Microfluidic chip electrophoretic-electrochemical detecting device with adjustable pH after separation and use thereof

A microfluidic chip and detection device technology, applied in measurement devices, chemical instruments and methods, scientific instruments, etc., can solve problems such as damage to electrophoretic separation, high current, air bubbles, etc., achieve reliable drug analysis devices and methods, and improve stability. Effects of Sex and Sensitivity

Inactive Publication Date: 2012-11-21
GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when a large separation voltage is used under strong alkali conditions, a large current and Joule heat will be generated in the microchannel, which will easily lead to the generation of air bubbles, thereby destroying the electrophoretic separation.

Method used

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  • Microfluidic chip electrophoretic-electrochemical detecting device with adjustable pH after separation and use thereof
  • Microfluidic chip electrophoretic-electrochemical detecting device with adjustable pH after separation and use thereof
  • Microfluidic chip electrophoretic-electrochemical detecting device with adjustable pH after separation and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1. Microfluidic Chip Electrophoresis-Electrochemical Detection Device with Adjustable pH After Separation

[0032] like figure 1 As shown, the microfluidic chip electrophoresis-electrochemical detection device with adjustable pH after separation provided by the present invention includes a microfluidic chip body, and the microfluidic chip body includes a separation channel 8, and one end of the separation channel 8 is respectively It is connected with the sample pool 1, the buffer solution pool 2 and the sample waste pool 3, and forms a double T-shaped channel 7; the other end of the separation channel 8 is sequentially connected with the W-shaped channel 10, the electrode placement channel 12, and the buffer waste pool 6 are connected; the width and height of the W-shaped channel 10 are 50 μm, and the length is 2 mm; the electrode placement channel 12 is provided with a working electrode 11, and the working electrode 11 is a Pt plated with a Cu-Cr-Sn nano-alloy...

Embodiment 2

[0041] Example 2. Application of the PDMS microfluidic chip electrophoresis-electrochemical detection device provided by the present invention in the analysis of aminoglycoside antibiotics

[0042] like figure 1 Shown, when using the present invention, add high-concentration alkaline solution (0.1M NaOH), and the height of its liquid level is greater than other The height of the liquid level in the liquid storage area (sample pool 1, buffer pool 2, sample waste pool 3 and buffer waste pool 6) is about 1 times, and the static pressure difference of the solution is used to drive the alkaline solution pool 4 and 5 The strong alkaline solution and the acidic buffer solution from the separation channel merge into the end of the separation channel 8, and mix through the W-shaped channel 10, so that the pH value of the solution in the detection area 13 is greater than 12, so that the non-strong alkaline separation can be effectively realized. Under buffer solution conditions, microf...

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Abstract

The invention discloses a microfluidic chip electrophoretic-electrochemical detecting device with adjustable pH after separation and use of the device in aminoglycoside antibiotics analysis. The analysis device comprises a double-T shaped sampling channel, a separating channel, two auxiliary channels, a W-shaped channel and an electrode placement channel. Through adding the two auxiliary channels and the W-shaped channel at the tail end of the separating channel in a microfluidic chip, an alkaline solution is added through the auxiliary channels after being electrophoretically separated, and the solution from the separating channel and the auxiliary channels is mixed evenly through the W-shaped channel, a pH value of the solution in a working electrode determining region is improved, so that the solution meets a strong alkaline condition needed by the detection of carbohydrates, simultaneously, electrophoretic separation under an acidic separation buffer solution is not influenced. By the device disclosed by the invention, a transition metal nano material is decorated on the surface of the electrode by using an electrodeposition method and by means of the relatively large specific surface area and the special catalytic property of the metal nano material, so that the working electrode with high performance is obtained, and aminoglycoside antibiotics are determined through the amperometry.

Description

technical field [0001] The invention relates to a microfluidic chip electrophoresis-electrochemical detection device whose pH can be adjusted after separation and its application in the analysis of aminoglycoside antibiotics, and belongs to the technical field of drug analysis and detection of a micro-total analysis system. Background technique [0002] Aminoglycoside antibiotics are a class of glycoside antibiotics composed of two or more aminosugars and aminocyclic alcohols connected through oxygen bridges, such as spectinomycin, streptomycin, amikacin, paromomycin, neomycin It is mainly used to fight against the infection of Gram-negative and positive bacteria. Clinically, aminoglycoside antibiotics are widely used in the treatment of various serious bacterial infections, but these drugs show strong side effects such as ototoxicity, nephrotoxicity, nerve and muscle blockage, and allergic reactions, which can seriously cause hearing and renal dysfunction. The damage, even...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447B01L3/00
Inventor 丁永胜索兴梅孟祥英白亮
Owner GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS
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