D-dimer detection reagent kit and preparation method thereof
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A detection kit and dimer technology, applied in the field of biomedicine, can solve problems such as being unsuitable for routine inspection, cumbersome operation, waste of resources, etc.
Inactive Publication Date: 2012-11-21
乐普(北京)诊断技术股份有限公司
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Problems solved by technology
These methods have the disadvantages of cumbersome operation, long time consumption, excessive waste of resources, and are not suitable for routine inspection.
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Embodiment 1
[0033] The kit of the present invention is exemplified as a double reagent, wherein:
[0034] Reagent R1:
[0035] Tris buffer 200mmol / L
[0036] Polyethylene glycol 8 mmol / L
[0037] Sodium azide 5 mmol / L
[0038] Sodium edetate 5mmol / L
[0039] Bovine serum albumin 5mmol / L;
[0040] Reagent R2:
[0041] Tris buffer 200mmol / L
[0042] Latex particles conjugated with D-dimer monoclonal antibody 5% (v / v)
[0043] Tween-20 5mmol / L
[0044] Sodium azide 3 mmol / L
[0045] Sodium edetate 5mmol / L
[0046] Bovine serum albumin 5mmol / L.
Embodiment 2
[0047] Embodiment 2: Kit usage method.
[0048] 1. Reagent preparation, the reagent is a liquid double reagent, ready to use after opening the bottle, of which:
[0049] Reagent R1:
[0050] Tris buffer 200mmol / L
[0051] Polyethylene glycol 8 mmol / L
[0052] Sodium azide 5 mmol / L
[0053] Sodium edetate 5mmol / L
[0054] Bovine serum albumin 5mmol / L;
[0055] Reagent R2:
[0056] Tris buffer 200mmol / L
[0057] Latex particles conjugated with D-dimer monoclonal antibody 5% (v / v)
[0064] (b) Detection wavelength: the main wavelength is 570nm;
[0065] (c) Reaction time: 8 minutes, wherein the incubation time is 5 minutes, the reaction time is 10 seconds, and the detection time is 3 minutes.
[0066] 3. Detection steps (all completed in t...
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Abstract
A D-dimer detection reagent kit, the kit body of the reagent kit comprises two reagents inside: reagent R1: buffer solution, polyethylene glycol, sodium azide, sodium ethylene diamine tetracetate, and bovine serum albumin; reagent R2: buffer solution, latex particles bound with D-dimer monoclonal antibody, Tween-20, sodium azide, sodium ethylene diamine tetracetate, and bovine serum albumin. Mixing a sample with the reagents in a specific volumetric ratio to conduct a series of reactions, placing the reactant under a semi / full automatic biochemical analyzer, and detecting the change speed of light absorbance at the position of 570nm dominant wavelength, thus the concentration of D-dimer can be figured out. The reagent kit has the advantages of accuracy, stability and convenience.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a detection kit for detecting human D dimer. Background technique [0002] The fibrinolytic system is the most important anticoagulant system in the human body. When a fibrin clot is formed, the process of fibrinolysis begins, and dissolves the fibrin clot to form various soluble fragments, D-dimer is one of the most important soluble fragments. [0003] The fibrinolytic system of the human body plays an important role in maintaining the normal permeability of blood vessel walls, maintaining blood flow and tissue repair. Increased levels of D-dimer plasma indicate the presence of secondary fibrinolytic processes. Thrombolytic therapy refers to the use of drugs to activate the fibrinolytic system. Generally, a plasminogen activator such as urine enzyme, streptokinase or tissue-type plasminogen activator is put in to generate a large amount of plasminogen, thereby accelerati...
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