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Solid composite accelerant for low-temperature methane and preparation method thereof

A composite accelerator and solid technology, applied in the field of agricultural microorganism application, can solve the problems of biogas production in a biogas digester in winter, decomposition of straw can not solve the problem of biogas production in a biogas digester in winter, etc., and achieves the effect of a simple use method.

Inactive Publication Date: 2014-12-17
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the current state of the art that focusing on decomposing straws in the prior art cannot solve the problem of biogas production in user biogas pits in winter, and the compound bacterial agent can only be used in the pretreatment of straws in winter, and cannot be directly put into biogas pits to promote biogas generation; the purpose of the present invention To provide a high-efficiency solid composite microbial bacterial agent suitable for low-temperature biogas production and its preparation method, which solves the problem of biogas production in biogas pits in winter, and can be directly put into biogas pits to promote biogas generation in winter

Method used

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  • Solid composite accelerant for low-temperature methane and preparation method thereof

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Effect test

Embodiment 1

[0026] Example 1: Preparation of Low Temperature Biogas Solid Composite Accelerator

[0027] The present invention is used for the preparation method of low-temperature biogas solid composite accelerator, comprises the following steps:

[0028] (1) Select Bacillus pumilus CICC10440, Lactococcus CICC20711, Pseudomonas ( Pseudomonas sp. ) CGMCC No.4764 and Pantoea agglomerans ( Pantoea agglomerans ) CGMCC No.4765, respectively purify rejuvenation, and obtain fresh pure culture fermentation broth.

[0029] (2) Mix the four pure culture fermentation broths in step (1) according to the mass ratio of 1:1:1:1 to make a liquid composite microbial agent.

[0030] (3) Mix the liquid compound bacterial agent in step (2) with soybean meal and bran of equal mass and mix well, and the soybean meal and bran are mixed in a weight ratio of 1:1.

[0031] (4) Spread the mixture prepared in step (3) in a cool and dry environment to dry in the shade.

[0032] (5) 6%-36% ferric chloride and...

Embodiment 2

[0035] Example 2: Preparation of solid composite microbial agent for biogas low-temperature fermentation

[0036] Bacillus pumilus CICC10440, lactis streptococcus CICC20711, Pseudomonas ( Pseudomonas sp. ) CGMCC No.4764 and Pantoea agglomerans ( Pantoea agglomerans ) CGMCC No.4765 original slant strains of four kinds of bacteria were used as the starting point, and single colonies were obtained by streaking on LB plates, and robust and typical single colonies were picked and transferred to slant surfaces for rejuvenation. Cultivate at 200°C for 24 hours, fill 7 liters of material in a 10 liter fully automatic fermenter, inoculate a liquid shaker flask after sterilization, check under the microscope after 48 hours for no pollution, put it into the tank, and get 7 liters of bacterial liquid; obtain the other two in the same way The bacterial liquid of the strain is mixed with a total of 28 liters. Weigh 28 kg of soybean meal and bran to make a heap in the middle of the site...

Embodiment 3

[0042] Example 3: Low-temperature biogas solid composite accelerator applied to 5 liters of biogas fermentation

[0043] Weigh 1.5 kg of powdered sheep manure, put it into a 5-liter biogas fermentation device, add 3.5 liters of water, mix well, seal it, and start collecting gas production after seven days at a temperature of 10°C-25°C. After 100 days, the gas production starts to decline , add 2.5 grams of the low-temperature biogas solid composite accelerator of the above-mentioned embodiment, mix well, continue to detect the gas production, and set up a blank control group at the same time; see the attached figure 1 shown.

[0044] Weigh 1.5 kg of powdered sheep manure, put it into a 5 liter biogas fermentation device, add 3.5 liters of water, mix well, seal it, collect gas production after seven days, and add 2.5 grams of the above solid composite bacterial agent when the gas production starts to decline after 100 days , mix well, continue to detect the gas production, ...

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Abstract

The invention discloses solid composite accelerant for low-temperature methane and a preparation method thereof. The preparation method includes selecting Bacillus pumilus CICC10440, Streptococcus lactis CICC20711, Pseudomonas sp. CGMCC No. 4764 and Pantoea agglomerans CGMCC No. 4765, performing purification and rejuvenation to each of the four bacteria to obtain fresh pure culture fermentation liquors, mixing the four fermentation liquors according to a mass ratio of 1:1:1:1, well mixing the mixed liquor with soybean meal and bran which are equal in mass of the mixed liquor, spreading mixture in a shady and dry environment for drying in shade, adding 6-36% of ferric trichloride and 1.2-7.2% of nickel chloride, and well mixing to obtain the solid composite accelerant, wherein the soybean meal and the bran are mixed according to a weight ratio of 1:1. The solid composite accelerant is applicable to low-temperature methane and is worthy of wide application.

Description

technical field [0001] The present invention relates to the technical field of agricultural microorganism application, in particular to the technical field of a solid composite microbial agent for improving the level of biogas production under low temperature conditions using cattle and sheep dung and straw as raw materials and a preparation method thereof. Background technique [0002] Biogas belongs to the ground biomass renewable energy, and generally contains methane (CH 4 ) 60%-70%, carbon dioxide (CO 2 )25%-40%, H 2 S, N 2 , CO, H 2 It accounts for about 5%, mainly through bacterial fermentation to reduce the organic matter of plant straw and livestock manure into gas; the thermal efficiency of biogas is relatively stable, easy to use, and its technical economy is second only to liquefied petroleum gas; the preparation of biogas is mainly through bacterial fermentation The anaerobic fermentation process that reduces the organic matter of plant straw and livestock m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P5/02C02F11/04C12R1/07C12R1/46C12R1/38C12R1/01
CPCY02E50/343Y02E50/30
Inventor 陈竞冯蕾杨新平马彩雯史慧锋唐娴秦新政王国强
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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