Gagal transgenic soybean and choosing method of rhizosphere soil microorganism community diversity measurement index of receptor of gagal transgenic soybean

A technology of microbial community and rhizosphere soil, applied in the field of metric index selection, can solve rare problems
CN103014149AInactive Publication Date: 2013-04-03JIANGSU ACAD OF FORESTRY
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
JIANGSU ACAD OF FORESTRY
Publication Date
2013-04-03
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention relates to a gagal transgenic soybean and a choosing method of the rhizosphere soil microorganism community diversity measurement index of the receptor of the gagal transgenic soybean, and particularly relates to a choosing technology of the rhizosphere soil microorganism community diversity measurement index of a transgenic soybean planted in a field, relating to the technical field of soil microbiology research, and solving the technical problem that superior diversity indices are chosen through a scientific method to measure the structural, hereditary and functional diversity of soil microorganism communities. The technical scheme is as follows: the choosing method is characterized in that the superior diversity indices for measuring various indices are chosen according to the measurement results of different diversity indices on the gagal transgenic soybean and the rhizosphere soil microorganism community diversity of the receptor of the gagal transgenic soybean and the difference significance degree of the same diversity index on the measurement values of the PLFA (Phosphor Lipid Fatty Acid), DGGE (Denaturing Gradient Gel Electrophoresis) and BIOLOG levels of the soil microorganism communities combined with the significant correlation of the rhizosphere soil microorganism communities among diversity level research indices. The choosing method disclosed by the invention is mainly used for measuring the rhizosphere soil microorganism community diversity of other transgenic soybeans.
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Description

1: Technical field

[0001] The invention belongs to the technical field of soil microbiology research, and in particular relates to the selection technology of the measurement index of the microbial community diversity in the rhizosphere soil of transgenic soybeans under the field planting mode. Two: background technology

[0002] At present, the research methods of soil microbial community diversity mainly include PLFA microbial lipid analysis method, BIOLOG microplate method, and DNA fingerprint analysis method based on PCR-amplified rRNA, etc. Scientists usually use the change of PLFA type and quantity to explore the change of soil microbial community structure diversity, and use PCR-DGGE analysis to display the amplification of soil bacterial 16SrDNA and the increase and decrease of bacterial species to explore the genetic diversity of soil microorganisms. To explore the soil culturable microbial community (including germ Changes in functional diversity of gram-negative ...

Claims

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