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Myocardial infarction early-warning colloidal gold kit and preparation method thereof

A technology of colloidal gold and test area, which is applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as indicators that cannot be early warning, and achieve the effects of sensitive detection, accurate results, and reduction of clutter.

Active Publication Date: 2013-04-17
同昕生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Short-term and reversible ischemic attacks will not lead to elevated blood levels of these markers, so they are all markers of myocardial necrosis and cannot be used as early warning indicators

Method used

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  • Myocardial infarction early-warning colloidal gold kit and preparation method thereof
  • Myocardial infarction early-warning colloidal gold kit and preparation method thereof
  • Myocardial infarction early-warning colloidal gold kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, immunocolloidal gold chromatography detects Lp-PLA 2

[0025] The present invention uses double-antibody sandwich method to detect Lp-PLA in human serum or plasma 2 , using genetically engineered and purified Lp-PLA 2 The monoclonal antibody made of protein is coated with nitrocellulose membrane, another monoclonal antibody or polyclonal antibody is labeled with colloidal gold and spread evenly on glass fiber paper, and the detection of Lp-PLA is achieved by using paper chromatography technology and immunogold color reaction 2 the goal of. Features: The entire operation time is about 20 minutes, and it can be operated by professional laboratory test personnel in hospitals or independently by testers.

[0026] Kit composition of the present invention: Lp-PLA 2 Test card, 1 instruction manual, 1 bottle of sample diluent

[0027] Step 1. Preparation of various reagents

[0028] 1. Sample diluent: Dissolve 0.2g potassium chloride (KC1) (Beijing Chemical R...

Embodiment 2

[0058] Embodiment 2, comparative test

[0059] Refer to test strip: test strip 1 prepared in embodiment 1, contrast 1~5.

[0060] Comparative experiment 1: with known Lp-PLA 2 Serum samples with a concentration above 235ng / ml were detected according to the detection steps described in Example 1, and the detection results of test strip 1 and control 1 were as follows image 3

[0061] Comparative experiment 2: with known Lp-PLA 2 Serum samples with a concentration above 235ng / ml were detected according to the detection steps described in Example 1. The detection results of test strip 1 and controls 2-5 were as follows: Figure 4 :

Embodiment 3

[0062] Embodiment 3, immunocolloidal gold diafiltration method detects Lp-PLA 2

[0063] The present invention applies the principle of enzyme immunological double-antibody sandwich method to detect Lp-PLA in human serum or plasma by using nano colloidal gold rapid spot immunofiltration technology 2 , using genetically engineered and purified Lp-PLA 2 The monoclonal antibody made of protein is coated with nitrocellulose membrane, another monoclonal antibody or polyclonal antibody is labeled with colloidal gold to prepare a conjugate reagent, and the detection of Lp-PLA is achieved by using the diafiltration technique and the color reaction of immunogold 2 the goal of. The design principle is that the immune reaction is carried out by vertically penetrating the nitrocellulose membrane immobilized with the ligand. The nitrocellulose membrane (NC membrane) is used as the carrier, and the filterability of the microporous membrane is used to make the antigen-antibody reaction and ...

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Abstract

The invention provides a myocardial infarction early-warning colloidal gold kit and a preparation method thereof and relates to a medical detection device. According to the myocardial infarction early-warning colloidal gold kit and the preparation method thereof, the preparation method comprises the following steps of: preparing a colloidal gold mark protein solution of a colloidal gold mark protein related phospholipase A2 monoclonal antibody and detecting a reaction carrier. The preparation method is characterized in that PEG20000 (Poly Ethylene Glycol 20000) with the concentration of 0.24mg / ml, BSA (Bull Serum Albumin) with the concentration of 1.5% (w / w), cane sugar with the concentration of 3% (w / w), mycose with the concentration of 0.5% (w / w), casein with the concentration of 1% (w / w) and sodium dodecyl sulfate SDS are added into the colloidal gold mark protein solution. According to the improvement of the preparation method disclosed by the invention, two kinds of colloidal gold detection kits capable of rapidly detecting whole-blood lipoprotein related phospholipase A2 (Lp-PLA2) are provided; and the invention further provides the colloidal gold mark protein solution for optimizing the property of the kit.

Description

technical field [0001] The invention relates to a medical prediagnosis reagent, in particular to a colloidal gold kit for early warning of myocardial infarction and a preparation method thereof. Background technique [0002] Myocardial infarction (MI or acute myocardial infarction, AMI) often occurs when the coronary arteries of the heart are suddenly blocked, causing a sudden interruption of the supply of part of the heart fluid, resulting in ischemia and oxygen shortage. If not treated in time, it will lead to myocardial cell death. Myocardial infarction is the ultimate outcome of cardiovascular disease (also collectively referred to as coronary heart disease), which is characterized by sudden, non-specific aura symptoms, resulting in high mortality rates due to inadequate treatment. Recent epidemiological data show that about 17 million people die from coronary heart disease every year in the world, and more than half of them die from myocardial infarction. In my country...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/544G01N33/531
Inventor 吴凡焦守恕满慧娟
Owner 同昕生物技术(北京)有限公司
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