Antioxidant active peptide and preparation method by ultrasonic electrophoresis separation

Abstract

The invention discloses an antioxidant active peptide based on wheat germ protein zymolyte and a preparation method by ultrasonic electrophoresis separation. The preparation method comprises the following key stages of raw materials: 1, mixture of wheat germ and water; 2, compound raw materials; and 3, electrophoresis raw materials. By the method, the raw materials with low cost can be used, and antioxidant active peptide with consistent molecular weight is obtained; and secondary purification is performed by using a semipermeable membrane so as to obtain the antioxidant active peptide with high purity.

Description

technical field [0001] The invention relates to a method for separating antioxidant active peptides in the field of bioengineering, in particular to an antioxidant active peptide based on enzymatic hydrolyzate of wheat germ protein and a preparation method thereof by ultrasonic electrophoresis separation. Background technique [0002] In recent years, more and more attention has been paid to the research and development of the separation of antioxidant active peptides, especially the problem of low-cost and high-purity separation. The traditional isolation of antioxidant active peptides is enriched by filtration, precipitation and other methods. The process is complicated and the purity of the finished product is limited, which is limited in practical application. [0003] Chinese application number CN200810064296.8, application publication number CN101284872, the patent discloses an antioxidant active peptide and its preparation method, the invention uses the microorganism ...

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Problems solved by technology

However, the purity of the finished product obtained by this method is low, and the polypeptide content contained is relatively high.

[0005] In summary, although the separation of antioxidant active peptides h