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Method for extracting tremella polysaccharide and tremella protein

A technology of tremella polysaccharide and tremella protein is applied in the field of bioengineering to achieve the effects of easy engineering amplification, continuous realization and good economic effect

Inactive Publication Date: 2014-05-07
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The method of synchronously extracting tremella polysaccharide and tremella protein using two-phase system has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The strain used in this example is Tremella fuciformis CICC50180, purchased from China Industrial Microorganism Culture Collection and Management Center.

[0042] A method for extracting tremella polysaccharide and tremella protein using a two-phase system, comprising the following steps:

[0043] (1) Inoculate Tremella fungus on the slant medium, activate and cultivate it at 25°C for 7 days, then wash the spores and mycelium on the slant medium with 1wt% saline, and insert it into 10mL liquid seed medium in 150r / min and 25°C for 4 days to produce liquid seeds; put the liquid seeds into the liquid fermentation medium at an inoculum size of 10wt%, and cultivate them for 5 days at a temperature of 25°C and 180r / min to produce get fermentation broth;

[0044] (2) Concentrate the fermented liquid prepared in step (1) through an ultrafiltration membrane with a molecular weight cut-off of 4000 Dal under the condition of 0.2 MPa, and collect the retentate, which is a concentr...

Embodiment 2

[0053] Utilize the method for extracting Tremella polysaccharide and protein as described in Example 1, the difference is:

[0054] In step (3), 100 mL of disodium hydrogen phosphate aqueous solution was prepared, and polyethylene glycol was added to the solution so that the mass percent concentration of disodium hydrogen phosphate in the total system was 13%, and the concentration of polyethylene glycol in the total system was The mass percentage concentration is 27%, and the mixture is evenly mixed to obtain a polyethylene glycol / disodium hydrogen phosphate two-phase aqueous system.

[0055] Tremella polysaccharide product 3.25g was obtained, the polysaccharide content was determined to be 69.24%; tremella protein 0.37g was obtained.

Embodiment 3

[0057] Utilize the method for extracting Tremella polysaccharide and protein as described in Example 1, the difference is:

[0058] In step (3), sodium chloride and polyethylene glycol were added to 100 mL of water to establish a polyethylene glycol / sodium chloride two-phase aqueous system, so that the mass concentration of polyethylene glycol in the total system was 13%, The mass concentration of sodium chloride in the total system is 27%;

[0059] In step (4), add the prepared polyethylene glycol / sodium chloride two-phase aqueous system to the prepared tremella spore crushing liquid at a volume ratio of 1:1.5, and stir and extract.

[0060] 3.68g tremella polysaccharide product was obtained, and the polysaccharide content was determined to be 74.67%; 0.29g tremella protein product was obtained.

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Abstract

The method relates to a method for extracting tremella polysaccharide and tremella protein. The method comprises the steps of (1) activated culture and fermentation culture of tremella fuciformis berk bacteria; (2) concentration with an ultrafiltration membrane and fragmentation tremella fuciformis berk spores; (3) preparation of a polyethylene glycol / inorganic salt aqueous two-phase system; (4) extraction of the polyethylene glycol / inorganic salt aqueous two-phase system; (5) preparation of the tremella polysaccharide via freeze drying; and (6) second extraction of the polyethylene glycol / inorganic salt aqueous two-phase system, filtration with the ultrafiltration membrane and preparation the tremella protein via freeze drying. The method can realize continuous and large-scale production of the tremella fuciformis berk spores and extraction of the tremella polysaccharide, and is convenient for industrialized production of the tremella polysaccharide. The obtained product has not only intercellular polysaccharide of the tremella fuciformis berk spores but also extracellular polysaccharide produced by the liquid fermentation of the tremella fuciformis berk spores, thereby increasing the yield of the tremella polysaccharide. Besides, the tremella polysaccharide and the tremella protein can be separated at the same time, so that the disadvantages of complex operations and pollutions of organic solvents in a conventional sevag method can be prevented; and the purity of the polysaccharide can be increased.

Description

technical field [0001] The invention relates to a method for extracting tremella polysaccharide and tremella protein, in particular to a method for extracting tremella polysaccharide and tremella protein using a two-phase extraction system, and belongs to the technical field of bioengineering. Background technique [0002] White fungus (Tremella fuciformis Berk) is an edible and medicinal fungus, rich in polysaccharides and proteins and other substances. Tremella polysaccharide is the main active substance of Tremella, including five types of acid heteropolysaccharides, neutral heteropolysaccharides, cell wall polysaccharides, exopolysaccharides and acid oligosaccharides. Tremella polysaccharide has various physiological functions such as improving human immunity, anti-tumor, anti-aging, lowering blood sugar and blood fat; Tremella protein contains 17 kinds of amino acids, which can provide 3 / 4 of the amino acids necessary for the human body. Tremella polysaccharide has bee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00C07K1/14C12R1/645
Inventor 董永胜
Owner QILU UNIV OF TECH
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