Method for detecting tissue cell protein using housekeeping protein

A tissue cell and protein technology, applied in the field of tissue cell protein quantitative detection, can solve the problems of inability to interpret the absolute or relative quantification of the detected protein, the low sensitivity of the gold standard method, and the inability to detect proteins, so as to improve the detection sensitivity and expand the application , high precision effect

Inactive Publication Date: 2016-01-27
苏州海吉亚生物科技有限公司
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Problems solved by technology

[0006] However, the sensitivity of the gold standard method is too low, and the experimental error between different batches is too large. Moreover, the presence or absence of the detected protein can only be judged by color development, and the absolute or relative quantification of the detected protein cannot be interpreted.
Therefore, the existing gold standard method cannot detect the protein in tissue cells at all

Method used

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  • Method for detecting tissue cell protein using housekeeping protein
  • Method for detecting tissue cell protein using housekeeping protein

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Embodiment Construction

[0026] Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0027] see figure 1 , 2 . It should be noted that the diagrams provided in this embodiment are only schematically illustrating the basic idea of ​​the present invention, so only the components related to the present invention are shown in the drawings rather than the number, shape and Dimensional drawing, the type, quantity and proportion of each component can be changed arbitrarily during actual implementation, and the component la...

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Abstract

The invention relates to a method for detecting tissue cell protein by using housekeeping protein. According to the method, housekeeping protein and a monoclonal antibody or a polyclone antibody of protein to be detected are coupled with colloidal gold or colloidal silver respectively, the colloidal gold or silver antibody compound formed after coupling is dripped on a gold marking pad of a test paper; and another monoclonal antibody or polyclone antibody against corresponding housekeeping protein or protein to be detected is fixed at a detection area of the test paper, and the negative or the positive of the protein detected is judged according to the relative calculation ratio of the color of the housekeeping protein on the test paper to the color of the detected protein. The detection method solves the quantitative problem in colloidal gold immunoassay, and widens the application of colloidal gold immunoassay in protein detection; and in addition, the detection method has the advantages of fastness in speed, high accuracy and the like compared with other protein detection.

Description

technical field [0001] The invention relates to a method for quantitative detection of tissue cell protein, in particular to a method for quantitative detection of tissue cell protein by using housekeeping protein. Background technique [0002] In the past tumor treatment, especially chemotherapy, because the drugs have a wide range of effects, while killing tumor cells, they also kill a large number of normal cells, resulting in unbearable toxic side effects. With the rapid progress of medical research, scientists have gradually developed targeted tumor therapy drugs with strong pertinence and less toxic side effects. At present, this kind of targeted therapy has increasingly become the development trend of tumor treatment due to its good therapeutic effect and low toxic and side effects. However, how to quickly and accurately judge the molecular type of a patient's tumor in the clinic, and then determine whether the patient is suitable for a certain targeted therapy is st...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 糜军
Owner 苏州海吉亚生物科技有限公司
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