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D-dimer detection reagent kit and preparation method thereof

A detection kit and dimer technology, applied in the field of biomedicine, can solve problems such as being unsuitable for routine inspection, cumbersome operation, waste of resources, etc.

Inactive Publication Date: 2012-11-21
乐普(北京)诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of cumbersome operation, long time consumption, excessive waste of resources, and are not suitable for routine inspection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The kit of the present invention is exemplified as a double reagent, wherein:

[0034] Reagent R1:

[0035] Tris buffer 200mmol / L

[0036] Polyethylene glycol 8 mmol / L

[0037] Sodium azide 5 mmol / L

[0038] Sodium edetate 5mmol / L

[0039] Bovine serum albumin 5mmol / L;

[0040] Reagent R2:

[0041] Tris buffer 200mmol / L

[0042] Latex particles conjugated with D-dimer monoclonal antibody 5% (v / v)

[0043] Tween-20 5mmol / L

[0044] Sodium azide 3 mmol / L

[0045] Sodium edetate 5mmol / L

[0046] Bovine serum albumin 5mmol / L.

Embodiment 2

[0047] Embodiment 2: Kit usage method.

[0048] 1. Reagent preparation, the reagent is a liquid double reagent, ready to use after opening the bottle, of which:

[0049] Reagent R1:

[0050] Tris buffer 200mmol / L

[0051] Polyethylene glycol 8 mmol / L

[0052] Sodium azide 5 mmol / L

[0053] Sodium edetate 5mmol / L

[0054] Bovine serum albumin 5mmol / L;

[0055] Reagent R2:

[0056] Tris buffer 200mmol / L

[0057] Latex particles conjugated with D-dimer monoclonal antibody 5% (v / v)

[0058] Tween-20 5mmol / L

[0059] Sodium azide 3 mmol / L

[0060] Sodium edetate 5mmol / L

[0061] Bovine serum albumin 5mmol / L.

[0062] 2. Automatic biochemical analyzer parameter setting

[0063] (a) Detection temperature: 37°C

[0064] (b) Detection wavelength: the main wavelength is 570nm;

[0065] (c) Reaction time: 8 minutes, wherein the incubation time is 5 minutes, the reaction time is 10 seconds, and the detection time is 3 minutes.

[0066] 3. Detection steps (all completed in t...

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Abstract

A D-dimer detection reagent kit, the kit body of the reagent kit comprises two reagents inside: reagent R1: buffer solution, polyethylene glycol, sodium azide, sodium ethylene diamine tetracetate, and bovine serum albumin; reagent R2: buffer solution, latex particles bound with D-dimer monoclonal antibody, Tween-20, sodium azide, sodium ethylene diamine tetracetate, and bovine serum albumin. Mixing a sample with the reagents in a specific volumetric ratio to conduct a series of reactions, placing the reactant under a semi / full automatic biochemical analyzer, and detecting the change speed of light absorbance at the position of 570nm dominant wavelength, thus the concentration of D-dimer can be figured out. The reagent kit has the advantages of accuracy, stability and convenience.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a detection kit for detecting human D dimer. Background technique [0002] The fibrinolytic system is the most important anticoagulant system in the human body. When a fibrin clot is formed, the process of fibrinolysis begins, and dissolves the fibrin clot to form various soluble fragments, D-dimer is one of the most important soluble fragments. [0003] The fibrinolytic system of the human body plays an important role in maintaining the normal permeability of blood vessel walls, maintaining blood flow and tissue repair. Increased levels of D-dimer plasma indicate the presence of secondary fibrinolytic processes. Thrombolytic therapy refers to the use of drugs to activate the fibrinolytic system. Generally, a plasminogen activator such as urine enzyme, streptokinase or tissue-type plasminogen activator is put in to generate a large amount of plasminogen, thereby accelerati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 谢兵
Owner 乐普(北京)诊断技术股份有限公司
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