Method and computer to support identification of keratinocytes
A technology of keratinocytes and computers, applied in the field of supporting the identification of keratinocytes and computers, can solve problems that have not been discovered, and achieve the effect of cost reduction
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Embodiment 1
[0058] The method according to the invention for visually displaying an image of an unstained keratinocyte sample without the use of a liquid dye was tested for the accuracy of the visual assessment. Keratinocyte samples were collected from the skin of three women with different skin conditions. Utilize Adobe Photoshop (registered trademark) of Adobe System Company, according to the above-mentioned color adjustment processing of the present invention, each unstained keratinocyte sample image is displayed (see figure 1 ), and the image is RGB-converted using the above-mentioned RGB conversion chart, thereby obtaining an image displayed in pseudo-color (see image 3 ). Next, each keratinocyte sample was stained with gentian violet and brilliant green, and a magnified image picture thereof was acquired as in the conventional method. Tests were performed on images displayed in pseudo-color and enlarged image pictures dyed into gentian violet and brilliant green (see Patent Docum...
Embodiment 2
[0063] In Example 1, the number of people to be tested was increased to 104, and the measurement accuracy of the present invention was specified. Using Mitani's WinROOF to measure the area of keratinocytes, and according to the standardized evaluation system, three trained evaluators (evaluators) visually evaluated the degree of exfoliation and arrangement regularity on a 5-point scale and a 4-point scale .
[0064] Between the magnified image picture of the present invention and the magnified image picture of the conventional method, correlation coefficients (r) in terms of arrangement regularity, area, and exfoliation degree of keratinocytes were obtained as 0.932, 0.966, and 0.945, respectively. These correlation coefficients demonstrate significant and strong correlations between images. Considering that these images are based on different keratinocyte samples collected from adjacent areas, it can be considered that these images exhibit a high degree of compatibility, i...
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