 2014-07-02
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the preparation of fructose laurate monoester from fructose and lauric acid catalyzed by immobilized phospholipase A1

Method used

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  • Method for catalytic synthesis of fructose lauric acid monoester by using immobilized phospholipase A1
  • Method for catalytic synthesis of fructose lauric acid monoester by using immobilized phospholipase A1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] a. Weigh 5 g of activated DA-201 macroporous adsorption resin, mix 10 g of phosphate buffer solution, and 1 g of free phospholipase A1, stir and adsorb at 25°C for 2 hours, and then filter to obtain macroporous adsorption resin immobilized phospholipase , its enzyme activity is 1850 U / g. Vacuum dried, ready for use;

[0026] b. Place the 4? molecular sieve in an oven at 105°C for 10 hours, then place it in a desiccator and cool it to room temperature for later use; after that, add the activated 4? in tert-butanol, sealed and placed for 72h, and filtered to remove molecular sieves to obtain a dehydrated organic solvent;

[0027] c. Enzymatic esterification: Mix 10g of lauric acid and 1.8g of fructose in the above-mentioned dehydrated organic solvent with a volume of 42ml, then add 2g of immobilized phospholipase A1 to start the reaction; after 2 hours of reaction, add 10g of activated 4? Generated water; shake or stir the reaction at 45°C for 48 hours, and the shakin...

Embodiment 2

[0029] a. Weigh 5 g of activated DA-201 macroporous adsorption resin, mix with 20 g of phosphate buffer, and 1 g of free phospholipase A1, stir and adsorb at 30°C for 4 hours, and then filter to obtain macroporous adsorption resin immobilized phospholipase. Its enzyme activity is 1462 U / g. Vacuum dried, ready for use;

[0030] b. Place the 4? molecular sieve in an oven at 100°C for 8 hours, then place it in a desiccator and cool it to room temperature for later use; after that, add the activated 4? in tert-amyl alcohol, sealed for 24 hours, filtered to remove molecular sieves, to obtain a dehydrated organic solvent;

[0031] c. Enzymatic esterification: Mix 10g of lauric acid and 2.3g of fructose in the above-mentioned dehydrated organic solvent with a volume of 100ml, then add 2g of immobilized phospholipase A1 to start the reaction; add 10g of activated 4? Generated water; shake or stir the reaction at 40°C for 48 hours, and the shaking speed is 160r / min; It can be know...

Embodiment 3

[0033] a. Weigh 5 g of activated DA-201 macroporous adsorption resin, mix with 20 g of phosphate buffer, and 1 g of free phospholipase A1, stir and adsorb at 35°C for 3 hours, then filter to obtain macroporous adsorption resin immobilized phospholipase, Its enzyme activity is 1344 U / g. Vacuum dried, ready for use;

[0034] b. Place the 3? molecular sieve in an oven at 95°C to activate for 5 hours, and then place it in a desiccator to cool to room temperature for later use; after that, add the activated 3? molecular sieve to the reaction medium in an amount of 50 g per liter of solvent in acetone, sealed for 72 hours, and filtered to remove molecular sieves to obtain a dehydrated organic solvent;

[0035] c. Enzymatic esterification: Mix 40.6g of lauric acid and 36.5g of fructose in the above-mentioned dehydrated organic solvent with a volume of 812ml, then add 8.12g of immobilized phospholipase A1 to start the reaction; after 1 hour of reaction, add 40.6g of activated 3? mo...

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Abstract

The invention discloses a method for preparing fructose laurate monoester catalyzed by immobilized phospholipase A1. The method is to synthesize fructose laurate monoester through enzymatic catalytic reaction of lauric acid and fructose. The preparation method of the present invention comprises the following steps: (1) immobilization of phospholipase A1; (2) preparation of a dehydrated organic solvent; (3) catalyzed synthesis of fructose laurate monoester by immobilized phospholipase A1. The price of the phospholipase A1 used in the invention is cheap, only 5%-10% of the price of the lipase, and the price of the immobilized resin DA-201 used for the enzyme is low. The immobilized phospholipase A1 has a good catalytic effect, the conversion rate of fructose laurate monoester is higher, the separation of the product is simple, and the immobilized enzyme can be reused, so the cost of the product prepared by this method is relatively low, and it is widely used in industry. It has a good application prospect.

Description

technical field [0001] The invention relates to an immobilized phospholipase A1 catalyzing the synthesis of a sugar ester surfactant-fructose laurate monoester, which belongs to the field of organic synthesis. Background technique [0002] Sugar esters are generally formed by linking long-chain fatty acids and sugars through ester bonds. Therefore, sugar ester molecules have non-polar long-chain fatty acids and polar polyhydroxy sugars at the same time, thus having the effect of surfactant. Sugar ester molecules do not have ionizing functional groups and are typical nonionic surfactants. As a typical nonionic green biosurfactant with excellent performance, it has important uses in industries such as food, cosmetics, medicine, detergent, and fiber. Fructose esters, as a class of nonionic surfactants, also have a wide range of applications. [0003] There are chemical and enzymatic methods for the synthesis of sugar esters. The chemical method is often carried out under hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02
Inventor 赵谋明赵强忠崔春付敏刘宁仇超颖
Owner SOUTH CHINA UNIV OF TECH