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Antioxidant active peptide and preparation method by ultrasonic electrophoresis separation

A technology of antioxidant active peptides and ultrasonic electrophoresis, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problem of low purity of finished products, high polypeptide components, and the preparation method of ultrasonic electrophoresis separation of antioxidant active peptides that has not been reported. To achieve high purity, improve the enrichment purity, and speed up the enrichment speed

Inactive Publication Date: 2013-03-27
昆山冠强软件科技有限公司 +1
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  • Abstract
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Problems solved by technology

However, the purity of the finished product obtained by this method is low, and the polypeptide content contained is relatively high.
[0005] In summary, although the separation of antioxidant active peptides has been studied to a certain extent, there is no report in the literature on the preparation of antioxidant active peptides based on wheat germ protein hydrolyzate and its ultrasonic electrophoresis separation

Method used

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Embodiment Construction

[0013] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0014] The antioxidant active peptide based on enzymatic hydrolyzate of wheat germ protein and its ultrasonic electrophoresis separation preparation method described in this example include the following key raw material stages: mixture 1 of wheat germ and water, composite raw material 2, and electrophoresis raw material 3.

[0015] In this embodiment, the mixture 1 of wheat germ and water refers to pulverizing the wheat germ at room temperature to obtain a product with a density of 1.01-5.2 grams per cubic centimeter.

[0016] In this embodiment, the composite raw material 2 refers to the raw material prepared by ...

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Abstract

The invention discloses an antioxidant active peptide based on wheat germ protein zymolyte and a preparation method by ultrasonic electrophoresis separation. The preparation method comprises the following key stages of raw materials: 1, mixture of wheat germ and water; 2, compound raw materials; and 3, electrophoresis raw materials. By the method, the raw materials with low cost can be used, and antioxidant active peptide with consistent molecular weight is obtained; and secondary purification is performed by using a semipermeable membrane so as to obtain the antioxidant active peptide with high purity.

Description

technical field [0001] The invention relates to a method for separating antioxidant active peptides in the field of bioengineering, in particular to an antioxidant active peptide based on enzymatic hydrolyzate of wheat germ protein and a preparation method thereof by ultrasonic electrophoresis separation. Background technique [0002] In recent years, more and more attention has been paid to the research and development of the separation of antioxidant active peptides, especially the problem of low-cost and high-purity separation. The traditional isolation of antioxidant active peptides is enriched by filtration, precipitation and other methods. The process is complicated and the purity of the finished product is limited, which is limited in practical application. [0003] Chinese application number CN200810064296.8, application publication number CN101284872, the patent discloses an antioxidant active peptide and its preparation method, the invention uses the microorganism ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/26
Inventor 康晓洋
Owner 昆山冠强软件科技有限公司
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