Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of gel containing fibroblast growth factor-1 modified body and application thereof for treating diabetic foot

A technology of fibroblasts and human fibroblasts, applied in the field of biomedicine, can solve problems such as reducing the quality of life of patients, reducing anti-infection ability, and immune dysfunction, and achieve the effects of increasing clinical application, promoting effective healing, and delaying release

Active Publication Date: 2013-05-08
安徽鑫华坤生物工程有限公司
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ischemia and nerve injury reduce local tissue healing ability and anti-infection ability, so the wound is not easy to heal
In terms of pathophysiology, it mainly manifests as abnormal metabolism of wound tissue cells and immune dysfunction, especially the dysfunction of local macrophages and mast cells in the wound, accompanied by impaired growth factor function in the wound microenvironment, and local microcirculation perfusion disturbance in the wound. Tissue hypoxia, etc., resulting in long-lasting wounds and ulcers that are difficult to heal, often leading to gangrene or amputation, which greatly reduces the quality of life of patients, and the treatment is quite difficult, the cycle is long, and the medical expenses are high. great pain and heavy burden

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of gel containing fibroblast growth factor-1 modified body and application thereof for treating diabetic foot
  • Preparation of gel containing fibroblast growth factor-1 modified body and application thereof for treating diabetic foot
  • Preparation of gel containing fibroblast growth factor-1 modified body and application thereof for treating diabetic foot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 production loading FGF-1 135 gel

[0026] Substrate swelling and sterilization: Add glycerin to Carbomer 940, stir and mix well, fully swell with appropriate amount of water for injection, adjust the pH value to form a transparent gel, and autoclave at 121°C for 30 minutes. Ready to use after cooling;

[0027] Sterilization and filtration of protein: Add recombinant human fibroblast growth factor-l modified protein, human serum albumin and heparin into cooled water for injection, mix well, and filter with a 0.22 μm filter membrane under sterile conditions. Bacteria;

[0028] Gel preparation: under aseptic conditions, fully mix the above two substances, and divide into packages.

[0029] As a matrix material, carbomer has good ductility, is easy to apply and adhere to the skin, is non-irritating to the skin and mucous membranes, and can absorb tissue infiltration fluid, which is conducive to the discharge of secretions; it is non-greasy, releases drugs quick...

Embodiment 2

[0030] Embodiment 2 measures gel activity

[0031]The activity of the gel was determined by tetramethylazolium salt colorimetric method (MTT method). Balb / c3T3 cells were cultured with complete medium at 37°C and 5% CO2 until logarithmic growth phase. Discard the culture medium in the culture flask, digest and collect the cells, add complete medium to adjust the concentration of the cell suspension, so that the density of the cells to be tested is 50,000-100,000 cells / ml, inoculate 100 μl per well in a 96-well cell culture plate , 37 ° C, 5% CO2 culture for 24 h, change the maintenance medium and continue to culture for 24 h. Discard the maintenance solution, add the standard product and the gel sample solution dissolved in the medium and diluted according to a certain concentration, 100 μl per hole, 37. C. Cultivate in a 5% CO2 incubator for 64h to 72h. Add 20 μl of MTT solution to each well and continue to incubate for 5 h. Pour off the liquid, add 100 μl dimethyl sulfox...

Embodiment 3

[0032] Embodiment 3 measures the stability of gel

[0033] By measuring the stability of the gel at different temperatures, the experimental results show that at 4 ° C, 25. C, 37. C stored for 12 months, the gel has no mildew phenomenon, and its appearance, pH value and other physical and chemical indicators have not changed; the biological activity of the drug-loaded gel is poor in stability at room temperature, and the activity after being placed for 3 months There is a more obvious reduction, and the low temperature condition has no obvious influence on the storage of the drug-loaded gel.

[0034] Table 1 Stability test results of FGF-1 gel at 25°C

[0035] time (month) Biological activity (IU / g) Physical and chemical indicators O 2.25XlO 4 --- l 2．12×10 4 no change 2 1．98X10 4 no change 3 1．75×10 4 no change 4 1．45X10 4 no change 5 1．05×lO 4 no change 6 O. 52×10 4 no change

[0036] Table 2 Stab...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of a gel containing a fibroblast growth factor-1 modified body and an application thereof for treating chronic skin ulcer caused by diabetic foot. The gel disclosed by the invention comprises the following components: 20000-80000 IU of recombinant human fibroblast growth factor-1 modified body (FGF-1135) protein, 10 pg to 40 mu g of low molecular weight heparin sodium, 0.2-1.0 g of glycerol, 50-150 mg of a protein protectant, and 0.03-0.05g of a gel substrate, wherein the pH value is adjusted; and water for injection is added to be 10 ml. Compared with the original sequence, the fibroblast growth factor-1 modified body used in the invention is more steady; after being prepared into the gel, the fibroblast growth factor-1 modified body is more convenient for clinical application; the better stability and slow-release effects can be achieved; the bioavailability of protein is increased; the gel is more beneficial to treating diabetic foot; and the gel can be also used for treating burning, scalding and other chronic and refractory ulcers.

Description

technical field [0001] The invention relates to a recombinant human fibroblast growth factor-1 transformant (FGF-1 135 ) The preparation and application of a gel for treating diseases such as diabetic foot and other diseases belong to the field of biomedicine. Background technique [0002] Diabetes mellitus (DM) is a systemic metabolic disease caused by multiple factors. More than 180 million people worldwide suffer from diabetes, which has become a major global public health problem. About 30% to 80% of diabetic patients have skin lesions, which seriously endanger the health of diabetic patients, and the most typical and harmful one is diabetic foot. Diabetic foot (Diabetic Foot, DF) is one of the common chronic complications of DM, which is mainly the result of comprehensive factors based on the disorder of glucose metabolism. Hyperglycemia, hyperlipidemia and their metabolites caused by metabolic abnormalities in diabetes can, on the one hand, cause the degeneration of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/06A61K38/18A61K47/32A61P9/14A61P17/02A61P3/10
Inventor 田海山李校堃王晓杰金利泰姜潮唐禄
Owner 安徽鑫华坤生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com