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Gagal transgenic soybean and choosing method of rhizosphere soil microorganism community diversity measurement index of receptor of gagal transgenic soybean

A technology of microbial community and rhizosphere soil, applied in the field of metric index selection, can solve rare problems

Inactive Publication Date: 2013-04-03
JIANGSU ACAD OF FORESTRY
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Problems solved by technology

Although the above literature emphasizes the correlation of different diversity indices in describing the ecological significance, the extensive experimental data of soil microbial community BIOLOG (-GN, -GP, -FF and -ECO) plates, PCR-DGGE and PLFA Using these diversity indexes for analysis and processing, and finally determining the available measurement indexes of the dominance of each soil index, it is rare so far

Method used

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  • Gagal transgenic soybean and choosing method of rhizosphere soil microorganism community diversity measurement index of receptor of gagal transgenic soybean
  • Gagal transgenic soybean and choosing method of rhizosphere soil microorganism community diversity measurement index of receptor of gagal transgenic soybean
  • Gagal transgenic soybean and choosing method of rhizosphere soil microorganism community diversity measurement index of receptor of gagal transgenic soybean

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] Table 1 Soybean varieties used in the test

[0011]

[0012] As shown in Table 1, different lines of transgenic soybeans were used in the experiment, and their non-transgenic recipient varieties were used as controls. Three replicates were set up for each sample, and they were planted in the Mid-Autumn Festival in the field net room in 2009. The microbial community PLFA, PCR-DGGE and Biolog (GN2, GP2, FF and ECO plates) data obtained from soybean rhizosphere soil at flowering stage were used to measure the diversity index. The index formula for measuring soil microbial community diversity is shown in Table 2.

[0013] Table 2 Formula of soil microbial community diversity index

[0014]

[0015] All soil samples were subjected to 3 repeated independent experimental treatments. All data were processed using Excel and SPSS19.0 software for statistical analysis, and the differences between treatments were compared using ANOVA. The original Biolog data is the AWCD ...

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Abstract

The invention relates to a gagal transgenic soybean and a choosing method of the rhizosphere soil microorganism community diversity measurement index of the receptor of the gagal transgenic soybean, and particularly relates to a choosing technology of the rhizosphere soil microorganism community diversity measurement index of a transgenic soybean planted in a field, relating to the technical field of soil microbiology research, and solving the technical problem that superior diversity indices are chosen through a scientific method to measure the structural, hereditary and functional diversity of soil microorganism communities. The technical scheme is as follows: the choosing method is characterized in that the superior diversity indices for measuring various indices are chosen according to the measurement results of different diversity indices on the gagal transgenic soybean and the rhizosphere soil microorganism community diversity of the receptor of the gagal transgenic soybean and the difference significance degree of the same diversity index on the measurement values of the PLFA (Phosphor Lipid Fatty Acid), DGGE (Denaturing Gradient Gel Electrophoresis) and BIOLOG levels of the soil microorganism communities combined with the significant correlation of the rhizosphere soil microorganism communities among diversity level research indices. The choosing method disclosed by the invention is mainly used for measuring the rhizosphere soil microorganism community diversity of other transgenic soybeans.

Description

1: Technical field [0001] The invention belongs to the technical field of soil microbiology research, and in particular relates to the selection technology of the measurement index of the microbial community diversity in the rhizosphere soil of transgenic soybeans under the field planting mode. Two: background technology [0002] At present, the research methods of soil microbial community diversity mainly include PLFA microbial lipid analysis method, BIOLOG microplate method, and DNA fingerprint analysis method based on PCR-amplified rRNA, etc. Scientists usually use the change of PLFA type and quantity to explore the change of soil microbial community structure diversity, and use PCR-DGGE analysis to display the amplification of soil bacterial 16SrDNA and the increase and decrease of bacterial species to explore the genetic diversity of soil microorganisms. To explore the soil culturable microbial community (including germ Changes in functional diversity of gram-negative ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 刘根林
Owner JIANGSU ACAD OF FORESTRY
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