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Preparation method of voltage-controllable multiplex multichannel sensing paper chip for detecting antibiotic residues by molecular imprinting electroluminescence

A technology of molecular imprinting and antibiotics, which is applied to the detection of antibiotic residues in egg products, meat, and electroluminescence sensors in the field of milk, can solve the problems of low sensitivity and selectivity, expensive reagents, and expensive instruments, and achieve high sensitivity. and detection range, improve sensitivity and accuracy, and reduce detection costs

Inactive Publication Date: 2014-09-03
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006]2. UV-Vis spectrophotometry and fluorescence method have the advantages of simple operation, low cost, and wide linear range, but their sensitivity and selectivity are not high
[0007]3. The sensitivity of liquid chromatography-mass spectrometry is relatively high, but the instrument is expensive and the operation is relatively complicated
[0008]4. Enzyme-linked immunoassay has high sensitivity, but the operation steps are more complicated, but the reagents are expensive and the cost is relatively high
[0009]5. Microbial method has the advantages of low cost and time saving, but it is generally only used in screening experiments
[0010] For the detection and analysis of antibiotic residues in the above methods, there are generally high detection costs, false positive results, complicated detection process, high cost, large amount of reagents, It is not suitable for on-site rapid detection, and the interference of coexisting substances is large, so it cannot meet the needs of actual detection

Method used

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  • Preparation method of voltage-controllable multiplex multichannel sensing paper chip for detecting antibiotic residues by molecular imprinting electroluminescence
  • Preparation method of voltage-controllable multiplex multichannel sensing paper chip for detecting antibiotic residues by molecular imprinting electroluminescence
  • Preparation method of voltage-controllable multiplex multichannel sensing paper chip for detecting antibiotic residues by molecular imprinting electroluminescence

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Example 1 (chloramphenicols, such as Chloramphenicol)

[0057] A method for preparing a voltage-controllable and multiplexed multi-channel sensing paper chip for detecting chloramphenicol residue by molecular imprinting electroluminescence, comprising the following steps:

[0058] (1). Preparation of voltage regulators and multiplexing switches;

[0059] (2). Preparation of printed electrodes on multi-channel paper: first print on A4 paper with a wax printer as attached image 3 The wax pattern shown, and put the A4 paper with the wax pattern in a flat heater or an oven, and heat it at 60-150°C for 0.5-2 minutes, so that the wax melts and soaks through the entire thickness of the paper to form a hydrophobic area ; Using the screen printing method, the carbon working electrode and the Ag / AgCl reference electrode (as attached Figure 4 );

[0060] (3). Select the functional monomer diethylaminoethyl methacrylate (DEAEM) that can synthesize MIPs with chloramphenic...

Embodiment 2

[0074] Example 2 (quinolones, such as rifampicin)

[0075] A method for preparing a voltage-controllable and multiplexed multi-channel sensor paper chip for detecting rifampicin residues by molecular imprinted electroluminescence, comprising the following steps:

[0076] (1). Preparation of voltage regulators and multiplexing switches;

[0077] (2). Preparation of printed electrodes on multi-channel paper: first print on A4 paper with a wax printer as attached image 3 The wax pattern shown, and put the A4 paper with the wax pattern in a flat heater or an oven, and heat it at 60-150°C for 0.5-2 minutes, so that the wax melts and soaks through the entire thickness of the paper to form a hydrophobic area ; Using the screen printing method, the carbon working electrode and the Ag / AgCl reference electrode (as attached Figure 4 );

[0078] (3). Select the functional monomer α-methacrylic acid (MAA) that can synthesize MIPs with rifampicin;

[0079] (4). Template molecule...

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Abstract

The invention discloses a method for preparing a multi-channel sensing paper chip with voltage controllability and multiplex conversion for detecting antibiotic residues by molecular imprinting electroluminescence. Including the following steps: self-made transformers and multiplexing switches; preparation of multi-channel printed electrodes on paper; preparation of molecularly imprinted (MIPs) sols of antibiotic residues; preparation of carbon dots and carbon dot-coated silica microspheres; preparation of graphite Graphene nanomaterials; using electrode surface modification technology to modify graphene and carbon dot-coated silica microspheres and MIPs sols on the surface of multi-channel electrodes on paper. A method for preparing a voltage-controllable and multiplexed multi-channel sensor paper chip for detecting antibiotic residues by molecularly imprinted electroluminescence also includes the following steps, combining the modified electrode with a chemiluminescence analyzer, a transformer and a multiplexer The switch detects antibiotic residues in the sample extract. The paper chip of the invention has strong specificity and high sensitivity, which can reach ng level; and the cost is low. The operation is fast and simple, and the reaction and results are automatically completed and recorded by the instrument.

Description

technical field [0001] The present invention relates to the technical field of antibiotic residue detection, more specifically to the preparation of a multi-channel sensor paper chip with molecularly imprinted electroluminescence detection of antibiotic residue with controllable voltage and multiplexing. Method for detecting antibiotic residues in milk, meat and egg products by electroluminescence sensor. Background technique [0002] Antibiotics are secondary metabolites of microorganisms that can kill or inhibit other microorganisms in the metabolic process. Since the 1950s, they have been widely used in the livestock and poultry industry. Their main uses include animal disease treatment, animal growth Regulators and improve feed utilization efficiency. Antibiotics, as drugs and non-drugs, play an important role in the development of livestock and poultry farming, and their use in many countries is also increasing. The use of antibiotics has made a considerable contribut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/66
Inventor 李念强张彦于京华葛慎光葛磊颜梅王盼盼楚成超苏敏
Owner UNIV OF JINAN