Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in soil and preparation method thereof

A microfluidic chip, polycyclic aromatic hydrocarbon technology, which is applied in the preparation of test samples, chemical instruments and methods, measurement devices, etc., can solve problems such as no substantial breakthroughs, achieve good application prospects, and shorten detection time. , the effect of reducing the dosage

Inactive Publication Date: 2013-04-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the centrifugal microfluidic chip analysis technology that uses a wafer-shaped chip, uses microfabrication technology to prepare microstructures and microchannels on its surface for analysis, relies on centrifugal force to drive sample microfluidics, and completes the detection of multiple samples at the same time. There have been no substantial breakthroughs in the application fields related to the rapid detection of aromatic hydrocarbons

Method used

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  • Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in soil and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The microstructure and microchannel graphics of the five-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. Microstructures and microchannels were prepared on each layer of polymethyl methacrylate (PMMA) chips by a numerically controlled CNC micromachining system. The chips of each layer were cleaned with tap water and distilled water, and fingerprints, oil stains and other stains remaining on the chip surface were wiped with ethanol. Put 30 mg of the soil sample to be tested in the extraction pool, carefully align, bond, and seal the five-layer chip to make a centrifugal microfluidic chip for detecting polycyclic aromatic hydrocarbons in soil. Fix the centrifugal microfluidic chip on the rotating platform of the centrifuge through a suction cup, and add 100 μL C from the sample injection hole. 2 Cl 4 , mixed at a speed of 150 rpm, after extraction for 10 minutes, increase the speed to 500 rpm, and transfer all th...

Embodiment 2

[0030] The microstructure and microchannel graphics of the five-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. Microstructures and microchannels were prepared on each layer of polycarbonate (PC) chips by a numerically controlled CNC micromachining system. Finally, each layer of chips was cleaned with tap water and distilled water, and residual fingerprints, oil stains and other stains on the chip surface were wiped with ethanol. Put 30 mg of the soil sample to be tested in the extraction pool, carefully align, bond, and seal the five-layer chip to make a centrifugal microfluidic chip for detecting polycyclic aromatic hydrocarbons in soil. Fix the centrifugal microfluidic chip on the rotating platform of the centrifuge through a suction cup, and add 100μLC from the sample injection hole 2 Cl 4 , mixed at a speed of 300 rpm, after extraction for 5 minutes, increase the speed of the centrifuge to 700 rpm, and move all t...

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Abstract

The invention relates to a centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in soil and a preparation method thereof. The chip is a centrifugal micro-fluidic chip with a disc-shaped micro-structure and a micro-channel and is formed by sealing a plurality of layers of chips on which micron level micro-structures and micro-channels are engraved and a double-layer adhesive film, the centrifugal force is taken as the driving force of sample micro-fluid to finish the mixing, extraction and separation processes of a sample to be detected and an extract, and an infrared spectrometer is taken as a detection device to quantificationally or qualitatively detect the content of oil and grease in a sample on the chip. The centrifugal micro-fluidic chip and the preparation method thereof have the benefits that a plurality of groups of samples can be simultaneously treated and detected, the consumption of reagents as well as the samples is less, the integration, the miniaturization and the automation of polycyclic aromatic hydrocarbon detection are realized, the centrifugal micro-fluidic chip has the characteristics of economy, quickness, convenience in carrying and high efficiency, and a brand-new analysis technology platform is provided for conveniently, quickly and accurately detecting the polycyclic aromatic hydrocarbon.

Description

technical field [0001] The invention relates to a microfluidic chip for detecting polycyclic aromatic hydrocarbons in soil and a preparation method thereof. The chip is a disc-shaped centrifugal microfluidic chip, and microstructures and microchannels are prepared on the surface by micromachining technology, and rotated in a centrifuge. Driven by the generated centrifugal force, the process of mixing, extracting, separating and filtering the sample to be tested and the extractant is realized, and the content of polycyclic aromatic hydrocarbons in the sample on the chip is quantitatively or qualitatively detected by infrared spectrometer, which is mainly used in environmental protection and water quality detection, etc. Related areas. Background technique [0002] Polycyclic aromatic hydrocarbons (polycyclic aromatic hydrocarbons) are hydrocarbons containing more than two benzene rings in the molecule, including more than 150 compounds such as naphthalene, anthracene, phenant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N1/40G01N21/35G01N35/00
CPCG01N21/07G01N21/3577
Inventor 王晓东
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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