Pharmaceutical composition and application thereof
A composition and drug technology, applied in the direction of drug combination, application, active ingredients of hydroxyl compounds, etc., can solve problems such as sugar decomposition and absorption disorders, increased bacterial fermentation gas production, intestinal gas, etc., to achieve good results and less side effects low effect
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Embodiment 1
[0024] Determination of the inhibitory activity of 1-deoxynojirimycin and resveratrol on α-D-glucosidase
[0025] Add 80 μL of sample aqueous solution (sample group) or phosphate buffer solution (control group) into the holes of the 96-well plate according to different groups as shown in Table 1, and add 40 μL of substrate solution (20×10 -3 mol / L p-nitrophenyl-α-D-glucopyranoside (PNPG)), mixed well and placed in a 37°C water bath for pre-incubation for 5 min, then added 40 μL mouse intestinal enzyme initial extract (accurately weighed 100 mg Add rat intestine acetone powder to 1mL 0.9% normal saline, dissolve it by ultrasonic treatment (1 min×3), centrifuge at 3000r / min for 30min, take out the supernatant and dilute it 4 times with distilled water, store it at 4°C for future use), and store it at 37°C After reacting for 15 min, add 0.2 mol / L Na 2 CO 3 160 μL of solution was used to stop the reaction, and the absorbance was measured at 405 nm with a microplate reader. The ...
Embodiment 2
[0034] Effects of Different Dosage Compositions on Blood Glucose in Spontaneously Hyperglycemic Mice
[0035] 1. Drugs: 1-deoxynojirimycin, resveratrol, self-made, prepared to the required concentration with distilled water before the test (the sum of the concentrations of 1-deoxynojirimycin and resveratrol in the composition is the same as the concentration of the prepared white same concentrations of veratrol or 1-deoxynojirimycin). Baitangping, Bayer Pharmaceuticals and Healthcare Co., Ltd.
[0036] 2. Animals: NOD mice, weighing 25-35 g, were purchased from the Experimental Animal Center of Peking University Health Science Center. Blood glucose was measured before the experiment, and animals whose blood glucose value was higher than 11.1mmol / L were selected for the experiment.
[0037] 3. Test methods and results
[0038] Effects on blood glucose in mice: NOD mice were fasted for 6 hours, and blood glucose was measured, and 80 animals with blood glucose values hig...
Embodiment 3
[0045] Effects of Different Dosage Composition on Gastrointestinal Propulsion in Mice
[0046] 1. Experimental animals
[0047] Kunming mice, clean grade, weighing 20g-22g
[0048] 2. experimental method
[0049] 60 Kunming mice fasted for 12 hours were randomly divided into 6 groups according to body weight. After fasting for 18 hours, the following drugs were given by intragastric administration: ① control group (distilled water); ② acarbose group (10mg / kg) 3.-6. Compositions of different proportions (10mg / kg, different drug ratios, the same total amount), after 15min, each mouse was given 0.2ml of 5% charcoal gum arabic suspension by intragastric administration. Shake the 5% charcoal gum arabic suspension vigorously. 20 minutes after gavage with powdered charcoal, the mice were sacrificed by dislocation, the abdominal cavity was opened, and the gastrointestinal tract was taken out. Cut off the mesentery attached to the intestinal tube, and gently spread the intestinal ...
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