Sugarcane sucrose transport protein SoERD6 (early responsive to dehydration) gene and encoding protein sequence thereof

Abstract

The invention discloses a sugarcane sucrose transport protein SoERD6 (early responsive to dehydration) gene and an encoding protein sequence thereof. On the basis of performing comparative analysis on a full-length sequence of all plant sugarcane sucrose transport protein SoERD6 genes, a primer is designed at a conserved region of the plant sugarcane sucrose transport protein SoERD6 gene, the total RNA (Ribonucleic Acid) is extracted from sugarcane puerile leaves, subjected to inverse transcription, amplified by normal PCR (polymerase chain reaction), and cloned to the full-length cDNA sequence of the sugarcane sucrose transport protein SoERD6 genes by combining an RACE (rapid-amplification of cDNA ends) PCR technique. With the adoption of the gene, not only is the basis provided for researching the transcription and expression mechanism of the sugarcane sucrose transport protein SoERD6 gene and further studying the transportation of sucrose in a sugarcane body and an accumulation mechanism in a sugarcane stem, but also a purified protein with biological activity can be obtained via an amino acid sequence; and the sugarcane sucrose transport protein SoERD6 gene is used for studying the biological function of the sugarcane sucrose transport protein and improving sugarcane high-sugar variety.

Description

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AI Technical Summary

Benefits of technology

This patented method allows researchers to investigate how sugars move within plants without losing their ability or affecting them too much by analyzing its structure. By comparing different sequences from Sugarcanes biosynthesis (SSB) it becomes clear that certain parts are important for plant growth while others may have negative side effect on those areas.

Problems solved by technology

This patent describes different types of sugars called sorghum syrupes, including glutanum crop species like sweet corn, wholly grapefruit, citrus fruits, tomato seeds, etc., along with other sources of these compounds. These natural resources provide various benefits when they contribute towards agricultural production processes. They help regulate soil water content during vegetative phases of flowering stages, allowing them to absorb excess moisture without causing issues associated with dryness. Additionally, certain enzymatic activities within the biosynthesis machinergysome system allow sucrose molecules to enter the endosperm where they act as osmosis pumps. Overall, all these technical effects make sugarcutter machines essential components necessary for efficient use of sweetenings throughout the worldwide food chain.

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