Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing microspheres through oil in nano-particle suspension and sustained-release microspheres

A nanoparticle and suspension technology, applied in the direction of freeze-drying delivery, powder delivery, drug combination, etc., can solve the problems of local microencapsulation and inflammation that cannot overcome the encapsulation rate, so as to reduce inflammation and microencapsulation, enhance The effect of cell adhesion

Inactive Publication Date: 2014-08-27
SHANGHAI JIAOTONG UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing microspheres in nanoparticle suspension oil-in-oil, to solve the problem that the microspheres in the prior art cannot overcome the local microencapsulation and inflammation caused by low encapsulation efficiency and hydrophobic surface. technical issues
[0004] Another object of the present invention is to provide a sustained-release microsphere to solve the technical problems that the microspheres in the prior art cannot overcome the low encapsulation rate and the local microencapsulation and inflammation caused by the hydrophobic surface

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing microspheres through oil in nano-particle suspension and sustained-release microspheres
  • Method for preparing microspheres through oil in nano-particle suspension and sustained-release microspheres
  • Method for preparing microspheres through oil in nano-particle suspension and sustained-release microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Preparation of doxorubicin polylactic-glycolic acid (PLGA) microspheres with antibacterial and anticancer effects

[0054] (1) Dissolve 20mg of doxorubicin in 0.5ml of dimethyl sulfoxide (DMSO);

[0055] (2) Sonicate the above drug solution and PLGA dichloromethane solution with a percentage concentration of 0.1% at a weight ratio of 1:9 for 1-5 minutes to form a uniform liquid;

[0056] (3) Add the liquid obtained in step (2) dropwise to the suspension of titanium dioxide nanoparticles with a concentration of 1% by weight or the suspension of nanoparticles and PVA surfactant with a concentration of 20% by weight and stir, Vortex or sonicate for 0.1-5 minutes to form double emulsion (O / S) emulsion;

[0057] (4) Add the double emulsion of step (3) dropwise to 1000ml sodium chloride solution with a concentration of 5% (w / w) and solidify for 2 hours;

[0058] (5) The microspheres obtained in the step (4) are collected by centrifugation, washed with water for 3-...

Embodiment 2

[0060] Example 2 Preparation of Exenatide Polylactic-Glycolic Acid (PLGA) Microspheres

[0061] (1) 20 mg and 10 mg of polyethylene glycol (PEG molecular weight 8000) exenatide dissolved in 0.5 ml of dimethyl sulfoxide (DMSO);

[0062] (2) Sonicate the above drug solution and PLGA dichloromethane solutions of different molecular weights and models with a weight ratio of 1:9 for 1-5 minutes to form a uniform liquid;

[0063] (3) Add the emulsion obtained in step (2) dropwise to the suspension of hydroxyapatite nanoparticles with a concentration of 60% by weight or the suspension of nanoparticles and PVA surfactant with a concentration of 30% by weight And stir, vortex or sonicate for 0.1-5 minutes to form (O / S) emulsion;

[0064] (4) Add the double emulsion of step (3) dropwise to 1000ml sodium chloride solution with a concentration of 5% (w / w) and solidify for 2 hours;

[0065] (5) The microspheres obtained in the step (4) are collected by centrifugation, washed with water f...

Embodiment 3

[0067] Example 3 Preparation of octreotide polylactic-glycolic acid (PLGA) microspheres

[0068] (1) 20mg octreotide dissolved in 0.5ml dimethyl sulfoxide (DMSO) solution;

[0069] (2) Sonicate the above drug solution and PLGA dichloromethane solution with a percentage concentration of 20% in a weight ratio of 1:9 for 1-5 minutes to form a uniform liquid;

[0070] (3) Add the liquid obtained in step (2) dropwise to the suspension of hydroxyapatite nanoparticles with a concentration of 80% by weight or the suspension of nanoparticles and PVA surfactant with a concentration of 50% by weight And stir, vortex or sonicate for 0.1-5 minutes to form (O / S) emulsion;

[0071] (4) Add the double emulsion of step (3) dropwise to 1000ml sodium chloride solution with a concentration of 5% (w / w) and solidify for 2 hours;

[0072] (5) The microspheres obtained in the step (4) are collected by centrifugation, washed with water for 3-5 times, and freeze-dried to obtain the microspheres.

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing microspheres and particularly relates to a method for preparing microspheres through oil in nano-particle suspension and sustained-release microspheres. The method includes that step one, embedded medicine ingredients are prepared to a medicine oil solution; step two, the medicine oil solution is added into a polymer organic solution, namely the medicine oil solution is added into an oil phase, and a suspension is formed through uniformly mixing; step three, the suspension prepared in the step two is dropwise added into a suspension containing nano-particles, and the microspheres are formed through stirring, whirling or ultrasound; step four, the microspheres prepared in the step three are dispersed into an aqueous phase for curing; and step five, the microspheres are collected in a centrifuged mode, and freeze-drying is performed to obtain the microspheres with surfaces self assembled with the nano-particles. Compared with methods for preparing microspheres in prior art, the method for preparing the microspheres through oil in nano-particle suspension has the advantages that a layer of nano-particles is assembled on the surfaces of the microspheres, and the microspheres with surfaces assembled with the nano-particles have effects of enhancing cell adhesion and reducing inflammation and microencapsulation caused by local peracid and a hydrophobic material.

Description

technical field [0001] The invention relates to a method for preparing microspheres, in particular to a method for preparing microspheres in oil-in-nano particle suspension and sustained-release microspheres. Background technique [0002] In the pharmaceutical industry, from drug discovery to clinical application, the last link is drug preparation. Among them, some drugs require long-term administration to be cured; others require targeted and other local administration. To achieve these goals, raw materials must be prepared into corresponding dosage forms. For example, drugs that require long-term administration but have a short half-life in the body should be prepared in PLA (polylactic acid) dosage form; for the treatment of some tumors, some drugs need to be targeted to the lesion, such as embolization microsphere preparations targeting tumor blood vessels Wait. After searching the prior art documents, it was found that "Double walled PLA / PLGA microspheres: encapsulat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61P35/00
Inventor 袁伟恩金拓吴飞廖美玲张敏吴克沈炎冰
Owner SHANGHAI JIAOTONG UNIV