Benzoquinone derivative from aspergillus aculeatus and application of benzoquinone derivative

A kind of use and drug technology, applied in the field of benzoquinone derivatives, can solve the problems such as drugs that have not yet been seen, and achieve significant anti-tumor activity

Inactive Publication Date: 2014-07-30
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study found that the benzoquinone compounds shown have anti-tumor activity, but there is no report on the chemical structure and cell proliferation inhibitory activity of the compound, so there is no drug related to it in the market

Method used

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  • Benzoquinone derivative from aspergillus aculeatus and application of benzoquinone derivative
  • Benzoquinone derivative from aspergillus aculeatus and application of benzoquinone derivative
  • Benzoquinone derivative from aspergillus aculeatus and application of benzoquinone derivative

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Experimental program
Comparison scheme
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Embodiment 1

[0010] Fermentative production and separation and purification of the compound of embodiment 1

[0011] 1 Fermentation production

[0012] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, get Aspergillus aculeatus ( Aspergillus aculeatus ) IBPT-3 (preserved in China Center for Type Culture Collection on December 24, 2012, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2012544) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 4 days.

[0013] Aspergillus aculeatus ( Aspergillus aculeatus ) Appropriate amount of IBPT-3, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5 , MgSO 4 0.3, NaCl 6.0, constant volume with water] in a 1000mL Erlenmeyer flask, after static culture at 28°C for 30 days, mycelium and fermentation broth wer...

Embodiment 2

[0024] Example 2 Test of anti-tumor activity in vitro

[0025] 1 Experimental samples and experimental methods

[0026] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0027] Cell lines and subculture of cells adopt human lung cancer A549 cell line, leukemia HL-60 cells and K562 cells. All kinds of cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0028] Cell Proliferation Inhibitory Activity Test Method

[0029] Lissamine rhodamine B (SRB) method Take the human lung cancer A549 cells in the logarithmic growth phase, and use fresh RPMI-1640 medium to prepare a density of 2×10 per ml 5 The cell suspension of each cell was inoculated in a 96-well cell cult...

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Abstract

The invention relates to a benzoquinone derivative derived from Aspergillus aculeatus and application thereof. The structural formula of the compound is:. Experiments have proved that the benzoquinone compound has better antitumor activity. It can be used as the preparation of cell proliferation inhibitory drugs or anti-tumor drugs for anti-tumor research.

Description

technical field [0001] The invention relates to a benzoquinone derivative derived from Aspergillus aculeatus and application thereof. Background technique [0002] Aspergillus aculeatus ( Aspergillus aculeatus ) is the source of many biologically active compounds. Previous chemical studies on Aspergillus aculeatus revealed that its secondary metabolites have good antibacterial, antifungal, antiviral, insecticidal, and antitumor activities. These active compounds are classified according to their structure types. It can be divided into peptides, alkaloids, sesquiterpenes, fatty acids, and xanthones. However, the structure of benzoquinone and benzene ring connected by an oxygen bridge is rarely reported in the literature. The inventors have studied and learned that Aspergillus aculeatus ( Aspergillus aculeatus ) The crude extract of the fermentation product of IBPT-3 (preserved in the China Center for Type Culture Collection on December 24, 2012, and the preservation number ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C50/30A61P35/00A61P35/02
Inventor 张其清陈立张维维胡筱方哲翔伍久林
Owner FUZHOU UNIV
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