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Fermentation method of conjugated linoleic acid and strain used in method

A technology of conjugated linoleic acid and linoleic acid isomerase, which is applied in the field of bioengineering, can solve the problems of difficult separation and purification of active isomers, and achieve the goal of eliminating separation and purification steps, reducing production costs and improving production efficiency Effect

Inactive Publication Date: 2013-05-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, the product obtained by this method is a mixture of various isomers, and besides the isomer with physiological activity, it also contains a variety of by-products with unknown safety, and the separation and purification of the active isomer is very difficult

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  • Fermentation method of conjugated linoleic acid and strain used in method
  • Fermentation method of conjugated linoleic acid and strain used in method
  • Fermentation method of conjugated linoleic acid and strain used in method

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Embodiment Construction

[0025] Below in conjunction with the examples, the following examples are illustrative, not restrictive, and the protection scope of the present invention cannot be limited by the following examples.

[0026] The contents of the present invention include: an expression plasmid with a natural linoleic acid isomerase pai gene, a Saccharomyces cerevisiae strain capable of producing t10,c12-CLA containing the PAI expression plasmid, a construction method of the Saccharomyces cerevisiae strain and the Saccharomyces cerevisiae strain Method for the production of t10,c12-CLA by yeast strains.

[0027] The present invention constructs the expression vector pYD1::pai by inserting the pai gene derived from P. acnes into the vector pYD1, and introduces it into Saccharomyces cerevisiae EBY100 to obtain the Saccharomyces cerevisiae strain EBY100-pYD1 containing the expression vector pYD1::pai -pai. After the above strains were induced and cultured, PAI was successfully displayed on the su...

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Abstract

The invention discloses a fermentation method of conjugated linoleic acid and a strain used in method, and relates to a method in which linoleate isomerase is expressed by using a saccharomyces cerevisiae surface display system, and capable of catalytic synthesis of t10, c12-conjugated linoleic acid by using the linoleate isomerase. An expression vector containing a propionibacterium acnes derived linoleate isomerase gene (pai) is constructed and a saccharomyces cerevisiae strain containing the expression vector is obtained. After the induction culture, the linoleate isomerase is displayed and expressed in saccharomyces cerevisiae, and the linoleate isomerase is added into a buffer solution containing substrate linoleic acid for catalytic reaction to generate the t10, c12-conjugated linoleic acid. According to the method provided by the invention, the linoleate isomerase is linked to cytoderm of the saccharomyces cerevisiae by the microorganism surface display system, so that extracellular substrates and the linoleate isomerase can be fully contacted, the catalysis efficiency is improved, a single isomer with physiological activity can be obtained, separation and purification steps are simplified and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to the construction and fermentation of bacterial strains, in particular to a fermentation method of conjugated linoleic acid and the used bacterial strain. Background technique [0002] Conjugated linoleic acid (CLA) is an octadecadienoic acid with a conjugated double bond, which is a series of positions and Generic term for conformational isomers. CLA has various physiological activities such as anti-cancer, anti-atherosclerosis, lowering cholesterol, regulating blood sugar, enhancing immunity, and promoting growth. Among them, t10,c12-CLA is one of the most physiologically active isomers. [0003] In nature, CLA is widely present in ruminant meat and dairy products, but in very low levels. At present, the industrial production of CLA mainly adopts the chemical isomerization method, that is, the base-catalyzed isomerization of linoleic acid. However, the product obtained b...

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Application Information

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IPC IPC(8): C12N1/19C12N15/61C12P7/64C12R1/865
Inventor 刘浩何希宏李凡
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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